GapMind for catabolism of small carbon sources

 

Alignments for a candidate for glcF in Pseudomonas fluorescens FW300-N2E3

Align D-lactate oxidase and glycolate oxidase, iron-sulfur subunit (EC 1.1.3.15) (characterized)
to candidate AO353_21300 AO353_21300 glycolate oxidase iron-sulfur subunit

Query= reanno::psRCH2:GFF3770
         (405 letters)



>FitnessBrowser__pseudo3_N2E3:AO353_21300
          Length = 405

 Score =  617 bits (1591), Expect = 0.0
 Identities = 289/405 (71%), Positives = 345/405 (85%)

Query: 1   MQTNLSEAAKKLPRAEEAESILRSCVHCGFCNATCPTYQLLGDELDGPRGRIYLMKQMFE 60
           MQT LSE AK+LPRA EAESILR+CVHCGFCNATCPTYQLLGDELDGPRGRIYL+KQ+ E
Sbjct: 1   MQTTLSEQAKRLPRAAEAESILRTCVHCGFCNATCPTYQLLGDELDGPRGRIYLIKQVLE 60

Query: 61  GGEVTESTQLHLDRCLTCRNCETTCPSGVKYHNLLDIGRDFIEQQVQRPLGERVVRGGLR 120
           G   T  TQLHLDRCL+CR CETTCPSGV YHNLLDIGR  ++Q V R   +RV+R GLR
Sbjct: 61  GHPATAQTQLHLDRCLSCRACETTCPSGVDYHNLLDIGRAVVDQAVPRSAAQRVLRQGLR 120

Query: 121 TVIPRPGLFKALLGAGNALKPLMPASLKDHLPREIRPAKPRPQVMHSRRVLILEGCVQPS 180
            + P PGLFKALL  G   +PLMP  L+  LPR++ PA   P  +H+R+VL+LEGCVQP 
Sbjct: 121 AMAPNPGLFKALLQIGATFRPLMPRDLEAKLPRDVPPAGNWPTTVHARKVLMLEGCVQPG 180

Query: 181 LSPSTNAAAARVLDRLGISVSPAREAGCCGAVDYHLNAQDAGLDRARRNIDAWWPAIEAG 240
           LSP+TNAAAARVLDRLGISVS   + GCCGA+DYHL+AQ+ GL+RAR+NIDAWWPA+E G
Sbjct: 181 LSPNTNAAAARVLDRLGISVSTVSQVGCCGALDYHLDAQEQGLNRARQNIDAWWPALENG 240

Query: 241 AEAIVQTASGCGAFVKEYGHLLKDDPAYAAKAARVSELAKDLVEVLRSAELEKLNVRADK 300
           AEAIVQTASGCGAF+K+YGHLLK DPAYAAKA RVSE++KDLVE+LR+  LE++    ++
Sbjct: 241 AEAIVQTASGCGAFIKDYGHLLKTDPAYAAKAKRVSEMSKDLVEILRNEPLERVCAATER 300

Query: 301 RMAFHCPCTLQHAQKLGGAVEDVLTRLGYQLTAVPDAHLCCGSAGSYSITQPEISHQLRD 360
           R+AFHCPCTLQHA KLGGAVE++LTRLG+ LT+VPD+HLCCGSAG+YS+TQP ++ QLRD
Sbjct: 301 RIAFHCPCTLQHALKLGGAVEELLTRLGFNLTSVPDSHLCCGSAGTYSLTQPVLARQLRD 360

Query: 361 NKLNALESGKPEVIVTANIGCQTHLDGAGRTPVKHWIEVVEESMQ 405
           N+LNALESG+PE+IVTANIGCQ+HL   GRTPV+HWIE+V++ ++
Sbjct: 361 NRLNALESGRPELIVTANIGCQSHLASVGRTPVRHWIELVDQILR 405


Lambda     K      H
   0.319    0.135    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 631
Number of extensions: 17
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 405
Length of database: 405
Length adjustment: 31
Effective length of query: 374
Effective length of database: 374
Effective search space:   139876
Effective search space used:   139876
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory