Alignments for a candidate for nagPcb in Pseudomonas fluorescens FW300-N2E3

GapMind for catabolism of small carbon sources

Alignments for a candidate for nagPcb in Pseudomonas fluorescens FW300-N2E3

Align PTS system N-acetylglucosamine-specific EIICB component; EIICB-Nag; EC 2.7.1.- (characterized)
to candidate AO353_04465 AO353_04465 PTS N-acetyl-D-glucosamine transporter

Query= SwissProt::O34521
         (452 letters)



>FitnessBrowser__pseudo3_N2E3:AO353_04465
          Length = 571

 Score =  463 bits (1191), Expect = e-135
 Identities = 238/456 (52%), Positives = 311/456 (68%), Gaps = 13/456 (2%)

Query: 5   LQKLGKSFMLPIAVLPAVGIILALGREDVFNIPFVYQAGTAVFDHLPLIFAIGIAIGISK 64
           LQ+LG++ MLPIA+LP  G++L LG  D+ NI  ++ AG  +F +L LIFAIGIA+G ++
Sbjct: 9   LQRLGRALMLPIAILPIAGLLLRLGDTDLLNIAIIHDAGQVIFANLALIFAIGIAVGFAR 68

Query: 65  DSNGAAGLSGAISYLMLDAATKTIDKTNNMAVFGGIIAGLIAGYTYNRFKDTKLPEYLGF 124
           D+NG AGL+GAI YL++ +  K +D + NM +  GII+GL+AG  YNRFKD KLPEYL F
Sbjct: 69  DNNGTAGLAGAIGYLVMVSTLKVLDASINMGMLAGIISGLMAGALYNRFKDIKLPEYLAF 128

Query: 125 FSGRRLVPILTAIITIILAGIFGVVWPPIQSCINSFGEWMLGLGGIGAGIFGLFNRLLIP 184
           F GRR VPI T    + L  IFG++WPPIQ  INSFG+ +L  G IGA +FG+FNRLLI 
Sbjct: 129 FGGRRFVPIATGFSAVGLGVIFGLIWPPIQHGINSFGQLLLESGSIGAFVFGVFNRLLIV 188

Query: 185 LGLHHVLNNIFWFQFGEYNG------VTGDLARFFAKDPTAGTYMTGFFPIMMFGLPAAC 238
            GLHH+LNN+ WF FG +        VTGDLAR+FA DP  G +MTG FP+M+FGLPAAC
Sbjct: 189 TGLHHILNNMAWFIFGSFTDPTTGAIVTGDLARYFAGDPKGGQFMTGMFPMMIFGLPAAC 248

Query: 239 LAMVVTAKPSKRKATAGMMIGFALTAFITGITEPIEFAFMFLSPLLYAVHAVLTGLSLFI 298
           LAM   A P +RK   G+ +  ALT+F+TG+TEPIEFAFMFL+PLLY +H +LTG+++ I
Sbjct: 249 LAMYRNALPERRKVMGGIFLSMALTSFLTGVTEPIEFAFMFLAPLLYLLHVLLTGMAMAI 308

Query: 299 VNWLGIRSGFSFSAGAIDYVLSYGIAEKPLLLLLVGICYAAVYFIVFYVLIKALNLKTPG 358
            N L I  GF+FS GAID  L +G +    L+  VG+ YA +Y++VF   I+  NLKTPG
Sbjct: 309 TNALNIHLGFTFSGGAIDMALGWGKSTNGWLVFPVGLAYAVIYYVVFDFCIRRFNLKTPG 368

Query: 359 REDDDVDE--VLDENTVQDVNENIMLKGLGGKENLQTIDHCATRLRLTVKDTALVDEALL 416
           RE   V E  VL EN          ++ LGG ENL T+  C TRLRL + D     ++ L
Sbjct: 369 REGVVVGEKVVLSENQ----RAGAYIQALGGAENLITVGACTTRLRLEMVDRNKASDSEL 424

Query: 417 KKAGAKGVVKSG-GQSVQVIIGPNVEFAAEELRAAV 451
           K  GA  VV+ G G S+QV++GP  +  A+E+R A+
Sbjct: 425 KALGAMAVVRPGKGGSLQVVVGPLADSIADEIRQAM 460



 Score = 35.4 bits (80), Expect = 5e-06
 Identities = 23/71 (32%), Positives = 34/71 (47%), Gaps = 1/71 (1%)

Query: 382 LKGLGGKENLQTIDHCA-TRLRLTVKDTALVDEALLKKAGAKGVVKSGGQSVQVIIGPNV 440
           L  +GG +N+  +D  A TR+RL + D   + E  LK  G +GV    G    ++IG   
Sbjct: 492 LNAVGGSDNVLQLDCVAMTRIRLQLADGKALSECQLKDLGCQGVSALDGGVWHLLIGDKA 551

Query: 441 EFAAEELRAAV 451
              +E L   V
Sbjct: 552 LSLSEALEGLV 562


Lambda     K      H
   0.326    0.144    0.429 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 701
Number of extensions: 36
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 452
Length of database: 571
Length adjustment: 34
Effective length of query: 418
Effective length of database: 537
Effective search space:   224466
Effective search space used:   224466
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources