Alignments for a candidate for araG in Pseudomonas fluorescens FW300-N2E3

GapMind for catabolism of small carbon sources

Alignments for a candidate for araG in Pseudomonas fluorescens FW300-N2E3

Align L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 (characterized)
to candidate AO353_20820 AO353_20820 sugar ABC transporter ATP-binding protein

Query= CharProtDB::CH_014279
         (504 letters)



>FitnessBrowser__pseudo3_N2E3:AO353_20820
          Length = 517

 Score =  339 bits (870), Expect = 1e-97
 Identities = 194/473 (41%), Positives = 277/473 (58%), Gaps = 6/473 (1%)

Query: 8   LSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGAGKSTLLKILSGNYAPTTGSVVIN 67
           LS  GIGKT+     LT I      G+V AL GENGAGKSTL KI+ G   PTTG +   
Sbjct: 10  LSVSGIGKTY-AQPVLTGIDLTLMRGEVLALTGENGAGKSTLSKIIGGLVTPTTGQMQFQ 68

Query: 68  GQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAENIYLGQLPHKGGIVNRSLLNYEAGLQ 127
           G++      T A   G+ ++ QEL+L+P ++VAEN++L  LP  GG ++R  L   A   
Sbjct: 69  GRDYRPGSRTQAEELGIRMVMQELNLLPTLSVAENLFLDNLPSNGGWISRKQLRKAAIAA 128

Query: 128 LKHLGMD-IDPDTPLKYLSIGQWQMVEIAKALARNAKIIAFDEPTSSLSAREIDNLFRVI 186
           +  +G+D IDPDT +  L IG  QMVEIA+ L  +  ++  DEPT+ L+ARE++ LF  I
Sbjct: 129 MAQVGLDAIDPDTLVGELGIGHQQMVEIARNLIGDCHVLILDEPTAMLTAREVEMLFEQI 188

Query: 187 RELRKEGRVILYVSHRMEEIFALSDAITVFKDGRYVKTFTDMQQVDHDALVQAMVGRDIG 246
             L+  G  I+Y+SHR+EE+  ++  I V +DG  V     M   + + LV  MVGR++G
Sbjct: 189 TRLQARGVAIIYISHRLEELARVAQRIAVLRDGNLV-CVEPMANYNSEQLVNLMVGRELG 247

Query: 247 DIYGWQPRSYGEERLRLDAVKAPGVRTPISLAVRSGEIVGLFGLVGAGRSELMKGMFGGT 306
           +     PR  G   L +  +        +S  VRSGEI G+ GL+GAGR+EL++ +FG  
Sbjct: 248 EHIDLGPRHIGAPALTVKGLSRSDKVRDVSFEVRSGEIFGISGLIGAGRTELLRLIFGAD 307

Query: 307 QITAGQVYIDQ--QPIDIRKPSHAIAAGMMLCPEDRKAEGIIPVHSVRDNINISARRKHV 364
              +G V +    Q + IR P+ A+  G+ L  EDRK EG++   S+  NI +      +
Sbjct: 308 AADSGTVALGSPAQVVSIRSPADAVGHGIALITEDRKGEGLLLTQSISANIALG-NMPVI 366

Query: 365 LGGCVINNGWEENNADHHIRSLNIKTPGAEQLIMNLSGGNQQKAILGRWLSEEMKVILLD 424
             G ++N+G E   A   I ++ I++    QL+  LSGGNQQK ++GRWL  E  V+L D
Sbjct: 367 SSGGIVNSGDEMALAQRQIDAMRIRSSSPAQLVSELSGGNQQKVVIGRWLERECSVLLFD 426

Query: 425 EPTRGIDVGAKHEIYNVIYALAAQGVAVLFASSDLPEVLGVADRIVVMREGEI 477
           EPTRGIDVGAK +IY ++  L  QG A++  SSDL E++ + DRI V+  G +
Sbjct: 427 EPTRGIDVGAKFDIYALLGELTRQGKALVVVSSDLRELMLICDRIGVLSAGRL 479



 Score = 94.4 bits (233), Expect = 9e-24
 Identities = 67/245 (27%), Positives = 120/245 (48%), Gaps = 12/245 (4%)

Query: 6   PYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGAGKSTLLKILSGNYAPTTGSVV 65
           P L+ +G+ ++      + D+SF+  +G++  + G  GAG++ LL+++ G  A  +G+V 
Sbjct: 260 PALTVKGLSRS----DKVRDVSFEVRSGEIFGISGLIGAGRTELLRLIFGADAADSGTVA 315

Query: 66  ING--QEMSFSDTTAALNAGVAIIYQELH---LVPEMTVAENIYLGQLP--HKGGIVNRS 118
           +    Q +S      A+  G+A+I ++     L+   +++ NI LG +P    GGIVN  
Sbjct: 316 LGSPAQVVSIRSPADAVGHGIALITEDRKGEGLLLTQSISANIALGNMPVISSGGIVNSG 375

Query: 119 LLNYEAGLQLKHLGM-DIDPDTPLKYLSIGQWQMVEIAKALARNAKIIAFDEPTSSLSAR 177
                A  Q+  + +    P   +  LS G  Q V I + L R   ++ FDEPT  +   
Sbjct: 376 DEMALAQRQIDAMRIRSSSPAQLVSELSGGNQQKVVIGRWLERECSVLLFDEPTRGIDVG 435

Query: 178 EIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKDGRYVKTFTDMQQVDHDALV 237
              +++ ++ EL ++G+ ++ VS  + E+  + D I V   GR + TF        D L 
Sbjct: 436 AKFDIYALLGELTRQGKALVVVSSDLRELMLICDRIGVLSAGRLIDTFERDSWTQDDLLA 495

Query: 238 QAMVG 242
            A  G
Sbjct: 496 AAFAG 500


Lambda     K      H
   0.319    0.136    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 676
Number of extensions: 28
Number of successful extensions: 10
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 504
Length of database: 517
Length adjustment: 35
Effective length of query: 469
Effective length of database: 482
Effective search space:   226058
Effective search space used:   226058
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources