Align 2-ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26) (characterized)
to candidate AO353_14870 AO353_14870 ketoglutarate semialdehyde dehydrogenase
Query= metacyc::MONOMER-18709 (527 letters) >FitnessBrowser__pseudo3_N2E3:AO353_14870 Length = 529 Score = 788 bits (2036), Expect = 0.0 Identities = 401/523 (76%), Positives = 450/523 (86%) Query: 1 MTAILGHNFIGGARSAAGTLFLQSLDAASGEALPYRFVQATPEEVDAAAEAAASAYPHYR 60 M+AI+GHNFIG RSAAG L S+DA SGE LPY F QA+ EE+D AA AA+AYP YR Sbjct: 1 MSAIIGHNFIGFGRSAAGDHVLHSVDAHSGEPLPYDFSQASAEEIDNAAHFAATAYPIYR 60 Query: 61 QLPASRRAEFLDTIAAELDALDDDFVAIVCRETALPATRIQGERSRTSGQLRLFAEVLRR 120 LPA+ RA FL+TIA E+DAL DDF+AIVCRETALPA RIQGER+RTSGQLRLFA VLR+ Sbjct: 61 NLPATVRAGFLETIAEEIDALGDDFIAIVCRETALPAARIQGERARTSGQLRLFARVLRQ 120 Query: 121 GDFHGARIDRARPERKPLPRVDLRQCRIGLGPVAVFGASNFPLAFSTAGGDTAAALAAGC 180 GDF GARIDRA PER+P PR D+RQ RIGLGPVAVFGASNFPLAFSTAGGDTAAALAAGC Sbjct: 121 GDFLGARIDRALPERQPFPRPDVRQWRIGLGPVAVFGASNFPLAFSTAGGDTAAALAAGC 180 Query: 181 PVVFKAHSGHMATAERVAAAILRAAERTGMPAGVFNMIYGGGVGERLVRHPAIQAVGFTG 240 PVVFKAHSGHMATAE VAAAI+RAA+R+GMP GVFNMIYG GVGE LV+HPAIQAVGFTG Sbjct: 181 PVVFKAHSGHMATAELVAAAIIRAAQRSGMPEGVFNMIYGAGVGEALVKHPAIQAVGFTG 240 Query: 241 SLKGGRALCDMAAARAQPIPVFAEMSSINPVVLLPAALKKRGEAVADELSASVVLGCGQF 300 SLKGGRALCDMAAAR QPIPVFAEMSSINPV+LLPAAL R ++A ELSAS+VLG GQF Sbjct: 241 SLKGGRALCDMAAARPQPIPVFAEMSSINPVLLLPAALAARSASIASELSASIVLGGGQF 300 Query: 301 CTNPGLVIGIRSAQFSAFLERFAARMDDQPAQTMLNTGTLASYEKGLAALHAHPRVRHLA 360 CTNPGL+IG+RSA FSAF+E+ A+++++P QT+LN+GT SYE G+ L HPRV L+ Sbjct: 301 CTNPGLIIGLRSAAFSAFIEQLTAQINEKPPQTLLNSGTRRSYESGVQRLLEHPRVTRLS 360 Query: 361 GQPQEGRQARPQLFQADVSLLLEGDELLQEEVFGPASVVVEVADHAELKRALHGLHGQLT 420 G G Q +PQLF+ADVSLLLEGDE+LQEEVFGPAS+V+EVAD EL+ AL LHGQLT Sbjct: 361 GNLVPGNQVQPQLFKADVSLLLEGDEVLQEEVFGPASIVLEVADERELRLALCALHGQLT 420 Query: 421 ATLIAEAEDLASFADLVPLLEQKAGRLLLNGYPTGVEVCDAMVHGGPYPATSDARGTSVG 480 ATLIAE +DL A L+P+LEQKAGRLLLNGYPTGVEVCDAMVHGGPYPATSDARGTSVG Sbjct: 421 ATLIAEPQDLVLCAQLIPILEQKAGRLLLNGYPTGVEVCDAMVHGGPYPATSDARGTSVG 480 Query: 481 TLAIDRFLRPVCYQNYPDAWLPEALKDGNPLGIARLVDGIVTR 523 +LAI+RFLRPVCYQN PD LP+ALK+ NPLGI RLVDG+ TR Sbjct: 481 SLAIERFLRPVCYQNCPDRLLPDALKNANPLGILRLVDGVNTR 523 Lambda K H 0.321 0.136 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 864 Number of extensions: 25 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 527 Length of database: 529 Length adjustment: 35 Effective length of query: 492 Effective length of database: 494 Effective search space: 243048 Effective search space used: 243048 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory