Align arginine N-succinyltransferase; EC 2.3.1.109 (characterized)
to candidate AO353_25630 AO353_25630 arginine N-succinyltransferase
Query= CharProtDB::CH_107315 (338 letters) >FitnessBrowser__pseudo3_N2E3:AO353_25630 Length = 344 Score = 246 bits (629), Expect = 5e-70 Identities = 134/337 (39%), Positives = 203/337 (60%), Gaps = 3/337 (0%) Query: 2 LVMRPAQAADLPQVQRLAADSPVGVTSLPDDAERLRDKILASEASFAAEVSYNGEESYFF 61 +++RPA DLP + LA + G+T+LP DA RLR ++ +E +FA E + Y F Sbjct: 1 MIVRPATMNDLPALLALAHSAGAGLTTLPADAGRLRQRLEWAEKTFAGEAE-RADADYLF 59 Query: 62 VLEDSASGELVGCSAIVASAGFSEPFYSFRNETFVHASRSLSIHNKIHVLSLCHDLTGNS 121 VLE + +GE VG A+ + G EP+Y++R FV AS++L I+ ++ L L +D+TG S Sbjct: 60 VLE-ADNGEAVGICALAGAVGLREPWYNYRMGLFVAASKNLGINQQLLTLFLGNDMTGQS 118 Query: 122 LLTSFYVQRDLVQSVYAELNSRGRLLFMASHPERFADAVVVEIVGYSDEQGESPFWNAVG 181 L S ++ + L S+ R LF+A + F D V+ E+ GYSDE G SPFW +G Sbjct: 119 ELCSLFLHAGHRSGLNGRLLSKARFLFLAEFRQYFGDKVIAEMRGYSDEDGVSPFWEGLG 178 Query: 182 RNFFDLNYIEAEKLSGLKSRTFLAELMPHYPIYVPLLPDAAQESMGQVHPRAQITFDILM 241 R+FF +++ +A+ L+GL ++TF+AELMP +P+ LLP+AA+ ++G+VHP + +L Sbjct: 179 RHFFKMDFADADYLTGLGNKTFIAELMPKFPLPTCLLPEAARATIGRVHPNTEPALGMLK 238 Query: 242 REGFETDNYIDIFDGGPTLHARTSGIRSIAQSRVVPVKIGEAPKSGRPYLVTNGQLQDFR 301 EGFE +YIDIFDGGP + T IR++ S+V+ + IG + YL+ N Q D R Sbjct: 239 AEGFEHKDYIDIFDGGPLIECATGDIRAVRDSQVLQLSIGTPGEQAAVYLIHNRQFADCR 298 Query: 302 AVVLDLDWAPGKPVALSVEAAEALGVGEGASVRLVAV 338 A G + + A+ALG+ GASVR V++ Sbjct: 299 ITAAPARVAAG-TLIVDAHTAKALGLTAGASVRAVSL 334 Lambda K H 0.319 0.135 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 275 Number of extensions: 17 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 338 Length of database: 344 Length adjustment: 29 Effective length of query: 309 Effective length of database: 315 Effective search space: 97335 Effective search space used: 97335 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 49 (23.5 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory