GapMind for catabolism of small carbon sources

 

Aligments for a candidate for puuB in Pseudomonas fluorescens FW300-N2E3

Align gamma-glutamylputrescine oxidase (EC 1.4.3.-) (characterized)
to candidate AO353_20720 AO353_20720 gamma-glutamylputrescine oxidoreductase

Query= reanno::pseudo5_N2C3_1:AO356_21495
         (427 letters)



>lcl|FitnessBrowser__pseudo3_N2E3:AO353_20720 AO353_20720
           gamma-glutamylputrescine oxidoreductase
          Length = 427

 Score =  819 bits (2116), Expect = 0.0
 Identities = 398/427 (93%), Positives = 417/427 (97%)

Query: 1   MANTPYPESYYAASANPVPPRPALQDDVETDVCVIGAGYTGLSSALFLLENGFKVTVLEA 60
           MANTPYPESYYAASAN  P RP LQDDVETD+CVIGAGYTGLSSALFLLE+GF+VTVLEA
Sbjct: 1   MANTPYPESYYAASANAAPLRPMLQDDVETDICVIGAGYTGLSSALFLLESGFRVTVLEA 60

Query: 61  AKVGFGASGRNGGQIVNSYSRDIDVIERSVGPQQAQLLGNMAFEGGRIIRERVAKYQIQC 120
           AKVGFGASGRNGGQIVNSYSRDIDVIERSVGP+QAQ+LG MAFEGG+IIR+RVA+YQIQC
Sbjct: 61  AKVGFGASGRNGGQIVNSYSRDIDVIERSVGPKQAQMLGQMAFEGGKIIRDRVARYQIQC 120

Query: 121 DLKDGGVFAALTAKQMGHLESQKRLWERFGHTQLELLDQRRIREVVACEEYVGGMLDMSG 180
           DLKDGGVFAA+T+KQMGHLESQKRLWER+GHTQLELLD+RRIREVVAC++YVGGMLDMSG
Sbjct: 121 DLKDGGVFAAITSKQMGHLESQKRLWERYGHTQLELLDKRRIREVVACDQYVGGMLDMSG 180

Query: 181 GHIHPLNLALGEAAAVESLGGVIYEQSPAVRIERGASPVVHTPQGKVRAKFIIVAGNAYL 240
           GHIHPLNLALGEAAAVESLGG IYEQSPA+RIERGA+PVVHTPQGKVRAKFIIVAGNAYL
Sbjct: 181 GHIHPLNLALGEAAAVESLGGTIYEQSPAIRIERGANPVVHTPQGKVRAKFIIVAGNAYL 240

Query: 241 GNLVPELAAKSMPCGTQVIATEPLGDELAHSLLPQDYCVEDCNYLLDYYRLTGDKRLIFG 300
           GNLVPELAAKSMPCGTQVI TEPLGDELA SLLPQDYCVEDCNYLLDYYRL+GDKRLIFG
Sbjct: 241 GNLVPELAAKSMPCGTQVITTEPLGDELAKSLLPQDYCVEDCNYLLDYYRLSGDKRLIFG 300

Query: 301 GGVVYGARDPANIEAIIRPKMLKAFPQLKDVKIDYAWTGNFLLTLSRLPQVGRLGDNIYY 360
           GGVVYGARDPANIEAIIRPKMLKAFPQL+DVKIDYAWTGNFLLTLSRLPQVGRLGDNIYY
Sbjct: 301 GGVVYGARDPANIEAIIRPKMLKAFPQLEDVKIDYAWTGNFLLTLSRLPQVGRLGDNIYY 360

Query: 361 SQGCSGHGVTYTHLAGKVLAEALRGQAERFDAFADLPHYPFPGGQLLRTPFAAMGAWYYG 420
           SQGCSGHGVTYTHLAGKVLAEALRGQAERFDAFADLPHYPFPGGQLLRTPFAA+GAWYYG
Sbjct: 361 SQGCSGHGVTYTHLAGKVLAEALRGQAERFDAFADLPHYPFPGGQLLRTPFAALGAWYYG 420

Query: 421 LRDKLGF 427
           LRDK  F
Sbjct: 421 LRDKFDF 427


Lambda     K      H
   0.320    0.139    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 864
Number of extensions: 20
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 427
Length of database: 427
Length adjustment: 32
Effective length of query: 395
Effective length of database: 395
Effective search space:   156025
Effective search space used:   156025
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory