GapMind for catabolism of small carbon sources

 

Alignments for a candidate for aatP in Pseudomonas fluorescens FW300-N2E3

Align Glutamate/aspartate transport ATP-binding protein GltL aka B0652, component of Glutamate/aspartate porter (characterized)
to candidate AO353_11610 AO353_11610 glutamine ABC transporter ATP-binding protein

Query= TCDB::P0AAG3
         (241 letters)



>FitnessBrowser__pseudo3_N2E3:AO353_11610
          Length = 260

 Score =  260 bits (664), Expect = 2e-74
 Identities = 127/238 (53%), Positives = 173/238 (72%)

Query: 1   MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLIKTVNGLEPVQQGEITV 60
           +I  K  +K++G  QVL+     VK GEV+V+ GPSG GKSTL++ +NGLE    GE+  
Sbjct: 4   LIEFKGFNKFFGEHQVLSGIDLSVKTGEVIVILGPSGCGKSTLLRCLNGLEVAHSGELRF 63

Query: 61  DGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQVKVLKRDKAPAREKALKL 120
            G  +  K TD  ++R ++GMVFQ + LFPH+S+++NL L  +KV KR    ARE+AL L
Sbjct: 64  AGRELLSKTTDWREVRQQIGMVFQSYHLFPHMSVLDNLLLGPLKVQKRPAREAREQALAL 123

Query: 121 LERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLFDEPTSALDPEMINEVLDVMV 180
           LERVGL+     FP QLSGGQQQR+AI R+LCM+P  MLFDE T+ALDPEM+ EVL+V+ 
Sbjct: 124 LERVGLADKREAFPRQLSGGQQQRIAIIRSLCMNPQVMLFDEVTAALDPEMVKEVLEVIQ 183

Query: 181 ELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDSPKDAFFDDPKSDRAKDFLAK 238
            LA +GMT+++VTHEM FAR VA+R++FMD G+I+E +P + FF +P++ RA+ FL K
Sbjct: 184 GLARDGMTLLIVTHEMAFARAVADRIVFMDAGRILEQNPPEIFFTNPQTARAQQFLEK 241


Lambda     K      H
   0.320    0.135    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 168
Number of extensions: 7
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 241
Length of database: 260
Length adjustment: 24
Effective length of query: 217
Effective length of database: 236
Effective search space:    51212
Effective search space used:    51212
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory