Alignments for a candidate for cimH in Pseudomonas fluorescens FW300-N2E3

GapMind for catabolism of small carbon sources

Alignments for a candidate for cimH in Pseudomonas fluorescens FW300-N2E3

Align L-malate/citrate:H+ symporter (electroneutral) (characterized)
to candidate AO353_24495 AO353_24495 malate permease

Query= TCDB::P94363
         (450 letters)



>FitnessBrowser__pseudo3_N2E3:AO353_24495
          Length = 447

 Score =  331 bits (848), Expect = 3e-95
 Identities = 161/418 (38%), Positives = 259/418 (61%)

Query: 32  KVGIIPLPVYALLFILITVFVMHHDVKSDILTSIAVMAFFGFTFAQIGKSIPIVRSIGGP 91
           ++G+IPLP++  + +++ +      + ++++  +AV+   G  F Q+G  +PI++ IGG 
Sbjct: 29  EIGVIPLPIFLGIAVIVYLSAHLGFLPNNMIGGLAVIMTMGMFFGQLGSRLPILKEIGGG 88

Query: 92  AILATFIPSAVVYYHLLPNDIVKSTTEFTENSNFLYLFIAGIVVGSILGMKRETLVKAFM 151
           AIL   +PS +V+Y       + +T    + +NFLY  IA +VVGSILGM R  L++  M
Sbjct: 89  AILCLMLPSILVFYGFFGPATIDATKMLMKEANFLYFVIASLVVGSILGMSRFILIQGMM 148

Query: 152 KIFIPLIVGSVTAAIVGLAVGTLLGLGFQHTLLYIVIPIMAGGVGEGAIPLSIGYSDIMP 211
           ++FIPL+VG++ A   GL VG L+G  F HT  +I++PI+ GG+GEG +PLS+ YS I+ 
Sbjct: 149 RMFIPLLVGTLAAVASGLIVGKLVGYSFYHTFFFIIVPIIGGGIGEGILPLSLAYSAILG 208

Query: 212 ISQGEAFALVLPSIMLGSLCAIILAGLLNRIGKKKPEWTGNGKVDRSEEESPALEESQSG 271
            +     A ++P+ ++G++ AII AG L R+  K+P   G G + R+++E+   +  +  
Sbjct: 209 GTPDIYVAKLVPAAVVGNIAAIICAGYLARLALKRPHLNGEGSLIRAKDENDKFQAREDN 268

Query: 272 QQMFNLSLFASGGILAVSLYLVGMLAHDFFGFPAPVAMLLLAVLIKLFRLVPASIENGAF 331
               +  +  +G ++  + +++G L     G P PV M+L AVL K  R++P  +E G  
Sbjct: 269 GSTIDFRVIGAGILVICAFFVLGGLLEKILGVPGPVLMILAAVLFKYIRVLPEKLEKGTN 328

Query: 332 GVSRFFSTAVTYPLLFAIGVSMTPWDKLVAAFNLSNIITILSVVVTMMAVGFFTGKWLNM 391
              +  S+A  +P++  +G+   P D +V  F++S ++  +SVV +M   GFF G  + M
Sbjct: 329 AFYKLISSAFIWPVMIGLGMLYVPLDSVVKVFSISYVLVCVSVVASMAIAGFFIGNLMKM 388

Query: 392 YPIETAIINACHSGQGGTGDVAILSAAERLELMPFAQVSTRIGGAITVSLTLLLLHQF 449
           YPIE+AI+  CHSG GGTGDVAILSA+ R+ LMPFAQ+STR+GGA TV +  +LL  F
Sbjct: 389 YPIESAIVTCCHSGLGGTGDVAILSASNRMSLMPFAQISTRLGGASTVIIASILLRIF 446


Lambda     K      H
   0.326    0.141    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 601
Number of extensions: 26
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 450
Length of database: 447
Length adjustment: 33
Effective length of query: 417
Effective length of database: 414
Effective search space:   172638
Effective search space used:   172638
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources