GapMind for catabolism of small carbon sources

 

Alignments for a candidate for atoB in Pseudomonas fluorescens FW300-N2E3

Align Acetyl-CoA C-acetyltransferase (EC 2.3.1.9) (characterized)
to candidate AO353_27530 AO353_27530 acetyl-CoA acetyltransferase

Query= reanno::pseudo13_GW456_L13:PfGW456L13_2411
         (393 letters)



>FitnessBrowser__pseudo3_N2E3:AO353_27530
          Length = 394

 Score =  564 bits (1454), Expect = e-165
 Identities = 285/394 (72%), Positives = 320/394 (81%), Gaps = 1/394 (0%)

Query: 1   MNTPEIYVVSAARTAIGTFGGSLKDVPLADLATTAVKAALERAAVDPALVGHLVMGNVIP 60
           MN  +I+VVSA R+AIG+FGGSLKDVP   LAT   +AA+ER+ + P  +GH VMG+VIP
Sbjct: 1   MNPSDIFVVSAVRSAIGSFGGSLKDVPPIQLATDVCRAAIERSGLAPEHIGHAVMGHVIP 60

Query: 61  TETRDAYISRVAAMNAGIPKETPAYNVNRLCGSGLQAIINAAQTLMLGDADIVVGAGAES 120
           TE RDAYISR  AMNAG+PKETPA+NVNRLCGSGLQAI++AAQ+LMLGD    +  G ES
Sbjct: 61  TEARDAYISRAVAMNAGLPKETPAFNVNRLCGSGLQAIVSAAQSLMLGDTGAALAGGVES 120

Query: 121 MSRGPYLMPAARWGSRMGNAQVIDYMLGILHDPFHGIHMGITAENVAARNGITREMQDAL 180
           MSRG YL+P ARWG+RMG+ Q IDYMLG+L DPF G HMGITAEN+A   GI+R+ QD L
Sbjct: 121 MSRGAYLLPQARWGARMGDIQGIDYMLGVLQDPFAGFHMGITAENIAEHYGISRQTQDQL 180

Query: 181 AFEDQQRAAHAIANGYFSEQIATVEIQDRKGVKLFSVDEHPRA-TSLEQLAAMKPAFKKD 239
           A   QQRAA AIA G F+ QI  +E+  RKG   F+ DEH RA  S EQL+ MKPAFKKD
Sbjct: 181 ALLSQQRAARAIAEGRFTGQIVPIEVASRKGTVSFATDEHVRAEVSFEQLSGMKPAFKKD 240

Query: 240 GSVTAGNASGLNDGAAALVMASGNAVQANNLKPLARLVSYAHAGVEPEFMGLGPIPATRL 299
           GSVTAGNASGLNDGA AL+MA+G  VQ   LKP+ARLV YAHAGVEP  MGLGPIPATRL
Sbjct: 241 GSVTAGNASGLNDGAGALIMATGQVVQEQGLKPMARLVGYAHAGVEPSMMGLGPIPATRL 300

Query: 300 ALKRAGLTVADLDVIEANIAFAAQACAVSQELDLDPAKVNPNGSGIALGHPVGATGAIIA 359
            LKRAGLTVADLDVIE+N AFAAQACAV+QEL  DP KVNPNGSGI+LGHPVGATGAIIA
Sbjct: 301 VLKRAGLTVADLDVIESNEAFAAQACAVAQELGFDPEKVNPNGSGISLGHPVGATGAIIA 360

Query: 360 TKAIHELHRTGGRYALVTMCIGGGQGIAAIFERV 393
           TKAIHELHR  GRYAL TMCIGGGQGIA +FERV
Sbjct: 361 TKAIHELHRIQGRYALATMCIGGGQGIAVLFERV 394


Lambda     K      H
   0.318    0.133    0.378 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 537
Number of extensions: 14
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 393
Length of database: 394
Length adjustment: 31
Effective length of query: 362
Effective length of database: 363
Effective search space:   131406
Effective search space used:   131406
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory