Alignments for a candidate for fruK in Pseudomonas fluorescens FW300-N2E3

GapMind for catabolism of small carbon sources

Alignments for a candidate for fruK in Pseudomonas fluorescens FW300-N2E3

Align Fructose import ATP-binding protein FruK; EC 7.5.2.- (characterized)
to candidate AO353_20820 AO353_20820 sugar ABC transporter ATP-binding protein

Query= SwissProt::Q8G847
         (513 letters)



>FitnessBrowser__pseudo3_N2E3:AO353_20820
          Length = 517

 Score =  300 bits (769), Expect = 6e-86
 Identities = 173/470 (36%), Positives = 266/470 (56%), Gaps = 9/470 (1%)

Query: 23  LDGVDLTLYPGEVHALMGENGAGKSTMIKALTGVYKINAGSIMVDGKPQQFNGTLDAQNA 82
           L G+DLTL  GEV AL GENGAGKST+ K + G+     G +   G+  +      A+  
Sbjct: 24  LTGIDLTLMRGEVLALTGENGAGKSTLSKIIGGLVTPTTGQMQFQGRDYRPGSRTQAEEL 83

Query: 83  GIATVYQEVNLCTNLSVGENVMLGHEKRGPFGIDWKKTHEAAKKYLAQMGLESIDPHTPL 142
           GI  V QE+NL   LSV EN+ L +       I  K+  +AA   +AQ+GL++IDP T +
Sbjct: 84  GIRMVMQELNLLPTLSVAENLFLDNLPSNGGWISRKQLRKAAIAAMAQVGLDAIDPDTLV 143

Query: 143 SSISIAMQQLVAIARAMVINAKVLILDEPTSSLDANEVRDLFAIMRKVRDSGVAILFVSH 202
             + I  QQ+V IAR ++ +  VLILDEPT+ L A EV  LF  + +++  GVAI+++SH
Sbjct: 144 GELGIGHQQMVEIARNLIGDCHVLILDEPTAMLTAREVEMLFEQITRLQARGVAIIYISH 203

Query: 203 FLDQIYEITDRLTILRNGQFIKEVMTKDTPRDELIGMMIGKSAAELSQIGAKKARREITP 262
            L+++  +  R+ +LR+G  +      +   ++L+ +M+G+   E   +G +        
Sbjct: 204 RLEELARVAQRIAVLRDGNLVCVEPMANYNSEQLVNLMVGRELGEHIDLGPRHI------ 257

Query: 263 GEKPIVDVKGLGKKGTINPVDVDIYKGEVVGFAGLLGSGRTELGRLLYGADKPDSGTYTL 322
              P + VKGL +   +  V  ++  GE+ G +GL+G+GRTEL RL++GAD  DSGT  L
Sbjct: 258 -GAPALTVKGLSRSDKVRDVSFEVRSGEIFGISGLIGAGRTELLRLIFGADAADSGTVAL 316

Query: 323 N--GKKVNISDPYTALKNKIAYSTENRRDEGIIGDLTVRQNILIALQATRGMFKPIPKKE 380
               + V+I  P  A+ + IA  TE+R+ EG++   ++  NI +           +   +
Sbjct: 317 GSPAQVVSIRSPADAVGHGIALITEDRKGEGLLLTQSISANIALGNMPVISSGGIVNSGD 376

Query: 381 ADAIVDKYMKELNVRPADPDRPVKNLSGGNQQKVLIGRWLATHPELLILDEPTRGIDIGA 440
             A+  + +  + +R + P + V  LSGGNQQKV+IGRWL     +L+ DEPTRGID+GA
Sbjct: 377 EMALAQRQIDAMRIRSSSPAQLVSELSGGNQQKVVIGRWLERECSVLLFDEPTRGIDVGA 436

Query: 441 KAEIQQVVLDLASQGMGVVFISSELEEVVRLSDDIEVLKDRHKIAEIEND 490
           K +I  ++ +L  QG  +V +SS+L E++ + D I VL     I   E D
Sbjct: 437 KFDIYALLGELTRQGKALVVVSSDLRELMLICDRIGVLSAGRLIDTFERD 486



 Score = 73.6 bits (179), Expect = 2e-17
 Identities = 56/220 (25%), Positives = 103/220 (46%), Gaps = 9/220 (4%)

Query: 26  VDLTLYPGEVHALMGENGAGKSTMIKALTGVYKINAGSIMVDGKPQQ---FNGTLDAQNA 82
           V   +  GE+  + G  GAG++ +++ + G    ++G++ + G P Q        DA   
Sbjct: 276 VSFEVRSGEIFGISGLIGAGRTELLRLIFGADAADSGTVAL-GSPAQVVSIRSPADAVGH 334

Query: 83  GIATVYQE---VNLCTNLSVGENVMLGHEKRGPFG--IDWKKTHEAAKKYLAQMGLESID 137
           GIA + ++     L    S+  N+ LG+      G  ++       A++ +  M + S  
Sbjct: 335 GIALITEDRKGEGLLLTQSISANIALGNMPVISSGGIVNSGDEMALAQRQIDAMRIRSSS 394

Query: 138 PHTPLSSISIAMQQLVAIARAMVINAKVLILDEPTSSLDANEVRDLFAIMRKVRDSGVAI 197
           P   +S +S   QQ V I R +     VL+ DEPT  +D     D++A++ ++   G A+
Sbjct: 395 PAQLVSELSGGNQQKVVIGRWLERECSVLLFDEPTRGIDVGAKFDIYALLGELTRQGKAL 454

Query: 198 LFVSHFLDQIYEITDRLTILRNGQFIKEVMTKDTPRDELI 237
           + VS  L ++  I DR+ +L  G+ I         +D+L+
Sbjct: 455 VVVSSDLRELMLICDRIGVLSAGRLIDTFERDSWTQDDLL 494


Lambda     K      H
   0.316    0.135    0.376 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 604
Number of extensions: 32
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 513
Length of database: 517
Length adjustment: 35
Effective length of query: 478
Effective length of database: 482
Effective search space:   230396
Effective search space used:   230396
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources