GapMind for catabolism of small carbon sources

 

L-fucose catabolism in Pseudomonas fluorescens FW300-N2E3

Best path

HSERO_RS05250, HSERO_RS05255, HSERO_RS05260, fucU, fucI, fucK, fucA, tpi, aldA

Also see fitness data for the top candidates

Rules

Overview: Fucose degradation in GapMind is based on the MetaCyc pathway via L-fuculose (link) or the oxidative pathway via 2,4-diketo-3-deoxy-L-fuconate (KDF) hydrolase (PMC6336799).

23 steps (13 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
HSERO_RS05250 ABC transporter for L-fucose, ATPase component AO353_20820 AO353_21385
HSERO_RS05255 ABC transporter for L-fucose, permease component AO353_20825 AO353_21390
HSERO_RS05260 ABC transporter for L-fucose, substrate-binding component
fucU L-fucose mutarotase FucU
fucI L-fucose isomerase FucI
fucK L-fuculose kinase FucK
fucA L-fuculose-phosphate aldolase FucA
tpi triose-phosphate isomerase AO353_05590 AO353_07945
aldA lactaldehyde dehydrogenase AO353_19510 AO353_06560
Alternative steps:
BPHYT_RS34240 ABC transporter for L-fucose, permease component
BPHYT_RS34245 ABC transporter for L-fucose, ATPase component AO353_20820 AO353_21385
BPHYT_RS34250 ABC transporter for L-fucose, substrate-binding component
fdh L-fucose 1-dehydrogenase AO353_01660
fucD L-fuconate dehydratase AO353_05290
fucDH 2-keto-3-deoxy-L-fuconate 4-dehydrogenase AO353_01660 AO353_28590
fucO L-lactaldehyde reductase AO353_17425 AO353_07245
fuconolactonase L-fucono-1,5-lactonase
fucP L-fucose:H+ symporter FucP AO353_25100
KDF-hydrolase 2,4-diketo-3-deoxy-L-fuconate hydrolase AO353_24305 AO353_08500
SM_b21103 ABC transporter for L-fucose, substrate-binding component
SM_b21104 ABC transporter for L-fucose, permease component 1
SM_b21105 ABC transporter for L-fucose, permease component 2 AO353_25890
SM_b21106 ABC transporter for L-fucose, ATPase component AO353_03380 AO353_25895

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory