Align NAD(P)+ L-lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized)
to candidate AO353_06560 AO353_06560 aldehyde dehydrogenase
Query= metacyc::MONOMER-16244 (495 letters) >FitnessBrowser__pseudo3_N2E3:AO353_06560 Length = 506 Score = 358 bits (918), Expect = e-103 Identities = 207/484 (42%), Positives = 285/484 (58%), Gaps = 17/484 (3%) Query: 16 TYEQPTGLFINNEFVQSKSKKTFGTVSPSTEEEITQVYEAFSEDIDDAVEAATAAFHSSW 75 +++ G +I EFV + F SP + I + + +EDI+ A++AA AA +W Sbjct: 14 SFKSKYGNYIGGEFVAPVKGQYFTNTSPVNGQPIAEFPRSTAEDIEKALDAAHAAA-DAW 72 Query: 76 STSDPQVRMKVLYKLADLIDEHADTLAHIEALDNGKSLMCS-KGDVALTAAYFRSCAGWT 134 ++ Q R VL K+AD I+++ + LA E+ DNGK++ + D+ L A +FR AG Sbjct: 73 GSTSAQARSLVLLKIADRIEQNLEVLAITESWDNGKAVRETLNADIPLAADHFRYFAGCL 132 Query: 135 DKIKGSVIETGDTHFNYTRREPIGVCGQIIPWNFPLLMASWKLGPVLCTGCTTVLKTAES 194 +GS E Y EP+GV GQIIPWNFP+LMA+WKL P L G VLK AE Sbjct: 133 RAQEGSAAEIDGNTVAYHIHEPLGVVGQIIPWNFPILMAAWKLAPALAAGNCVVLKPAEQ 192 Query: 195 TPLSALYLASLIKEAGAPPGVVNVVSGFGPTAGAPISSHPKIKKVAFTGSTATGRHIMKA 254 TPL L LI + PPGV+NVV GFG AG +++ +I K+AFTGST G HIMK Sbjct: 193 TPLGITVLLELIGDL-LPPGVLNVVQGFGKEAGEALATSKRIAKIAFTGSTPVGSHIMKC 251 Query: 255 AAESNLKKVTLELGGKSPNIVFDD------ADVKSTIQHLVTGIFYNTGEVCCAGSRIYV 308 AAE N+ T+ELGGKSPNI F D ++ + LV F+N GEVC SR V Sbjct: 252 AAE-NIIPSTVELGGKSPNIFFADIMQAEPTFIEKAAEGLVLA-FFNQGEVCTCPSRALV 309 Query: 309 QEGIYDKIVSEFKNAAESLKIGDPFKEDTFMGAQTSQLQLDKILKYIDIGKKEGATVITG 368 QE IYD + +K GDP DT +GAQ S+ Q DKIL Y++I K EGA ++TG Sbjct: 310 QESIYDDFMKVVMKKVLQIKRGDPLDTDTMVGAQASEQQFDKILSYLEIAKGEGAELLTG 369 Query: 369 GE---RFGN--KGYFIKPTIFGDVKEDHQIVRDEIFGPVVTITKFKTVEEVIALANDSEY 423 G+ GN GY+I+PT+ + ++ ++EIFGPVV+IT FK E +A+AND+E+ Sbjct: 370 GKVEKLEGNLATGYYIQPTLLKGTNK-MRVFQEEIFGPVVSITTFKDEAEALAIANDTEF 428 Query: 424 GLAAGVHTTNLSTAISVSNKINSGTIWVNTYNDFHPMVPFGGYSQSGIGREMGEEALDNY 483 GL AG+ T +++ A + I +G +W N Y+ + FGGY +SG+GRE + LD+Y Sbjct: 429 GLGAGLWTRDINRAYRMGRAIKAGRVWTNCYHLYPAHAAFGGYKKSGVGRETHKMMLDHY 488 Query: 484 TQVK 487 Q K Sbjct: 489 QQTK 492 Lambda K H 0.316 0.133 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 636 Number of extensions: 27 Number of successful extensions: 7 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 495 Length of database: 506 Length adjustment: 34 Effective length of query: 461 Effective length of database: 472 Effective search space: 217592 Effective search space used: 217592 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory