Align 2-dehydro-3-deoxygluconokinase; 2-keto-3-deoxygluconokinase; 3-deoxy-2-oxo-D-gluconate kinase; KDG kinase; EC 2.7.1.45 (characterized)
to candidate AO353_26875 AO353_26875 2-dehydro-3-deoxygluconokinase
Query= SwissProt::P50845 (324 letters) >FitnessBrowser__pseudo3_N2E3:AO353_26875 Length = 326 Score = 303 bits (777), Expect = 3e-87 Identities = 154/307 (50%), Positives = 213/307 (69%), Gaps = 3/307 (0%) Query: 2 KLDAVTFGESMAMFYANEYGGLHEVSTFSKGLAGAESNVACGLARLGFRMGWMSKVGNDQ 61 ++D ++FGE+MAMF A + G L +V+ F K +AGA+SNVA GL+RLGF++ W+S+VG D Sbjct: 3 EIDILSFGETMAMFVAEQSGDLADVTQFHKRIAGADSNVAIGLSRLGFKVSWLSRVGADS 62 Query: 62 LGTFILQELKKEGVDVSRVIRSQDENPTGLLLKSKVKEG-DPQVTYYRKNSAASTLTTAE 120 LG F++ L++EG+D R + + +PTG LKS+ +G DP V Y+R+ SAAS L+ Sbjct: 63 LGRFVVDTLEQEGLDC-RFVETDHAHPTGFQLKSRADDGSDPHVEYFRRGSAASLLSVQS 121 Query: 121 YPRDYFQCAGHLHVTGIPPALSAEMKDFTYHVMNDMRNAGKTISFDPNVRPSLWPDQATM 180 + + A HLH TGI PALS + ++ +M MR AG+++SFDPN+RP+LW + M Sbjct: 122 IVPEQLE-ARHLHATGIVPALSESARQMSFELMTRMRQAGRSVSFDPNLRPTLWGSEQQM 180 Query: 181 VHTINDLAGLADWFFPGIAEGELLTGEKTPEGIADYYLKKGASFVAIKLGKEGAYFKTGT 240 + IN LA LA W PG++EG LL+G + P IA +YL +GA V IKLG +GAY++T Sbjct: 181 IGEINRLAALAHWVLPGLSEGRLLSGFEDPADIAAFYLDQGAEAVVIKLGPDGAYYRTQH 240 Query: 241 SEGFLEGCRVDRVVDTVGAGDGFAVGVISGILDGLSYKDAVQRGNAIGALQVQAPGDMDG 300 +GF+ G V VVDTVGAGDGFAVG+IS +L+ LS DAVQR N IG+ VQ+ GDM+G Sbjct: 241 DQGFVPGVPVTTVVDTVGAGDGFAVGMISALLENLSVPDAVQRANWIGSRAVQSRGDMEG 300 Query: 301 LPTREKL 307 LP R +L Sbjct: 301 LPKRSEL 307 Lambda K H 0.317 0.135 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 316 Number of extensions: 17 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 324 Length of database: 326 Length adjustment: 28 Effective length of query: 296 Effective length of database: 298 Effective search space: 88208 Effective search space used: 88208 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory