Align fumarate hydratase (EC 4.2.1.2); (S)-2-methylmalate dehydratase (EC 4.2.1.34) (characterized)
to candidate AO353_03795 AO353_03795 fumarate hydratase
Query= BRENDA::Q141Z6 (520 letters) >FitnessBrowser__pseudo3_N2E3:AO353_03795 Length = 507 Score = 765 bits (1976), Expect = 0.0 Identities = 377/501 (75%), Positives = 431/501 (86%), Gaps = 1/501 (0%) Query: 14 MTVIKQEDLIQSIADSLQYISYYHPLDYIQALGRAYELEQSPAAKDAIAQILTNSRMCAE 73 MTVIKQ+DLIQS+AD+LQ+ISYYHP+D+IQA+ AY E+SPAA+D++AQIL NSRMCA Sbjct: 1 MTVIKQDDLIQSVADALQFISYYHPVDFIQAMHEAYLREESPAARDSMAQILINSRMCAT 60 Query: 74 GKRPICQDTGIVTVFVKVGMDVRWDGATMGVTDMINEGVRRGYLNPDNVLRASIVSPPEG 133 G RPICQDTGIVTVFV+VGMDVRWDGATM + DMINEGVRR Y P+NVLRASI++ P G Sbjct: 61 GHRPICQDTGIVTVFVRVGMDVRWDGATMSLDDMINEGVRRAYNLPENVLRASILADPAG 120 Query: 134 GRKNTKDNTPAVIHYEIVPGNTVDVQVAAKGGGSENKSKFAMLNPSDSIVDWILKTVPTM 193 RKNTKDNTPAVIHY IVPGNTV+V VAAKGGGSENKSK AMLNPSDSIVDW+LKTVP M Sbjct: 121 ARKNTKDNTPAVIHYSIVPGNTVEVDVAAKGGGSENKSKMAMLNPSDSIVDWVLKTVPEM 180 Query: 194 GAGWCPPGMLGIGIGGTAEKAMVMAKESLMDPIDIQDVIARGPKDWIEELRVELHEKVNA 253 GAGWCPPGMLGIGIGGTAEKA VMAKE LM+ IDI ++ RGP + IEE+R+EL EKVN Sbjct: 181 GAGWCPPGMLGIGIGGTAEKAAVMAKEVLMESIDIHELKKRGPSNRIEEMRLELFEKVNQ 240 Query: 254 LGIGAQGLGGLATVLDVKIMAAPTHAASKPVAIIPNCAATRHAHFTLDGSGAARLEAPSL 313 LGIGAQGLGGL TVLDVKIM PTHAAS PV +IPNCAATRHAHF LDGSG A LEAP L Sbjct: 241 LGIGAQGLGGLTTVLDVKIMDYPTHAASLPVCMIPNCAATRHAHFVLDGSGPASLEAPPL 300 Query: 314 DAWPKVHWQPDTEKSKRVDLNTLTPEEVAAWTPGQTLLLSGKMLTGRDAAHKRIADMLAK 373 DA+P++ W+ ++RV+L+TLTPEEV +W PG+T+LL+GKMLTGRDAAHKR+ +ML K Sbjct: 301 DAYPEIVWEAG-PSARRVNLDTLTPEEVQSWKPGETVLLNGKMLTGRDAAHKRMVEMLNK 359 Query: 374 GEKLPVDFTNRVIYYVGPVDPVRDEAVGPAGPTTATRMDKFTEMMLAQTGLISMIGKAER 433 GE LPVD R IYYVGPVDPVR+E VGPAGPTTATRMDKFT +L QTGL+ MIGK+ER Sbjct: 360 GETLPVDLKGRFIYYVGPVDPVREEVVGPAGPTTATRMDKFTRQILEQTGLLGMIGKSER 419 Query: 434 GPVAIEAIRKHKAAYLMAVGGAAYLVSKAIRSAKVLAFEDLGMEAIYEFDVQDMPVTVAV 493 GP AIEAI+ HKA YLMAVGGAAYLV++AI+ ++V+AF +LGMEAIYEFDV+DMPVTVAV Sbjct: 420 GPTAIEAIKDHKAVYLMAVGGAAYLVAQAIKKSRVVAFAELGMEAIYEFDVKDMPVTVAV 479 Query: 494 DSNGTSVHQTGPKEWQARIGK 514 DS G SVH TGP WQ +I + Sbjct: 480 DSKGESVHITGPAIWQKKISE 500 Lambda K H 0.316 0.133 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 922 Number of extensions: 29 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 520 Length of database: 507 Length adjustment: 35 Effective length of query: 485 Effective length of database: 472 Effective search space: 228920 Effective search space used: 228920 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory