Alignments for a candidate for glpK in Pseudomonas fluorescens FW300-N2E3

GapMind for catabolism of small carbon sources

Alignments for a candidate for glpK in Pseudomonas fluorescens FW300-N2E3

Align Glycerol kinase (EC 2.7.1.30) (characterized)
to candidate AO353_04180 AO353_04180 glycerol kinase

Query= reanno::WCS417:GFF1099
         (501 letters)



>FitnessBrowser__pseudo3_N2E3:AO353_04180
          Length = 501

 Score =  993 bits (2568), Expect = 0.0
 Identities = 486/501 (97%), Positives = 493/501 (98%)

Query: 1   MTDIQNKNYIIALDQGTTSSRAIIFDRDANVVCTAQREFAQHYPQAGWVEHDPMEIFATQ 60
           MTDIQNKNYIIALDQGTTSSRAIIFDRDANVVCTAQREFAQHYPQAGWVEHDPMEIFATQ
Sbjct: 1   MTDIQNKNYIIALDQGTTSSRAIIFDRDANVVCTAQREFAQHYPQAGWVEHDPMEIFATQ 60

Query: 61  SAVMVEALAQAGLHHDQVAAIGITNQRETTVVWDKVTGRPIYNAIVWQCRRSTEICQQLK 120
           SAVMVEALAQAGLHHDQVAAIGITNQRETTVVWDK TGRPIYNAIVWQCRRSTEICQQLK
Sbjct: 61  SAVMVEALAQAGLHHDQVAAIGITNQRETTVVWDKTTGRPIYNAIVWQCRRSTEICQQLK 120

Query: 121 RDGHEQYISDTTGLVTDPYFSGTKLKWILDNVEGSRERARNGELLFGTVDSWLIWKFTGG 180
           RDG EQYIS+TTGLVTDPYFSGTKLKWILDNVEGSRERAR GELLFGTVDSWLIWKFTGG
Sbjct: 121 RDGFEQYISETTGLVTDPYFSGTKLKWILDNVEGSRERARKGELLFGTVDSWLIWKFTGG 180

Query: 181 KTHVTDYTNASRTMLFNIHTLEWDAKMLEILDIPREMLPEVKSSSEIYGRTKSGIAIGGI 240
           KTHVTDYTNASRTMLFNIHTLEWDAKMLE+LDIPREMLPEVKSSSEIYG TKSGIAIGGI
Sbjct: 181 KTHVTDYTNASRTMLFNIHTLEWDAKMLEVLDIPREMLPEVKSSSEIYGHTKSGIAIGGI 240

Query: 241 AGDQQAALFGQMCVEAGQAKNTYGTGCFLLMNTGDKAVKSKHGMLTTIACGPRGEVAYAL 300
           AGDQQAALFGQMCVE GQAKNTYGTGCFLLMNTGDKAVKS+HGMLTTIACGPRGEVAYAL
Sbjct: 241 AGDQQAALFGQMCVEPGQAKNTYGTGCFLLMNTGDKAVKSRHGMLTTIACGPRGEVAYAL 300

Query: 301 EGAVFNGGSTVQWLRDELKIINDAHDTEYFAGKVKDSNGVYLVPAFTGLGAPYWDPYARG 360
           EGAVFNGGSTVQWLRDELKIINDAHDTEYFA KVKDSNGVYLVPAFTGLGAPYWDPYARG
Sbjct: 301 EGAVFNGGSTVQWLRDELKIINDAHDTEYFANKVKDSNGVYLVPAFTGLGAPYWDPYARG 360

Query: 361 ALFGLTRGVRVDHIIRAALESIAYQTRDVLDAMQQDSGERLKALRVDGGAVANNFLMQFQ 420
           ALFGLTRGVRVDHIIRAALESIAYQTRDVLDAMQ DSGERLKALRVDGGAVANNFLMQFQ
Sbjct: 361 ALFGLTRGVRVDHIIRAALESIAYQTRDVLDAMQHDSGERLKALRVDGGAVANNFLMQFQ 420

Query: 421 ADILGTQVERPQMRETTALGAAYLAGLACGFWGSLEELRGKAVIEREFEPQLDEAAKEKL 480
           ADILGT+VERPQMRETTALGAAYLAGLACGFWGSL+ELRGKAVIEREF+PQLDE AKEKL
Sbjct: 421 ADILGTKVERPQMRETTALGAAYLAGLACGFWGSLDELRGKAVIEREFDPQLDETAKEKL 480

Query: 481 YAGWQKAVSRTRDWEPHEGAE 501
           YAGW+KAVSRTRDWEPHEGAE
Sbjct: 481 YAGWKKAVSRTRDWEPHEGAE 501


Lambda     K      H
   0.319    0.134    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 920
Number of extensions: 14
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 501
Length of database: 501
Length adjustment: 34
Effective length of query: 467
Effective length of database: 467
Effective search space:   218089
Effective search space used:   218089
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

Align candidate AO353_04180 AO353_04180 (glycerol kinase)
to HMM TIGR01311 (glpK: glycerol kinase (EC 2.7.1.30))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR01311.hmm
# target sequence database:        /tmp/gapView.24971.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01311  [M=496]
Accession:   TIGR01311
Description: glycerol_kin: glycerol kinase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                     Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                     -----------
   2.8e-229  747.4   0.4   3.2e-229  747.2   0.4    1.0  1  lcl|FitnessBrowser__pseudo3_N2E3:AO353_04180  AO353_04180 glycerol kinase


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo3_N2E3:AO353_04180  AO353_04180 glycerol kinase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  747.2   0.4  3.2e-229  3.2e-229       1     496 []       8     494 ..       8     494 .. 0.99

  Alignments for each domain:
  == domain 1  score: 747.2 bits;  conditional E-value: 3.2e-229
                                     TIGR01311   1 kliaaiDqGttssraivfdkegeevakaqkelsqifpkegwvEhdpleilesvvkvlaealekle 65 
                                                   ++i+a+DqGttssrai+fd+++++v++aq+e+ q++p++gwvEhdp+ei++++ +v+ eal++++
  lcl|FitnessBrowser__pseudo3_N2E3:AO353_04180   8 NYIIALDQGTTSSRAIIFDRDANVVCTAQREFAQHYPQAGWVEHDPMEIFATQSAVMVEALAQAG 72 
                                                   59*************************************************************** PP

                                     TIGR01311  66 ikaeeiaaiGitnqREttvvWdketgkplvnaivWqdtrtakiveelkeetkeeelrektGLpls 130
                                                    + +++aaiGitnqREttvvWdk+tg+p++naivWq++r ++i+++lk+++ e++++e+tGL+ +
  lcl|FitnessBrowser__pseudo3_N2E3:AO353_04180  73 LHHDQVAAIGITNQRETTVVWDKTTGRPIYNAIVWQCRRSTEICQQLKRDGFEQYISETTGLVTD 137
                                                   ***************************************************************** PP

                                     TIGR01311 131 tYfsatKlrWlldnveevrkaaeegellfGtvdtwliykLtggkvhvtdvtNASRtlllnletlk 195
                                                   +Yfs+tKl+W+ldnve+ r++a++gellfGtvd+wli+k+tggk+hvtd+tNASRt+l+n++tl+
  lcl|FitnessBrowser__pseudo3_N2E3:AO353_04180 138 PYFSGTKLKWILDNVEGSRERARKGELLFGTVDSWLIWKFTGGKTHVTDYTNASRTMLFNIHTLE 202
                                                   ***************************************************************** PP

                                     TIGR01311 196 wdeellelfkipkellPeirsssevygeieekellkeevpitgvlGdqqaalvgqlclkkgeaKn 260
                                                   wd ++le+++ip+e+lPe++ssse+yg++++       + i g++Gdqqaal+gq+c+++g+aKn
  lcl|FitnessBrowser__pseudo3_N2E3:AO353_04180 203 WDAKMLEVLDIPREMLPEVKSSSEIYGHTKSG------IAIGGIAGDQQAALFGQMCVEPGQAKN 261
                                                   ****************************9987......*************************** PP

                                     TIGR01311 261 tYgtGcFlllntGekkviskhglLttvayklggkkptkyalEGsvavaGaavqwlrdnlklikka 325
                                                   tYgtGcFll+ntG+k+v s+hg+Ltt+a+   g+ +  yalEG+v+ +G++vqwlrd+lk+i++a
  lcl|FitnessBrowser__pseudo3_N2E3:AO353_04180 262 TYGTGCFLLMNTGDKAVKSRHGMLTTIACGPRGEVA--YALEGAVFNGGSTVQWLRDELKIINDA 324
                                                   *****************************9999886..*************************** PP

                                     TIGR01311 326 eeveklaksvedsegvyfVPafsGLfaPyWdsdArgtivGltrkttkehiaraaleavafqardi 390
                                                   +++e +a++v+ds+gvy+VPaf+GL+aPyWd+ Arg+++Gltr+++  hi+raale++a+q+rd+
  lcl|FitnessBrowser__pseudo3_N2E3:AO353_04180 325 HDTEYFANKVKDSNGVYLVPAFTGLGAPYWDPYARGALFGLTRGVRVDHIIRAALESIAYQTRDV 389
                                                   ***************************************************************** PP

                                     TIGR01311 391 leamekdagvevkvLkvDGglsknnllmqiqadilgvkverpkvaettalGaAlaaglavgvwks 455
                                                   l+am++d+g ++k+L+vDGg+++nn+lmq+qadilg+kverp++ ettalGaA++agla g+w s
  lcl|FitnessBrowser__pseudo3_N2E3:AO353_04180 390 LDAMQHDSGERLKALRVDGGAVANNFLMQFQADILGTKVERPQMRETTALGAAYLAGLACGFWGS 454
                                                   ***************************************************************** PP

                                     TIGR01311 456 eeeleksaeaeektfepemdeeerekkykkwkeaverslkw 496
                                                   ++el+ +a  e ++f p+ de+ +ek y+ wk+av+r+++w
  lcl|FitnessBrowser__pseudo3_N2E3:AO353_04180 455 LDELRGKAVIE-REFDPQLDETAKEKLYAGWKKAVSRTRDW 494
                                                   *****999997.9*************************998 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (496 nodes)
Target sequences:                          1  (501 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.03
# Mc/sec: 7.31
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources