Align Methionine import ATP-binding protein MetN 2, component of L-Histidine uptake porter, MetIQN (characterized)
to candidate AO353_10890 AO353_10890 methionine ABC transporter ATP-binding protein
Query= TCDB::Q9HT70 (335 letters) >FitnessBrowser__pseudo3_N2E3:AO353_10890 Length = 335 Score = 546 bits (1407), Expect = e-160 Identities = 271/335 (80%), Positives = 308/335 (91%) Query: 1 MIEFHDVHKTYRVAGREIPALQPTRLNIQAGQIFGLIGHSGAGKSTLLRLINRLEEPSGG 60 +IEF +VHKTYRVAG++IPAL PT L+I+ GQ++GLIGHSGAGKSTLLRLINRLE+ SGG Sbjct: 1 VIEFQNVHKTYRVAGKDIPALHPTNLSIENGQVYGLIGHSGAGKSTLLRLINRLEDSSGG 60 Query: 61 RILVEGEDVTALDAEGLRRFRQRVGMIFQHFNLLSSKTVADNIAMPLRLAGGFSRAEVDA 120 +I+V+GE+VTALDA LRRFRQ+VGMIFQHFNLL+SKTVADN+A+PL LAG SR+E+D Sbjct: 61 KIIVDGEEVTALDANALRRFRQQVGMIFQHFNLLASKTVADNVALPLTLAGELSRSEIDQ 120 Query: 121 RVSELLARVGLSDHARKYPAQLSGGQKQRVGIARALACRPSILLCDEATSALDPQTTASV 180 RV+ELLARVGLSDHA+KYPAQLSGGQKQRVGIARALA +P ILLCDEATSALDPQTTASV Sbjct: 121 RVAELLARVGLSDHAKKYPAQLSGGQKQRVGIARALATKPKILLCDEATSALDPQTTASV 180 Query: 181 LQLLAEINRELKLTIVLITHEMDVIRRVCDQVAVMDGGAIVEQGDVADVFLHPQHPTTRR 240 LQLLAEINREL LTIVLITHEMDVIRRVCDQVAVMD G IVEQG VADVFLHP+HPTT+R Sbjct: 181 LQLLAEINRELNLTIVLITHEMDVIRRVCDQVAVMDAGVIVEQGPVADVFLHPKHPTTKR 240 Query: 241 FVFEAERVDEDERHDDFAHVPGLILRLTFRGEATYAPLLGTVARQTGVDYSILSGRIDRI 300 FV E E++DE E+ DDFAHVPG I+RLTF+G+ATYAPLLGTVAR+TGVDYSIL+GRIDRI Sbjct: 241 FVQEDEQIDESEQRDDFAHVPGRIVRLTFQGDATYAPLLGTVARETGVDYSILAGRIDRI 300 Query: 301 KDTPYGQLTLALVGGDLEAAMSQLNAADVHVEVLR 335 KD PYGQLTLA+ GGD+EAA ++ AADVH+EVLR Sbjct: 301 KDVPYGQLTLAVTGGDMEAAFARFTAADVHMEVLR 335 Lambda K H 0.322 0.138 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 468 Number of extensions: 11 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 335 Length of database: 335 Length adjustment: 28 Effective length of query: 307 Effective length of database: 307 Effective search space: 94249 Effective search space used: 94249 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 49 (23.5 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory