GapMind for catabolism of small carbon sources

 

Finding step hisJ for L-histidine catabolism in Pseudomonas fluorescens FW300-N2E3

5 candidates for hisJ: L-histidine ABC transporter, substrate-binding component HisJ

Score Gene Description Similar to Id. Cov. Bits Other hit Other id. Other bits
hi AO353_11995 amino acid ABC transporter Amino acid (Lysine/arginine/ornithine/histidine/octopine) ABC transporter periplasmic binding protein, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR (characterized) 80% 100% 412.9 AotJ aka PA0888, component of Arginine/ornithine (but not lysine) porter 44% 209.5
hi AO353_29015 ABC transporter substrate-binding protein histidine ABC transporter, periplasmic histidine-binding protein HisJ (characterized) 52% 100% 283.5 ArgT aka B2310, component of Histidine/Arginine/Lysine (basic amino acid) uptake porter, HisJ/ArgT/HisP/HisM/HisQ [R, R, C, M, M, respectively] (Gilson et al. 1982). HisJ binds L-His (preferred), but 1-methyl-L-His and 3-methyl-L-His also bind, while the dipeptide carnosine binds weakly; D-histidine and the histidine degradation products, histamine, urocanic acid and imidazole do not bind. L-Arg, homo-L-Arg, and post-translationally modified methylated Arg-analogs also bind with the exception of symmetric dimethylated-L-Arg. L-Lys and L-Orn show weaker interactions with HisJ and methylated and acetylated Lys variants show poor binding.The carboxylate groups of these amino acids and their variants are essential 48% 262.3
med AO353_28165 ABC transporter substrate-binding protein histidine ABC transporter, periplasmic histidine-binding protein HisJ (characterized) 47% 96% 249.2 ABC transporter for L-Arginine, periplasmic substrate-binding component 53% 270.4
med AO353_04645 ABC transporter substrate-binding protein histidine ABC transporter, periplasmic histidine-binding protein HisJ (characterized) 44% 100% 222.6 AotJ aka PA0888, component of Arginine/ornithine (but not lysine) porter 62% 324.3
med AO353_03055 ABC transporter substrate-binding protein histidine ABC transporter, periplasmic histidine-binding protein HisJ (characterized) 42% 100% 212.6 ABC transporter for L-Arginine and L-Citrulline, periplasmic substrate-binding component 100% 511.9

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

GapMind searches the predicted proteins for candidates by using ublast (a fast alternative to protein BLAST) to find similarities to characterized proteins or by using HMMer to find similarities to enzyme models (usually from TIGRFams). For alignments to characterized proteins (from ublast), scores of 44 bits correspond to an expectation value (E) of about 0.001.

Also see fitness data for the candidates

Definition of step hisJ

Or cluster all characterized hisJ proteins

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory