GapMind for catabolism of small carbon sources

 

Aligments for a candidate for fadB in Pseudomonas fluorescens FW300-N2E3

Align Peroxisomal multifunctional enzyme A; MFE-A; MFE-1; EC 1.1.1.35 (characterized)
to candidate AO353_15525 AO353_15525 serine/threonine protein kinase

Query= SwissProt::Q9NKW1
         (441 letters)



>lcl|FitnessBrowser__pseudo3_N2E3:AO353_15525 AO353_15525
           serine/threonine protein kinase
          Length = 303

 Score =  350 bits (899), Expect = e-101
 Identities = 163/299 (54%), Positives = 228/299 (76%), Gaps = 3/299 (1%)

Query: 2   ALNFKDKVVIVTGAGGGIGKVYALEFAKRGAKVVVNDLGGSHTGQGSSSKAADKVVEEIK 61
           ++ F+DKVVIVTGAGGG+G+ +AL FAK+GAKV+VNDLGGS  G+G+++ AAD+VV EI+
Sbjct: 4   SVRFEDKVVIVTGAGGGLGRAHALLFAKQGAKVLVNDLGGSAQGEGANASAADRVVAEIR 63

Query: 62  AAGGTAVANYDSVEDGEKIVQTAMDSFGGVDILINNAGILRDVSFGKMTDGDWDLVYRVH 121
            AGG+AVAN+DSV DG+K+VQ A+D+FG +D+++NNAGILRD +F KM DGDWDLVYRVH
Sbjct: 64  EAGGSAVANHDSVTDGDKLVQHALDTFGRIDVVVNNAGILRDKTFHKMEDGDWDLVYRVH 123

Query: 122 AKGAYKLSRAAWNHMREKNFGRIIMTSSAAGLYGNFGQANYGSMKMALVGLSNTLAQEGK 181
            +GAYK++RAAW HMRE+N+GR+I T+S +G+YGNFGQ+NYG  K+ L GL+ TLA EG+
Sbjct: 124 VEGAYKVTRAAWPHMREQNYGRVIFTASTSGIYGNFGQSNYGMAKLGLYGLTRTLALEGR 183

Query: 182 SKNIHCNTIAPIAASRLTESVMPPEILEQMKPDYIVPLVLYLCHQDTTETGGVFEVGAGW 241
             NI  N IAP   +R+TE ++PP++ EQ+KP+ + PLV+YL  ++  ET G+FEVG GW
Sbjct: 184 KNNILVNAIAPTGGTRMTEGLIPPQVFEQLKPELVSPLVVYLASENCQETSGLFEVGGGW 243

Query: 242 VSKVRLQRSAGVYM---KDLTPEKIKDNWAQIESFDNPSYPTSASESVSGILAAVNSKP 297
           + KVR +RS G      +  +PE +  +W QI  F+  ++P    E++  ++A +   P
Sbjct: 244 MGKVRWERSLGAGFDPREGFSPEDVAAHWEQICDFEGAAHPKDNIEALKEMMANLQKYP 302


Lambda     K      H
   0.313    0.131    0.371 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 419
Number of extensions: 21
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 441
Length of database: 303
Length adjustment: 30
Effective length of query: 411
Effective length of database: 273
Effective search space:   112203
Effective search space used:   112203
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory