GapMind for catabolism of small carbon sources

 

Alignments for a candidate for hglS in Pseudomonas fluorescens FW300-N2E3

Align putative hydrolase, required for lysine catabolism (characterized)
to candidate AO353_21295 AO353_21295 hypothetical protein

Query= reanno::pseudo5_N2C3_1:AO356_01105
         (458 letters)



>FitnessBrowser__pseudo3_N2E3:AO353_21295
          Length = 465

 Score =  727 bits (1877), Expect = 0.0
 Identities = 372/455 (81%), Positives = 403/455 (88%)

Query: 4   VSPDLIRQRFSRAMSDMYRDEVPLYGALMKLVEHTNAQVLSEQPQLAEHLRSTGELERLD 63
           VSPDLIRQRFSRAMSDMYR+EVPLYGALM+LV  TN QVL   P +A  LRSTGE+ERLD
Sbjct: 7   VSPDLIRQRFSRAMSDMYREEVPLYGALMELVAQTNVQVLDSDPAIARQLRSTGEIERLD 66

Query: 64  LERHGAIRVGTAAELATLGRLFAVMGMQPVGYYDLTPAGVPVHSTAFRAVHESALQVSPF 123
           LERHGAIRVGTA ELATL RLFAVMGMQPVGYYDLTPAGVPVHSTAFRAVHE++LQVSPF
Sbjct: 67  LERHGAIRVGTAQELATLCRLFAVMGMQPVGYYDLTPAGVPVHSTAFRAVHEASLQVSPF 126

Query: 124 RVFTSLLRLELIENSELRAFAESVLAKRQIFTPGALALIDLAERQGGLTQAQAEDFVLQA 183
           RVFTSLLRLELIEN ELRAFAESVLAKR IFT  AL LI+ AE+ GGL + QAEDFV QA
Sbjct: 127 RVFTSLLRLELIENPELRAFAESVLAKRSIFTAQALKLIEQAEQHGGLDEQQAEDFVEQA 186

Query: 184 LETFRWHHSATVTAEQYRQLSAQHRLIADVVAFKGPHINHLTPRTLDIDIVQARMPVHGI 243
           LETFRWHH ATVTA QY+QLSAQHRLIADVVAFKGPHINHLTPRTLDIDIVQA+MP +GI
Sbjct: 187 LETFRWHHRATVTAAQYQQLSAQHRLIADVVAFKGPHINHLTPRTLDIDIVQAKMPEYGI 246

Query: 244 TPKAVIEGPPRRQCPILLRQTSFKALDEPVAFTDQPQAQGSHSARFGEIEQRGAALTPKG 303
           TPKAVIEGPPRRQCPILLRQTSFKALDEP+AFTDQ QA GSHSARFGEIEQRGAALTP+G
Sbjct: 247 TPKAVIEGPPRRQCPILLRQTSFKALDEPIAFTDQQQALGSHSARFGEIEQRGAALTPEG 306

Query: 304 RALYDQLLNAARDALGAFPNEANAERYNALMTEHFVAFPDNHDELRQQALAYFRYFPTPK 363
           RALYD+LLNAARDAL  FPNEANA RY++LM +HF AFPD+++E+R+Q LAYFRYF T K
Sbjct: 307 RALYDRLLNAARDALNDFPNEANAARYHSLMEQHFKAFPDSYNEMREQGLAYFRYFVTEK 366

Query: 364 GLAAKGTLEPAASLEHLLEQQYLRAEPLVYEDFLPVSAAGIFQSNLGDAAQSHYAGQSNR 423
           GLAA      +ASL  LL+ +Y+RA PLVYEDFLPVSAAGIFQSNLGDAAQ+HY   SNR
Sbjct: 367 GLAADSDELGSASLRGLLDAEYIRAVPLVYEDFLPVSAAGIFQSNLGDAAQTHYGEHSNR 426

Query: 424 QAFEAALGRATIDELGLYAQTQQRSIDECRAALGV 458
           QAFE ALGR+TIDEL LYA TQ+RSI+EC AAL V
Sbjct: 427 QAFEQALGRSTIDELALYADTQRRSIEECCAALNV 461


Lambda     K      H
   0.320    0.134    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 778
Number of extensions: 18
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 458
Length of database: 465
Length adjustment: 33
Effective length of query: 425
Effective length of database: 432
Effective search space:   183600
Effective search space used:   183600
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory