Align ABC transporter for D-Maltose and D-Trehalose, ATPase component (characterized)
to candidate AO353_25130 AO353_25130 ABC transporter
Query= reanno::Smeli:SMc03065 (362 letters) >FitnessBrowser__pseudo3_N2E3:AO353_25130 Length = 381 Score = 340 bits (872), Expect = 4e-98 Identities = 189/367 (51%), Positives = 246/367 (67%), Gaps = 15/367 (4%) Query: 1 MTGLLLKDIRKSYGAVDVIHGIDLDIKEGEFVVFVGPSGCGKSTLLRMIAGLEEITGGDM 60 M L L ++ K G V ++ + L+I GEFVVFVGPSGCGKSTLLR+IAGL+ I GD+ Sbjct: 1 MIKLKLDNVNKQLGGVRILRDVSLEIAAGEFVVFVGPSGCGKSTLLRLIAGLDSICAGDL 60 Query: 61 FIDGERVNDVPPSKRGIAMVFQSYALYPHMTVYDNMAFGMRIARESKEEIDRRVRGAADM 120 ID RVND+ P +RG+ MVFQSYALYPHM+VYDN++FG+++A+ K + RV A + Sbjct: 61 LIDERRVNDLEPRERGVGMVFQSYALYPHMSVYDNISFGLKLAKTEKSSLRERVLRTAQI 120 Query: 121 LQLTPYLDRLPKALSGGQRQRVAIGRAICRNPKVFLFDEPLSNLDAALRVATRIEIAKLS 180 LQL L R PK LSGGQRQRVA+GRA+ R P + LFDEPLSNLDA+LRV R EIA+L Sbjct: 121 LQLDKLLQRKPKELSGGQRQRVAMGRAMAREPDILLFDEPLSNLDASLRVQMRNEIARLH 180 Query: 181 ERMSDTTMIYVTHDQVEAMTLADRIVVLSAGHIEQVGAPLELYERPANLFVARFIGSPAM 240 R+ +TMIYVTHDQVEAMTLAD+IVVL+ G +EQVG+P ELYERPA+ FVA F+GSP M Sbjct: 181 ARLG-STMIYVTHDQVEAMTLADKIVVLNGGRVEQVGSPRELYERPASRFVAGFLGSPRM 239 Query: 241 NVIPATITATGQQTAVS--LAGGKSVTLDVPTNASENGKTASFGVRPEDLRVTEADDFLF 298 N + A + A G+ + V + G S+ D A++ S GVRPE + + AD Sbjct: 240 NFLAARLHAPGETSLVDTPVLGMTSLPFDSSNLAADT--PLSLGVRPEHVSLKAAD---- 293 Query: 299 EGTVSI----VEALGEVTLLYIEGLVENEPIIAKMPGIARVGRGDKVRFTADKAKLHLFD 354 GTV + VE LG T ++++ +++P+I + A GD+V D LHLFD Sbjct: 294 -GTVGVIVTGVEYLGSETYVHLD-TGQDDPLICRCEVNAGWQVGDRVELQLDIGNLHLFD 351 Query: 355 TNGQSYR 361 +G + R Sbjct: 352 ADGTALR 358 Lambda K H 0.320 0.137 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 368 Number of extensions: 14 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 362 Length of database: 381 Length adjustment: 30 Effective length of query: 332 Effective length of database: 351 Effective search space: 116532 Effective search space used: 116532 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 49 (23.5 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory