GapMind for catabolism of small carbon sources


Alignments for a candidate for cmtB in Pseudomonas fluorescens FW300-N2E3

Align Mannitol-specific phosphotransferase enzyme IIA component (characterized, see rationale)
to candidate AO353_05485 AO353_05485 PTS fructose transporter subunit IIA

Query= uniprot:Q45420
         (145 letters)

          Length = 953

 Score =  104 bits (260), Expect = 3e-27
 Identities = 52/133 (39%), Positives = 81/133 (60%)

           E I +     +K  A+ L    LVN+G V + Y+  +  REA  ST++G  IAIPHGT D

            +  V  +G+ ++Q P+GVD+GDG+IV L IGIA K++EHL +L  +     E +  + +

Query: 129 IKAATEEEILSIL 141
            +A++ E +L +L
Sbjct: 127 RRASSAEALLKLL 139

Lambda     K      H
   0.316    0.135    0.365 

Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 241
Number of extensions: 12
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 145
Length of database: 953
Length adjustment: 29
Effective length of query: 116
Effective length of database: 924
Effective search space:   107184
Effective search space used:   107184
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.



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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory