Align mannitol 2-dehydrogenase (EC 1.1.1.67) (characterized)
to candidate AO353_25900 AO353_25900 mannitol dehydrogenase
Query= BRENDA::O08355 (493 letters) >FitnessBrowser__pseudo3_N2E3:AO353_25900 Length = 491 Score = 874 bits (2259), Expect = 0.0 Identities = 427/489 (87%), Positives = 457/489 (93%) Query: 1 MKLNKQNLTQLAPEVKLPAYTLADTRQGIAHIGVGGFHRAHQAYYTDALMNTGEGLDWSI 60 MKLNK NLT+LAPEV LPAYTL+ TRQGIAHIGVGGFHRAHQAYYTDALMNTGEGLDWSI Sbjct: 1 MKLNKPNLTRLAPEVALPAYTLSATRQGIAHIGVGGFHRAHQAYYTDALMNTGEGLDWSI 60 Query: 61 CGVGLRSEDRKARDDLAGQDYLFTLYELGDTDDTEVRVIGSISDMLLAEDSAQALIDKLA 120 CG+GLR+EDR+ARDDLA QDYLFTL+ELGD+ DTEVRVIG+ISDMLLAED QALIDKLA Sbjct: 61 CGIGLRAEDRRARDDLASQDYLFTLFELGDSGDTEVRVIGAISDMLLAEDDTQALIDKLA 120 Query: 121 SPEIRIVSLTITEGGYCIDDSNGEFMAHLPQIQHDLAHPSSPKTVFGFICAALTQRRAAG 180 SP+IRIVSLTITEGGYCIDDS GEFMA LPQIQHDLAHP++PKTVFGF+CAAL +RRAAG Sbjct: 121 SPDIRIVSLTITEGGYCIDDSTGEFMAQLPQIQHDLAHPNAPKTVFGFLCAALAKRRAAG 180 Query: 181 IPAFTVMSCDNLPHNGAVTRKALLAFAALHNAELHDWIKAHVSFPNAMVDRITPMTSTAH 240 IPAFT+MSCDNLPHNGAVTRKALLAFAAL +A+L DWI A+VSFPNAMVDRITPMTS+AH Sbjct: 181 IPAFTLMSCDNLPHNGAVTRKALLAFAALRDADLRDWIGANVSFPNAMVDRITPMTSSAH 240 Query: 241 RLQLHDEHGIDDAWPVVCEPFVQWVLEDKFVNGRPAWEKVGVQFTDDVTPYEEMKIGLLN 300 RL LHDEHGIDD WPVVCEPFVQWVLED FVNGRPAWEKVGVQFTDDVTPYEEMKI LLN Sbjct: 241 RLHLHDEHGIDDLWPVVCEPFVQWVLEDNFVNGRPAWEKVGVQFTDDVTPYEEMKIKLLN 300 Query: 301 GSHLALTYLGFLKGYRFVHETMNDPLFVAYMRAYMDLDVTPNLAPVPGIDLTDYKQTLVD 360 GSHLALTYLGFLKGYRFVHETMNDPLFV YMRAYMDLDVTP LAPVPGIDLT YK TLV+ Sbjct: 301 GSHLALTYLGFLKGYRFVHETMNDPLFVRYMRAYMDLDVTPQLAPVPGIDLTQYKNTLVE 360 Query: 361 RFSNQAIADQLERVCSDGSSKFPKFTVPTINRLIADGRETERAALVVAAWALYLKGVDEN 420 RFSNQAIADQL RVCSDGSSKFPKFTVPTINRLIADGRET+RAALVVAAWA+YLKGVDEN Sbjct: 361 RFSNQAIADQLARVCSDGSSKFPKFTVPTINRLIADGRETKRAALVVAAWAIYLKGVDEN 420 Query: 421 GVSYTIPDPRAEFCQGLVSDDALISQRLLAVEEIFGTAIPNSPEFVAAFERCYGSLRDNG 480 G +Y IPDPRAEFCQ LV+DDALI+QR+L VEEIFGTAIP+S EFVAAFE C SLR++G Sbjct: 421 GDTYPIPDPRAEFCQALVADDALITQRMLEVEEIFGTAIPHSAEFVAAFEWCCNSLREDG 480 Query: 481 VTTTLKHLL 489 VT TL+ +L Sbjct: 481 VTRTLEKIL 489 Lambda K H 0.321 0.137 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 938 Number of extensions: 32 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 493 Length of database: 491 Length adjustment: 34 Effective length of query: 459 Effective length of database: 457 Effective search space: 209763 Effective search space used: 209763 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory