GapMind for catabolism of small carbon sources

 

L-phenylalanine catabolism in Pseudomonas fluorescens FW300-N2E3

Best path

livF, livG, livH, livM, livJ, PAH, PCBD, QDPR, HPD, hmgA, maiA, fahA, atoA, atoD, atoB

Also see fitness data for the top candidates

Rules

Overview: Phenylalanine utilization in GapMind is based on MetaCyc pathway L-phenylalanine degradation I (aerobic, via tyrosine, link), pathway II (anaerobic, via phenylacetaldehyde dehydrogenase, link), degradation via phenylpyruvate:ferredoxin oxidoreductase (PMC3346364), or degradation via phenylacetaldehyde:ferredoxin oxidoreductase (PMID:24214948). (MetaCyc describes additional pathways, but they do not result in carbon incorporation or are not reported in prokaryotes, so they are not included in GapMind.)

76 steps (43 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
livF L-phenylalanine ABC transporter, ATPase component 1 (LivF) AO353_17105 AO353_13360
livG L-phenylalanine ABC transporter, ATPase component 2 (LivG) AO353_17110 AO353_13355
livH L-phenylalanine ABC transporter, permease component 1 (LivH) AO353_17120 AO353_13345
livM L-phenylalanine ABC transporter, permease component 2 (LivM) AO353_17115 AO353_13350
livJ L-phenylalanine ABC transporter, substrate-binding component LivJ/LivK AO353_17125 AO353_13340
PAH phenylalanine 4-monooxygenase AO353_18585
PCBD pterin-4-alpha-carbinoalamine dehydratase AO353_18580
QDPR 6,7-dihydropteridine reductase AO353_04955 AO353_15440
HPD 4-hydroxyphenylpyruvate dioxygenase AO353_26925 AO353_06185
hmgA homogentisate dioxygenase AO353_15550
maiA maleylacetoacetate isomerase AO353_15560 AO353_10285
fahA fumarylacetoacetate hydrolase AO353_15555 AO353_24305
atoA acetoacetyl-CoA transferase, A subunit AO353_27935
atoD acetoacetyl-CoA transferase, B subunit AO353_27940
atoB acetyl-CoA C-acetyltransferase AO353_25685 AO353_27945
Alternative steps:
aacS acetoacetyl-CoA synthetase AO353_21765 AO353_20355
ARO10 phenylpyruvate decarboxylase
ARO8 L-phenylalanine transaminase AO353_18575 AO353_20540
aroP L-phenylalanine:H+ symporter AroP AO353_05965 AO353_18555
badH 2-hydroxy-cyclohexanecarboxyl-CoA dehydrogenase AO353_04045 AO353_01660
badI 2-ketocyclohexanecarboxyl-CoA hydrolase AO353_25675 AO353_25665
badK cyclohex-1-ene-1-carboxyl-CoA hydratase AO353_25675 AO353_03780
bamB class II benzoyl-CoA reductase, BamB subunit
bamC class II benzoyl-CoA reductase, BamC subunit
bamD class II benzoyl-CoA reductase, BamD subunit AO353_07705
bamE class II benzoyl-CoA reductase, BamE subunit
bamF class II benzoyl-CoA reductase, BamF subunit
bamG class II benzoyl-CoA reductase, BamG subunit
bamH class II benzoyl-CoA reductase, BamH subunit AO353_15695 AO353_27760
bamI class II benzoyl-CoA reductase, BamI subunit AO353_15690
bcrA ATP-dependent benzoyl-CoA reductase, alpha subunit
bcrB ATP-dependent benzoyl-CoA reductase, beta subunit
bcrC ATP-dependent benzoyl-CoA reductase, gamma subunit
bcrD ATP-dependent benzoyl-CoA reductase, delta subunit
boxA benzoyl-CoA epoxidase, subunit A
boxB benzoyl-CoA epoxidase, subunit B
boxC 2,3-epoxybenzoyl-CoA dihydrolase
boxD 3,4-dehydroadipyl-CoA semialdehyde dehydrogenase
Ch1CoA cyclohex-1-ene-1-carbonyl-CoA dehydrogenase AO353_25680 AO353_20350
dch cyclohexa-1,5-diene-1-carboxyl-CoA hydratase AO353_25675 AO353_00510
ech (S)-3-hydroxybutanoyl-CoA hydro-lyase AO353_25675 AO353_00510
fadB (S)-3-hydroxybutanoyl-CoA dehydrogenase AO353_00510 AO353_27535
gcdH glutaryl-CoA dehydrogenase AO353_11100 AO353_20350
had 6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase AO353_19865 AO353_07635
iorA phenylpyruvate:ferredoxin oxidoreductase, IorA subunit
iorAB phenylpyruvate:ferredoxin oxidoreductase, fused IorA/IorB
iorB phenylpyruvate:ferredoxin oxidoreductase, IorB subunit
oah 6-oxocyclohex-1-ene-1-carbonyl-CoA hydratase AO353_25675
paaA phenylacetyl-CoA 1,2-epoxidase, subunit A
paaB phenylacetyl-CoA 1,2-epoxidase, subunit B
paaC phenylacetyl-CoA 1,2-epoxidase, subunit C
paaE phenylacetyl-CoA 1,2-epoxidase, subunit E
paaF 2,3-dehydroadipyl-CoA hydratase AO353_25675 AO353_24830
paaG 1,2-epoxyphenylacetyl-CoA isomerase / 2-(oxepinyl)acetyl-CoA isomerase / didehydroadipyl-CoA isomerase AO353_24830 AO353_25675
paaH 3-hydroxyadipyl-CoA dehydrogenase AO353_00510 AO353_27535
paaJ1 3-oxo-5,6-dehydrosuberyl-CoA thiolase AO353_17205 AO353_01065
paaJ2 3-oxoadipyl-CoA thiolase AO353_17205 AO353_01065
paaK phenylacetate-CoA ligase AO353_03315 AO353_03310
paaZ1 oxepin-CoA hydrolase AO353_25675 AO353_03780
paaZ2 3-oxo-5,6-didehydrosuberyl-CoA semialdehyde dehydrogenase
pad-dh phenylacetaldehyde dehydrogenase AO353_19510 AO353_09195
padB phenylacetyl-CoA dehydrogenase, PadB subunit
padC phenylacetyl-CoA dehydrogenase, PadC subunit
padD phenylacetyl-CoA dehydrogenase, PadD subunit
padE phenylglyoxylate dehydrogenase, gamma subunit
padF phenylglyoxylate dehydrogenase, delta subunit
padG phenylglyoxylate dehydrogenase, alpha subunit
padH phenylglyoxylate dehydrogenase, epsilon subunit
padI phenylglyoxylate dehydrogenase, beta subunit
pfor phenylacetaldeyde:ferredoxin oxidoreductase
pimB 3-oxopimeloyl-CoA:CoA acetyltransferase AO353_17205 AO353_01065
pimC pimeloyl-CoA dehydrogenase, small subunit
pimD pimeloyl-CoA dehydrogenase, large subunit
pimF 6-carboxyhex-2-enoyl-CoA hydratase AO353_00510
PPDCalpha phenylpyruvate decarboxylase, alpha subunit AO353_15575 AO353_26635
PPDCbeta phenylpyruvate decarboxylase, beta subunit AO353_15580 AO353_26640

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory