Alignments for a candidate for HPD in Pseudomonas fluorescens FW300-N2E3

GapMind for catabolism of small carbon sources

Alignments for a candidate for HPD in Pseudomonas fluorescens FW300-N2E3

Align 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27) (characterized)
to candidate AO353_26925 AO353_26925 4-hydroxyphenylpyruvate dioxygenase

Query= reanno::pseudo3_N2E3:AO353_26925
         (358 letters)



>FitnessBrowser__pseudo3_N2E3:AO353_26925
          Length = 358

 Score =  728 bits (1880), Expect = 0.0
 Identities = 358/358 (100%), Positives = 358/358 (100%)

Query: 1   MADLYENPMGLMGFEFIELASPTPNTLEPIFEIMGFTKVATHRSKDVHLYRQGQINLILN 60
           MADLYENPMGLMGFEFIELASPTPNTLEPIFEIMGFTKVATHRSKDVHLYRQGQINLILN
Sbjct: 1   MADLYENPMGLMGFEFIELASPTPNTLEPIFEIMGFTKVATHRSKDVHLYRQGQINLILN 60

Query: 61  NEPHSVASYFAAEHGPSVCGMAFRVKDSQKAYKRALELGAQPIHIETGPMELNLPAIKGI 120
           NEPHSVASYFAAEHGPSVCGMAFRVKDSQKAYKRALELGAQPIHIETGPMELNLPAIKGI
Sbjct: 61  NEPHSVASYFAAEHGPSVCGMAFRVKDSQKAYKRALELGAQPIHIETGPMELNLPAIKGI 120

Query: 121 GGAPLYLIDRFGEGSSIYDIDFVFIEGVDRHPVGAGLKIIDHLTHNVYRGRMAYWANFYE 180
           GGAPLYLIDRFGEGSSIYDIDFVFIEGVDRHPVGAGLKIIDHLTHNVYRGRMAYWANFYE
Sbjct: 121 GGAPLYLIDRFGEGSSIYDIDFVFIEGVDRHPVGAGLKIIDHLTHNVYRGRMAYWANFYE 180

Query: 181 KLFNFREIRYFDIKGEYTGLTSKAMTAPDGMIRIPLNEESSKGAGQIEEFLMQFNGEGIQ 240
           KLFNFREIRYFDIKGEYTGLTSKAMTAPDGMIRIPLNEESSKGAGQIEEFLMQFNGEGIQ
Sbjct: 181 KLFNFREIRYFDIKGEYTGLTSKAMTAPDGMIRIPLNEESSKGAGQIEEFLMQFNGEGIQ 240

Query: 241 HVAFLSDDLIKTWDHLKKIGMRFMTAPPDTYYEMLEGRLPNHGEPVNELQARGILLDGSS 300
           HVAFLSDDLIKTWDHLKKIGMRFMTAPPDTYYEMLEGRLPNHGEPVNELQARGILLDGSS
Sbjct: 241 HVAFLSDDLIKTWDHLKKIGMRFMTAPPDTYYEMLEGRLPNHGEPVNELQARGILLDGSS 300

Query: 301 EAGDKRLLLQIFSETLMGPVFFEFIQRKGDDGFGEGNFKALFESIERDQVRRGVLSTE 358
           EAGDKRLLLQIFSETLMGPVFFEFIQRKGDDGFGEGNFKALFESIERDQVRRGVLSTE
Sbjct: 301 EAGDKRLLLQIFSETLMGPVFFEFIQRKGDDGFGEGNFKALFESIERDQVRRGVLSTE 358


Lambda     K      H
   0.321    0.141    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 690
Number of extensions: 21
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 358
Length of database: 358
Length adjustment: 29
Effective length of query: 329
Effective length of database: 329
Effective search space:   108241
Effective search space used:   108241
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

Align candidate AO353_26925 AO353_26925 (4-hydroxyphenylpyruvate dioxygenase)
to HMM TIGR01263 (hppD: 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR01263.hmm
# target sequence database:        /tmp/gapView.12864.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01263  [M=353]
Accession:   TIGR01263
Description: 4HPPD: 4-hydroxyphenylpyruvate dioxygenase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                     Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                     -----------
   1.1e-129  418.9   0.0   1.3e-129  418.7   0.0    1.0  1  lcl|FitnessBrowser__pseudo3_N2E3:AO353_26925  AO353_26925 4-hydroxyphenylpyruv


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo3_N2E3:AO353_26925  AO353_26925 4-hydroxyphenylpyruvate dioxygenase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  418.7   0.0  1.3e-129  1.3e-129       1     353 []      12     355 ..      12     355 .. 0.98

  Alignments for each domain:
  == domain 1  score: 418.7 bits;  conditional E-value: 1.3e-129
                                     TIGR01263   1 kgfdfvefavgdakqaakalveklGfeavaketgsrekastvlrqgeitlvltaelsssseaaaf 65 
                                                   +gf+f+e+a++ ++ +++ ++e +Gf++va+   +r+k+++++rqg+i+l+l++e++s   a+ f
  lcl|FitnessBrowser__pseudo3_N2E3:AO353_26925  12 MGFEFIELASPTPN-TLEPIFEIMGFTKVAT---HRSKDVHLYRQGQINLILNNEPHSV--ASYF 70 
                                                   58***********9.***************9...************************9..**** PP

                                     TIGR01263  66 lakHGdgvkdvafevedveaafeaavergaeavsapeeedekevklaaikgiGdvvltlverege 130
                                                   +a+HG++v+++af+v+d ++a+++a+e ga++++ ++    +e++l+aikgiG++ l+l++r+ge
  lcl|FitnessBrowser__pseudo3_N2E3:AO353_26925  71 AAEHGPSVCGMAFRVKDSQKAYKRALELGAQPIHIETG--PMELNLPAIKGIGGAPLYLIDRFGE 133
                                                   **********************************9986..99*********************** PP

                                     TIGR01263 131 kgsilpgfeevsekaalkekledvgleaiDHvvgnvergelekvaefyekilgfkeiksfdikte 195
                                                    +si+++++ + e   +++++ ++gl+ iDH+++nv+rg++ ++a+fyek+++f+ei++fdik+e
  lcl|FitnessBrowser__pseudo3_N2E3:AO353_26925 134 GSSIYDIDFVFIEG--VDRHPVGAGLKIIDHLTHNVYRGRMAYWANFYEKLFNFREIRYFDIKGE 196
                                                   *********99997..677789******************************************* PP

                                     TIGR01263 196 asaLkSkvlasaegkvklplnepaskkkksQIeeyleeyeGaGvQHlAlntedivktveelrarg 260
                                                   +++L+Sk++++++g++++plne +s+k  +QIee+l++++G+G+QH+A++++d++kt+++l++ g
  lcl|FitnessBrowser__pseudo3_N2E3:AO353_26925 197 YTGLTSKAMTAPDGMIRIPLNE-ESSKGAGQIEEFLMQFNGEGIQHVAFLSDDLIKTWDHLKKIG 260
                                                   **********************.899*************************************** PP

                                     TIGR01263 261 veflk.ipetYYdnlkervkklvkedleelkelkiLvDrd.eeG...lLLQiFtkpvvdrgtlFf 320
                                                   ++f++ +p+tYY++l+ r+++ + e+++el++++iL+D++ e G   lLLQiF+++++  g++Ff
  lcl|FitnessBrowser__pseudo3_N2E3:AO353_26925 261 MRFMTaPPDTYYEMLEGRLPN-HGEPVNELQARGILLDGSsEAGdkrLLLQIFSETLM--GPVFF 322
                                                   *****9**************7.*****************99888999***********..***** PP

                                     TIGR01263 321 EiIqRkgakGFGegNfkaLfeaiEreqekrgvl 353
                                                   E+IqRkg++GFGegNfkaLfe+iEr+q++rgvl
  lcl|FitnessBrowser__pseudo3_N2E3:AO353_26925 323 EFIQRKGDDGFGEGNFKALFESIERDQVRRGVL 355
                                                   ******************************985 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (353 nodes)
Target sequences:                          1  (358 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.01
# Mc/sec: 7.21
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources