GapMind for catabolism of small carbon sources

 

Aligments for a candidate for atoD in Pseudomonas fluorescens FW300-N2E3

Align Dehydrocarnitine CoA-transferase and acetoacetate CoA-transferase, subunit B (characterized)
to candidate AO353_27940 AO353_27940 succinyl-CoA--3-ketoacid-CoA transferase

Query= reanno::pseudo6_N2E2:Pf6N2E2_2112
         (221 letters)



>lcl|FitnessBrowser__pseudo3_N2E3:AO353_27940 AO353_27940
           succinyl-CoA--3-ketoacid-CoA transferase
          Length = 219

 Score =  406 bits (1044), Expect = e-118
 Identities = 206/219 (94%), Positives = 215/219 (98%)

Query: 1   MALSREQMAQRVAREMQDGYYVNLGIGIPTLVANYIPEGMEVMLQSENGLLGMGAFPTEA 60
           MAL+REQMAQRVAREM+DGYYVNLGIGIPTLVANYIPEGMEVMLQSENGLLGMGAFPT+ 
Sbjct: 1   MALTREQMAQRVAREMKDGYYVNLGIGIPTLVANYIPEGMEVMLQSENGLLGMGAFPTDD 60

Query: 61  EVDADMINAGKQTVTARIGASIFSSAESFAMIRGGHIDLTVLGAFEVDVEGNIASWMIPG 120
           +VDADMINAGKQTVTARIGASIFSSAESFAMIRGGHIDLTVLGAFEVDV GNIASWMIPG
Sbjct: 61  KVDADMINAGKQTVTARIGASIFSSAESFAMIRGGHIDLTVLGAFEVDVHGNIASWMIPG 120

Query: 121 KLVKGMGGAMDLVAGAENIIVTMTHASKDGESKLLPRCSLPLTGAGCIKRVLTDLAYLEI 180
           KLVKGMGGAMDLVAGA+NIIVTMTHASKDGESKLLP CSLPLTGA CIKRVLTDLAYLEI
Sbjct: 121 KLVKGMGGAMDLVAGADNIIVTMTHASKDGESKLLPYCSLPLTGANCIKRVLTDLAYLEI 180

Query: 181 QDGAFILKERAPGVSVEEIVAKTAGKLIVPDHVPEMQFA 219
           ++GAFILKERAPGVSVEEIV+KTAGKLIVP+HVPEMQFA
Sbjct: 181 ENGAFILKERAPGVSVEEIVSKTAGKLIVPEHVPEMQFA 219


Lambda     K      H
   0.318    0.135    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 293
Number of extensions: 5
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 221
Length of database: 219
Length adjustment: 22
Effective length of query: 199
Effective length of database: 197
Effective search space:    39203
Effective search space used:    39203
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 45 (21.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory