GapMind for catabolism of small carbon sources

 

Alignments for a candidate for opuBA in Pseudomonas fluorescens FW300-N2E3

Align BusAA, component of Uptake system for glycine-betaine (high affinity) and proline (low affinity) (OpuAA-OpuABC) or BusAA-ABC of Lactococcus lactis). BusAA, the ATPase subunit, has a C-terminal tandem cystathionine β-synthase (CBS) domain which is the cytoplasmic K+ sensor for osmotic stress (osmotic strength)while the BusABC subunit has the membrane and receptor domains fused to each other (Biemans-Oldehinkel et al., 2006; Mahmood et al., 2006; Gul et al. 2012). An N-terminal amphipathic α-helix of OpuA is necessary for high activity but is not critical for biogenesis or the ionic regulation of transport (characterized)
to candidate AO353_12265 AO353_12265 hypothetical protein

Query= TCDB::Q9RQ06
         (407 letters)



>FitnessBrowser__pseudo3_N2E3:AO353_12265
          Length = 274

 Score =  295 bits (756), Expect = 8e-85
 Identities = 145/263 (55%), Positives = 202/263 (76%)

Query: 4   KVKIEHLTKIFGKRIKTALTMVEQGEPKNEILKKTGATVGVYDTNFEINEGEIFVIMGLS 63
           K++++++ KIFG R   AL M+ Q + K+++L +TG  +GV D +  I  GEIFVIMGLS
Sbjct: 6   KIEVKNVFKIFGDRSADALDMIRQNKSKDQVLAETGCVIGVNDLSLSIATGEIFVIMGLS 65

Query: 64  GSGKSTLLRLLNRLIEPTSGKIFIDDQDVATLNKEDLLQVRRKSMSMVFQNFGLFPHRTI 123
           GSGKSTL+R +NRLI+PTSG I +D  D+   + E L + RR  +SMVFQ+FGL PH+ +
Sbjct: 66  GSGKSTLVRHINRLIDPTSGVILVDGVDILQYDMEALREFRRHKISMVFQSFGLLPHKNV 125

Query: 124 LENTEYGLEVQNVPKEERRKRAEKALDNANLLDFKDQYPKQLSGGMQQRVGLARALANDP 183
           L+N  YGL+V+   K+   +RA+  ++   L  ++++YP QLSGGM+QRVGLARALA D 
Sbjct: 126 LDNVAYGLKVRGESKQVCAERAQHWINTVGLQGYENKYPHQLSGGMRQRVGLARALAADT 185

Query: 184 EILLMDEAFSALDPLIRREMQDELLELQAKFQKTIIFVSHDLNEALRIGDRIAIMKDGKI 243
           +I+LMDEAFSALDPLIR EMQD+LLELQ    KTI+F++HDL+EA+RIG+RIAI+KDG++
Sbjct: 186 DIILMDEAFSALDPLIRAEMQDQLLELQQTLHKTIVFITHDLDEAVRIGNRIAILKDGQL 245

Query: 244 MQIGTGEEILTNPANDYVKTFVE 266
           +Q+GT +EIL +PA++YV  FV+
Sbjct: 246 IQVGTPKEILHSPADEYVDRFVQ 268


Lambda     K      H
   0.316    0.135    0.364 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 305
Number of extensions: 7
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 407
Length of database: 274
Length adjustment: 28
Effective length of query: 379
Effective length of database: 246
Effective search space:    93234
Effective search space used:    93234
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory