Align Cation acetate symporter (characterized, see rationale)
to candidate AO353_01865 AO353_01865 cation/acetate symporter ActP
Query= uniprot:A0A166PH03 (552 letters) >FitnessBrowser__pseudo3_N2E3:AO353_01865 Length = 552 Score = 1021 bits (2641), Expect = 0.0 Identities = 524/552 (94%), Positives = 535/552 (96%) Query: 1 MIRRLMALLSIAAFAPGVWAAEALTGAVQKQPLNVSAILMFVAFVGATLCITYWASKRNN 60 MIRRL+A L IAAFAP VWAAEALTG V KQPLNVSAILMFV FVGATLCITYWASKRN Sbjct: 1 MIRRLLAALGIAAFAPAVWAAEALTGEVHKQPLNVSAILMFVVFVGATLCITYWASKRNK 60 Query: 61 SAADYYAAGGKITGFQNGLAIAGDYMSAASFLGISALVFTSGYDGLIYSIGFLVGWPIIL 120 SAADYYAAGG+ITG QNGLAIAGDYMSAASFLGISALVF SGYDGLIYSIGFLVGWPIIL Sbjct: 61 SAADYYAAGGRITGLQNGLAIAGDYMSAASFLGISALVFASGYDGLIYSIGFLVGWPIIL 120 Query: 121 FLIAERLRNLGKYTFADVASYRLGQTQIRSLSACGSLVVVAFYLIAQMVGAGKLIQLLFG 180 FLIAERLRNLGKYTFADVASYRLGQTQIR+LSACGSLVVVAFYLIAQMVGAGKLIQLLFG Sbjct: 121 FLIAERLRNLGKYTFADVASYRLGQTQIRTLSACGSLVVVAFYLIAQMVGAGKLIQLLFG 180 Query: 181 LDYHVAVILVGILMCMYVLFGGMLATTWVQIIKAVLLLSGASFMALMVMKHVNFDFNALF 240 LDYHVAVILVGILMC+YVLFGGMLATTWVQIIKAVLLLSGASFMALMVMKHV+FDFN LF Sbjct: 181 LDYHVAVILVGILMCLYVLFGGMLATTWVQIIKAVLLLSGASFMALMVMKHVHFDFNTLF 240 Query: 241 SEAIKVHPKGEAIMSPGGLVKDPISAFSLGLALMFGTAGLPHILMRFFTVSDAKEARKSV 300 SEAIKVHPKGEAIMSPGGLVKDPISAFSLGLALMFGTAGLPHILMRFFTVSDAKEARKSV Sbjct: 241 SEAIKVHPKGEAIMSPGGLVKDPISAFSLGLALMFGTAGLPHILMRFFTVSDAKEARKSV 300 Query: 301 LYATGFIGYFYILTFIIGFGAILLVSTNPAFKDAAGALMGGNNMAAVHLANAVGGSVFLG 360 LYATGFIGYFYILTFIIGFGAILLVSTNPAFKDA GAL+GGNNMAAVHLANAVGGS+FLG Sbjct: 301 LYATGFIGYFYILTFIIGFGAILLVSTNPAFKDATGALLGGNNMAAVHLANAVGGSIFLG 360 Query: 361 FISAVAFATILAVVAGLTLAGASAVSHDLYASVIKKGKANEKDEIRVSKITTIALAVLAI 420 FISAVAFATILAVVAGLTLAGA+AVSHDLYASVIKKGKANEKDEIRVSKITTIAL VLAI Sbjct: 361 FISAVAFATILAVVAGLTLAGATAVSHDLYASVIKKGKANEKDEIRVSKITTIALGVLAI 420 Query: 421 GLGILFEKQNIAFMVGLAFSIAASCNFPVLLLSMYWKKLTTRGAMVGGWLGLVSAVGLMV 480 LGILFE QNIAFMVGLAFSIAASCNFPVLLLSMYWKKLTTRGAMVGGW+GLVSAVGLMV Sbjct: 421 VLGILFESQNIAFMVGLAFSIAASCNFPVLLLSMYWKKLTTRGAMVGGWMGLVSAVGLMV 480 Query: 481 LGPTIWVQILGHEKAIFPYEYPALFSMAIAFVGIWFFSVTDKSAEGVNERALFFPQFVRS 540 LGPTIWVQI+GHEKAIFPYEYPALFSMAIAFVGIWFFS+TDKSA NERALFFPQFVRS Sbjct: 481 LGPTIWVQIMGHEKAIFPYEYPALFSMAIAFVGIWFFSITDKSAAADNERALFFPQFVRS 540 Query: 541 QTGLGASGAVSH 552 QTGLGASGAVSH Sbjct: 541 QTGLGASGAVSH 552 Lambda K H 0.327 0.140 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1170 Number of extensions: 42 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 552 Length of database: 552 Length adjustment: 36 Effective length of query: 516 Effective length of database: 516 Effective search space: 266256 Effective search space used: 266256 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory