Alignments for a candidate for rhaT' in Pseudomonas fluorescens FW300-N2E3

GapMind for catabolism of small carbon sources

Alignments for a candidate for rhaT' in Pseudomonas fluorescens FW300-N2E3

Align RhaT, component of Rhamnose porter (Richardson et al., 2004) (Transport activity is dependent on rhamnokinase (RhaK; AAQ92412) activity (Richardson and Oresnik, 2007) This could be an example of group translocation!) (characterized)
to candidate AO353_20820 AO353_20820 sugar ABC transporter ATP-binding protein

Query= TCDB::Q7BSH4
         (512 letters)



>FitnessBrowser__pseudo3_N2E3:AO353_20820
          Length = 517

 Score =  292 bits (747), Expect = 2e-83
 Identities = 176/496 (35%), Positives = 277/496 (55%), Gaps = 12/496 (2%)

Query: 19  AILEMRGISQIFPGVKALDNVSIALHPGTVTALIGENGAGKSTLVKILTGIYRPNEGEIL 78
           A+L + GI + +     L  + + L  G V AL GENGAGKSTL KI+ G+  P  G++ 
Sbjct: 8   AVLSVSGIGKTY-AQPVLTGIDLTLMRGEVLALTGENGAGKSTLSKIIGGLVTPTTGQMQ 66

Query: 79  VDGRPTTFASAQAAIDAGVTAIHQETVLFDELTVAENIFLGHAPRTRFRTIDWQTMNSRS 138
             GR     S   A + G+  + QE  L   L+VAEN+FL + P        W +     
Sbjct: 67  FQGRDYRPGSRTQAEELGIRMVMQELNLLPTLSVAENLFLDNLPSNG----GWISRKQLR 122

Query: 139 KALLTALES----NIDPTIRLKDLSIAQRHLVAIARALSIEARIVIMDEPTAALSRKEID 194
           KA + A+       IDP   + +L I  + +V IAR L  +  ++I+DEPTA L+ +E++
Sbjct: 123 KAAIAAMAQVGLDAIDPDTLVGELGIGHQQMVEIARNLIGDCHVLILDEPTAMLTAREVE 182

Query: 195 DLFRIVRGLKEQGKAILFISHKFDELYEIADDFVVFPRRSRRPVRGVSRKTPQDEIVRMM 254
            LF  +  L+ +G AI++ISH+ +EL  +A    V    +   V  ++     +++V +M
Sbjct: 183 MLFEQITRLQARGVAIIYISHRLEELARVAQRIAVLRDGNLVCVEPMANYN-SEQLVNLM 241

Query: 255 VGRDVENVFPKIDVAIGGPVLEIRNYSHRTEFRDISFTLRKGEILGVYGLIGAGRSELSQ 314
           VGR++          IG P L ++  S   + RD+SF +R GEI G+ GLIGAGR+EL +
Sbjct: 242 VGRELGEHIDLGPRHIGAPALTVKGLSRSDKVRDVSFEVRSGEIFGISGLIGAGRTELLR 301

Query: 315 SLFGITKPLSGKMVL--EGQEITIHSPQDAIRAGIVYVPEERGRHGLALPMPIFQNMTLP 372
            +FG     SG + L    Q ++I SP DA+  GI  + E+R   GL L   I  N+ L 
Sbjct: 302 LIFGADAADSGTVALGSPAQVVSIRSPADAVGHGIALITEDRKGEGLLLTQSISANIALG 361

Query: 373 SLARTSRRGFLRAANEFALARKYAERLDLRAAALSVPVGTLSGGNQQKVVIGKWLATAPK 432
           ++   S  G + + +E ALA++  + + +R+++ +  V  LSGGNQQKVVIG+WL     
Sbjct: 362 NMPVISSGGIVNSGDEMALAQRQIDAMRIRSSSPAQLVSELSGGNQQKVVIGRWLERECS 421

Query: 433 VIILDEPTKGIDIGSKAAVHGFISELAAEGLSIIMVSSELPEIIGMSDRVLVMKEGLSAG 492
           V++ DEPT+GID+G+K  ++  + EL  +G ++++VSS+L E++ + DR+ V+  G    
Sbjct: 422 VLLFDEPTRGIDVGAKFDIYALLGELTRQGKALVVVSSDLRELMLICDRIGVLSAGRLID 481

Query: 493 IFERAELSPEALVRAA 508
            FER   + + L+ AA
Sbjct: 482 TFERDSWTQDDLLAAA 497



 Score = 83.6 bits (205), Expect = 2e-20
 Identities = 69/225 (30%), Positives = 108/225 (48%), Gaps = 11/225 (4%)

Query: 289 ISFTLRKGEILGVYGLIGAGRSELSQSLFGITKPLSGKMVLEGQEITIHSPQDAIRAGIV 348
           I  TL +GE+L + G  GAG+S LS+ + G+  P +G+M  +G++    S   A   GI 
Sbjct: 27  IDLTLMRGEVLALTGENGAGKSTLSKIIGGLVTPTTGQMQFQGRDYRPGSRTQAEELGIR 86

Query: 349 YVPEERGRHGLALPMPIFQNM---TLPSLARTSRRGFLRAANEFALARKYAERLDLRAAA 405
            V +E     L   + + +N+    LPS      R  LR A   A+A+   + +D     
Sbjct: 87  MVMQELN---LLPTLSVAENLFLDNLPSNGGWISRKQLRKAAIAAMAQVGLDAIDPDTL- 142

Query: 406 LSVPVGTLSGGNQQKVVIGKWLATAPKVIILDEPTKGIDIGSKAAVHGFISELAAEGLSI 465
               VG L  G+QQ V I + L     V+ILDEPT  +       +   I+ L A G++I
Sbjct: 143 ----VGELGIGHQQMVEIARNLIGDCHVLILDEPTAMLTAREVEMLFEQITRLQARGVAI 198

Query: 466 IMVSSELPEIIGMSDRVLVMKEGLSAGIFERAELSPEALVRAATG 510
           I +S  L E+  ++ R+ V+++G    +   A  + E LV    G
Sbjct: 199 IYISHRLEELARVAQRIAVLRDGNLVCVEPMANYNSEQLVNLMVG 243


Lambda     K      H
   0.320    0.137    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 623
Number of extensions: 33
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 512
Length of database: 517
Length adjustment: 35
Effective length of query: 477
Effective length of database: 482
Effective search space:   229914
Effective search space used:   229914
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources