GapMind for catabolism of small carbon sources

 

Aligments for a candidate for gtsB in Pseudomonas fluorescens FW300-N2E3

Align ABC transporter for D-Galactose and D-Glucose, permease component 1 (characterized)
to candidate AO353_03390 AO353_03390 sugar ABC transporter permease

Query= reanno::pseudo13_GW456_L13:PfGW456L13_1895
         (302 letters)



>lcl|FitnessBrowser__pseudo3_N2E3:AO353_03390 AO353_03390 sugar ABC
           transporter permease
          Length = 302

 Score =  593 bits (1529), Expect = e-174
 Identities = 292/302 (96%), Positives = 300/302 (99%)

Query: 1   MSSVAVFSKASPFDALQRWLPKLVLAPSMLIVLVGFYGYIIWTFILSFTNSSFMPSYKWV 60
           MSSVAVFSKASPFDALQRWLPKLVLAPSMLIVLVGFYGYIIWTF+LSFTNSSFMPSYKWV
Sbjct: 1   MSSVAVFSKASPFDALQRWLPKLVLAPSMLIVLVGFYGYIIWTFVLSFTNSSFMPSYKWV 60

Query: 61  GLQQYMRLMDNDRWWVASKNLALFGGMFISISLVLGVFLAVLLDQRIRKEGFIRTVYLYP 120
           GLQQY+RLM+NDRWWVASKNLA+FGGMFI ISLVLGVFLAVLLDQRIRKEGFIRT+YLYP
Sbjct: 61  GLQQYVRLMENDRWWVASKNLAVFGGMFIGISLVLGVFLAVLLDQRIRKEGFIRTIYLYP 120

Query: 121 MALSMIVTGTAWKWLLNPGLGLDKMLRDWGWEGFRLDWLVDQDRVVYCLVIAAVWQASGF 180
           MALSMIVTGTAWKWLLNPGLGLDKMLRDWGWEGFRLDWLVDQDRVVYCLVIAAVWQASGF
Sbjct: 121 MALSMIVTGTAWKWLLNPGLGLDKMLRDWGWEGFRLDWLVDQDRVVYCLVIAAVWQASGF 180

Query: 181 VMAMFLAGLRGVDQSIIRAAQVDGASLPTIYLKIVLPSLRPVFFSAFMILAHIAIKSFDL 240
           VMAMFLAGLRGVDQSIIRAAQVDGASLPTIYLKIVLPSLRPVFFSAFMILAHIAIKSFDL
Sbjct: 181 VMAMFLAGLRGVDQSIIRAAQVDGASLPTIYLKIVLPSLRPVFFSAFMILAHIAIKSFDL 240

Query: 241 VAAMTAGGPGYSSDLPAMFMYSFTFSRGQMGIGSASAMLMLGAVLTILVPYLYSELRGKR 300
           VAAMTAGGPGYSSDLPAMFMYSFTFSRGQMGIGSASAM+MLGA+L ILVPYLYSELRGKR
Sbjct: 241 VAAMTAGGPGYSSDLPAMFMYSFTFSRGQMGIGSASAMMMLGAILAILVPYLYSELRGKR 300

Query: 301 HD 302
           H+
Sbjct: 301 HE 302


Lambda     K      H
   0.329    0.141    0.448 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 539
Number of extensions: 9
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 302
Length of database: 302
Length adjustment: 27
Effective length of query: 275
Effective length of database: 275
Effective search space:    75625
Effective search space used:    75625
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory