GapMind for catabolism of small carbon sources

 

Aligments for a candidate for aldA in Pseudomonas fluorescens FW300-N2E3

Align NAD(P)+ L-lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized)
to candidate AO353_19510 AO353_19510 aldehyde dehydrogenase

Query= metacyc::MONOMER-16244
         (495 letters)



>lcl|FitnessBrowser__pseudo3_N2E3:AO353_19510 AO353_19510 aldehyde
           dehydrogenase
          Length = 496

 Score =  413 bits (1062), Expect = e-120
 Identities = 215/496 (43%), Positives = 320/496 (64%), Gaps = 11/496 (2%)

Query: 7   VPIKLPNGTTY-EQPTGLFINNEFVQSKSKKTFGTVSPSTEEEITQVYEAFSEDIDDAVE 65
           +P  LP  + + ++   + I  E+V++   +T    +P+T E +  V +A  +D+D AV 
Sbjct: 3   LPYLLPAVSAFIQRAPRMLIGAEWVEAADGQTMPLHNPATGEVLCVVPKATPDDVDRAVL 62

Query: 66  AATAAFH-SSWSTSDPQVRMKVLYKLADLIDEHADTLAHIEALDNGKSLMCSKG-DVALT 123
           AA  AF  S+W+ + P+ R  +L+KLADL++  A+ LA +E L+NGKS   ++  DV L+
Sbjct: 63  AARNAFDDSAWTRTRPRERQNLLWKLADLMERDAELLAQLECLNNGKSAAVAQVMDVQLS 122

Query: 124 AAYFRSCAGWTDKIKGSVIETG------DTHFNYTRREPIGVCGQIIPWNFPLLMASWKL 177
             + R  AGW  KI+GS +E        D   ++ RRE +GV G I+ WNFPLL+A WKL
Sbjct: 123 IDFLRYMAGWATKIEGSSVEVSLPLMPDDQFHSFIRREAVGVVGAIVAWNFPLLLACWKL 182

Query: 178 GPVLCTGCTTVLKTAESTPLSALYLASLIKEAGAPPGVVNVVSGFGPTAGAPISSHPKIK 237
           GP L TGCT VLK A+ TPL+AL LA L+ EAG P GV NVV+G G +AG+ ++ +P + 
Sbjct: 183 GPALATGCTVVLKPADETPLTALKLAELVLEAGYPSGVFNVVTGTGISAGSALTHNPLVD 242

Query: 238 KVAFTGSTATGRHIMKAAAESNLKKVTLELGGKSPNIVFDDADVKSTIQHLVTGIFYNTG 297
           K+ FTGSTA G+ I K A +S + +VTLELGGKSP IV  DAD+ S      + IF+N G
Sbjct: 243 KLTFTGSTAVGKQIGKIAMDS-MTRVTLELGGKSPTIVMADADLGSAAAGAASAIFFNQG 301

Query: 298 EVCCAGSRIYVQEGIYDKIVSEFKNAAESLKIGDPFKEDTFMGAQTSQLQLDKILKYIDI 357
           +VCCAGSR+YVQ   +D +V++    A ++K+G+       MG   S  Q +++  YI++
Sbjct: 302 QVCCAGSRLYVQRKHFDNVVADIAGIANAMKLGNGLDPSVDMGPLISARQQERVYNYIEM 361

Query: 358 GKKEGATVITGGERFGNKGYFIKPTIFGDVKEDHQIVRDEIFGPVVTITKFKTVEEVIAL 417
           G++ GAT++ GGE+FG  G+F+KPT+  DV + H +V++EIFGPV+    F    + + +
Sbjct: 362 GRESGATIVCGGEQFG-PGFFVKPTVIVDVDQKHSLVQEEIFGPVLVAIPFDDEADALRM 420

Query: 418 ANDSEYGLAAGVHTTNLSTAISVSNKINSGTIWVNTYNDFHPMVPFGGYSQSGIGREMGE 477
           ANDS YGL A + + +L+    +  +I SG++WVN ++   P +PFGGY  SG+GREMG 
Sbjct: 421 ANDSPYGLGASIWSNDLAAVHRMIPRIKSGSVWVNCHSALDPALPFGGYKMSGVGREMGY 480

Query: 478 EALDNYTQVKAVRIGL 493
            A+++YT++K+V I L
Sbjct: 481 AAIEHYTELKSVLIKL 496


Lambda     K      H
   0.316    0.133    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 635
Number of extensions: 32
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 495
Length of database: 496
Length adjustment: 34
Effective length of query: 461
Effective length of database: 462
Effective search space:   212982
Effective search space used:   212982
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory