GapMind for catabolism of small carbon sources

 

Aligments for a candidate for aldA in Pseudomonas fluorescens FW300-N2E3

Align NAD(P)+ L-lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized)
to candidate AO353_29295 AO353_29295 aldehyde dehydrogenase

Query= metacyc::MONOMER-16244
         (495 letters)



>lcl|FitnessBrowser__pseudo3_N2E3:AO353_29295 AO353_29295 aldehyde
           dehydrogenase
          Length = 496

 Score =  353 bits (907), Expect = e-102
 Identities = 197/475 (41%), Positives = 276/475 (58%), Gaps = 5/475 (1%)

Query: 24  FINNEFVQSKSKKTFGTVSPSTEEEITQVYEAFSEDIDDAVEAATAAFHSS-WSTSDPQV 82
           FI+  +  + S+ TF  VSP     +  V      D + AV+ A  AF S  W+   P  
Sbjct: 22  FIDGHYCSALSRDTFECVSPVDGRFLANVASTDEADANAAVQVARRAFDSGVWAKLAPAE 81

Query: 83  RMKVLYKLADLIDEHADTLAHIEALDNGKSLMCSKG-DVALTAAYFRSCAGWTDKIKGSV 141
           R ++L + ADLI  + + LA +E LD GK +  S   D+  TA   R  A   DKI   V
Sbjct: 82  RKRILIRFADLILANQEELALLETLDMGKPISDSMAIDIPATANAIRWSAEAIDKIYDEV 141

Query: 142 IETGDTHFNYTRREPIGVCGQIIPWNFPLLMASWKLGPVLCTGCTTVLKTAESTPLSALY 201
             T         REP GV   I+PWNFPL+MASWK  P L  G + +LK +E +PL+A+ 
Sbjct: 142 AATPHDQLGLVTREPSGVVAAIVPWNFPLIMASWKFAPALAAGNSFILKPSEKSPLTAIR 201

Query: 202 LASLIKEAGAPPGVVNVVSGFGPTAGAPISSHPKIKKVAFTGSTATGRHIMKAAAESNLK 261
           +A L  +AG P GV NV+ GFG T G  ++ H  +  +AFTGSTA  + ++  A +SN+K
Sbjct: 202 IAQLALDAGIPKGVFNVLPGFGHTVGKALALHMDVDVLAFTGSTAIAKQLLIYAGQSNMK 261

Query: 262 KVTLELGGKSPNIVFDDA-DVKSTIQHLVTGIFYNTGEVCCAGSRIYVQEGIYDKIVSEF 320
           +V LE GGKSPN+VF DA D+++      + I +N GEVC AGSR+ V+  I ++ +   
Sbjct: 262 RVWLEAGGKSPNVVFADAPDLRAAATAAASAIAFNQGEVCTAGSRLLVERSIREQFIPLL 321

Query: 321 KNAAESLKIGDPFKEDTFMGAQTSQLQLDKILKYIDIGKKEGATVITGGERF--GNKGYF 378
             A ++ K G     +T +GA   Q QLD +L+YI IGK++GA +I GG R      G +
Sbjct: 322 VEALQAWKPGHALDPETTVGAVVDQRQLDNVLRYISIGKEQGAQLIAGGNRALEDTGGLY 381

Query: 379 IKPTIFGDVKEDHQIVRDEIFGPVVTITKFKTVEEVIALANDSEYGLAAGVHTTNLSTAI 438
           ++P IF  V     I R+EIFGPV+++  F T EE +A+ANDS +GLAAGV T+NLS A 
Sbjct: 382 VEPAIFDGVTNAMTIAREEIFGPVLSLITFDTAEEALAIANDSIFGLAAGVWTSNLSKAH 441

Query: 439 SVSNKINSGTIWVNTYNDFHPMVPFGGYSQSGIGREMGEEALDNYTQVKAVRIGL 493
           + +  + +G++WVN Y+      PFGG+ QSG GR+    A D YT++KA  I L
Sbjct: 442 TFARGLRAGSVWVNQYDGGDMTAPFGGFKQSGNGRDKSLHAFDKYTELKATWIKL 496


Lambda     K      H
   0.316    0.133    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 538
Number of extensions: 27
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 495
Length of database: 496
Length adjustment: 34
Effective length of query: 461
Effective length of database: 462
Effective search space:   212982
Effective search space used:   212982
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory