GapMind for catabolism of small carbon sources

 

Aligments for a candidate for ltaE in Pseudomonas fluorescens FW300-N2E3

Align L-threonine aldolase (EC 4.1.2.5) (characterized)
to candidate AO353_07670 AO353_07670 threonine aldolase

Query= reanno::pseudo3_N2E3:AO353_07670
         (346 letters)



>lcl|FitnessBrowser__pseudo3_N2E3:AO353_07670 AO353_07670 threonine
           aldolase
          Length = 346

 Score =  707 bits (1826), Expect = 0.0
 Identities = 346/346 (100%), Positives = 346/346 (100%)

Query: 1   MTDKSQQFASDNYSGICPEAWAAMEQANQGHQRAYGDDEWTARASDDFRKLFETDCEVFF 60
           MTDKSQQFASDNYSGICPEAWAAMEQANQGHQRAYGDDEWTARASDDFRKLFETDCEVFF
Sbjct: 1   MTDKSQQFASDNYSGICPEAWAAMEQANQGHQRAYGDDEWTARASDDFRKLFETDCEVFF 60

Query: 61  AFNGTAANSLALSSLCQSYHSVICSETAHVETDECGAPEFFSNGSKLLIARTENGKLTPE 120
           AFNGTAANSLALSSLCQSYHSVICSETAHVETDECGAPEFFSNGSKLLIARTENGKLTPE
Sbjct: 61  AFNGTAANSLALSSLCQSYHSVICSETAHVETDECGAPEFFSNGSKLLIARTENGKLTPE 120

Query: 121 SIREIALKRQDIHYPKPRVVTLTQATEVGSIYTPEEIRAISATCKELGLNLHMDGARFSN 180
           SIREIALKRQDIHYPKPRVVTLTQATEVGSIYTPEEIRAISATCKELGLNLHMDGARFSN
Sbjct: 121 SIREIALKRQDIHYPKPRVVTLTQATEVGSIYTPEEIRAISATCKELGLNLHMDGARFSN 180

Query: 181 ACAFLNCSPADLTWKAGVDVLCFGGTKNGMAVGEAILFFNHKLAEDFDYRCKQAGQLASK 240
           ACAFLNCSPADLTWKAGVDVLCFGGTKNGMAVGEAILFFNHKLAEDFDYRCKQAGQLASK
Sbjct: 181 ACAFLNCSPADLTWKAGVDVLCFGGTKNGMAVGEAILFFNHKLAEDFDYRCKQAGQLASK 240

Query: 241 MRFLSAPWVGLLENDAWLKHARHANYCAQLLAELVSDIPGVELMFPVQANGVFLQLSESA 300
           MRFLSAPWVGLLENDAWLKHARHANYCAQLLAELVSDIPGVELMFPVQANGVFLQLSESA
Sbjct: 241 MRFLSAPWVGLLENDAWLKHARHANYCAQLLAELVSDIPGVELMFPVQANGVFLQLSESA 300

Query: 301 VAALTAKSWRFYTFIGKGGARFMCSWDTEVERVRELAADIREVMAG 346
           VAALTAKSWRFYTFIGKGGARFMCSWDTEVERVRELAADIREVMAG
Sbjct: 301 VAALTAKSWRFYTFIGKGGARFMCSWDTEVERVRELAADIREVMAG 346


Lambda     K      H
   0.320    0.132    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 590
Number of extensions: 3
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 346
Length of database: 346
Length adjustment: 29
Effective length of query: 317
Effective length of database: 317
Effective search space:   100489
Effective search space used:   100489
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory