GapMind for catabolism of small carbon sources

 

Alignments for a candidate for glk in Pseudomonas fluorescens FW300-N2E3

Align Glucokinase (EC 2.7.1.2) (characterized)
to candidate AO353_03415 AO353_03415 glucokinase

Query= reanno::WCS417:GFF4431
         (318 letters)



>FitnessBrowser__pseudo3_N2E3:AO353_03415
          Length = 316

 Score =  566 bits (1459), Expect = e-166
 Identities = 276/316 (87%), Positives = 295/316 (93%)

Query: 1   MKLALVGDIGGTNARFALWRDQELHSIRVHATADHSSPEDAIKVYLKEEGLEIGDIGAVC 60
           +KLALVGDIGGTNARFALW+DQ+L SI+V ATAD++ PEDAI VYL   GL  G IGAVC
Sbjct: 1   VKLALVGDIGGTNARFALWKDQQLESIQVLATADYACPEDAIGVYLSGLGLAPGSIGAVC 60

Query: 61  LSVAGPVSGDEFKFTNNHWRLSKTAFCKTLQVDELLLVNDFSAMALGMTRLKPDEFRVVC 120
           LSVAGPVSGDEF+FTNNHWRLS+ AFC+TLQVD LLLVNDFSAMALGMTRL+P EFRVVC
Sbjct: 61  LSVAGPVSGDEFRFTNNHWRLSRKAFCQTLQVDRLLLVNDFSAMALGMTRLQPGEFRVVC 120

Query: 121 EGTPEPLRPAVVIGPGTGLGVGTLLDLGAGRFAALPGEGGHVDLPLSSPRETQLWQHIYT 180
           EGTPEPLRPAVVIGPGTGLGVGTLLDLG GR+AALPGEGGHVDLPLSSPRETQLWQHIY 
Sbjct: 121 EGTPEPLRPAVVIGPGTGLGVGTLLDLGEGRWAALPGEGGHVDLPLSSPRETQLWQHIYN 180

Query: 181 EIGHVSAETALSGGGLPRLYRAICAVDGHTPVLETPEAITAAGLAGDPVAMEVLDQFSIW 240
           EIGHVSAETALSGGGLPR+YRAICAVDGH PVL+TPEAITAAGLAGDP+A+EVL+QF  W
Sbjct: 181 EIGHVSAETALSGGGLPRVYRAICAVDGHEPVLDTPEAITAAGLAGDPIAVEVLEQFCCW 240

Query: 241 LGRVAGNNVLTTGGRGGVYIVGGVIPRFADFFINSGFAKSFADKGCMSDYFKGIPVWLVT 300
           LGRVAGNNVLT GGRGGVYIVGGVIPRFADFF+ SGFA+ FADKGCMS+YFKGIPVWLVT
Sbjct: 241 LGRVAGNNVLTVGGRGGVYIVGGVIPRFADFFLKSGFARCFADKGCMSEYFKGIPVWLVT 300

Query: 301 APYSGLTGAGVALEQA 316
           APYSGLTGAGVALEQA
Sbjct: 301 APYSGLTGAGVALEQA 316


Lambda     K      H
   0.320    0.139    0.429 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 450
Number of extensions: 7
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 318
Length of database: 316
Length adjustment: 27
Effective length of query: 291
Effective length of database: 289
Effective search space:    84099
Effective search space used:    84099
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 48 (23.1 bits)

Align candidate AO353_03415 AO353_03415 (glucokinase)
to HMM TIGR00749 (glk: glucokinase (EC 2.7.1.2))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR00749.hmm
# target sequence database:        /tmp/gapView.32295.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00749  [M=315]
Accession:   TIGR00749
Description: glk: glucokinase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                     Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                     -----------
    4.4e-82  261.8   0.0    5.1e-82  261.6   0.0    1.0  1  lcl|FitnessBrowser__pseudo3_N2E3:AO353_03415  AO353_03415 glucokinase


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo3_N2E3:AO353_03415  AO353_03415 glucokinase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  261.6   0.0   5.1e-82   5.1e-82       1     315 []       5     310 ..       5     310 .. 0.97

  Alignments for each domain:
  == domain 1  score: 261.6 bits;  conditional E-value: 5.1e-82
                                     TIGR00749   1 lvgdiGGtnarlalvevapgeieqvktyssedfpsleavvrvyleeakvelkdpi.kgcfaiatP 64 
                                                   lvgdiGGtnar+al     +++e+++++ + d+   e+++ vyl+      +  i   c+++a+P
  lcl|FitnessBrowser__pseudo3_N2E3:AO353_03415   5 LVGDIGGTNARFAL--WKDQQLESIQVLATADYACPEDAIGVYLSGLGLAPGS-IgAVCLSVAGP 66 
                                                   89************..8899**************************9999998.5489******* PP

                                     TIGR00749  65 iigdfvrltnldWalsieelkqelalaklelindfaavayailalkeedliqlggakveesaaia 129
                                                   + gd  r+tn++W ls +   q+l + +l l+ndf+a+a++++ l+  ++ ++    +e+  +  
  lcl|FitnessBrowser__pseudo3_N2E3:AO353_03415  67 VSGDEFRFTNNHWRLSRKAFCQTLQVDRLLLVNDFSAMALGMTRLQPGEFRVVCEGTPEPLRPAV 131
                                                   ***************************************************************** PP

                                     TIGR00749 130 ilGaGtGlGvatliqqsdgrykvlageGghvdfaPrseleillleylrkkygrvsaervlsGsGl 194
                                                   ++G+GtGlGv tl++ ++gr+ +l+geGghvd+   s+ e++l++ + +++g+vsae  lsG Gl
  lcl|FitnessBrowser__pseudo3_N2E3:AO353_03415 132 VIGPGTGLGVGTLLDLGEGRWAALPGEGGHVDLPLSSPRETQLWQHIYNEIGHVSAETALSGGGL 196
                                                   ***************************************************************** PP

                                     TIGR00749 195 vliyealskrkgerevsklskeelkekdiseaalegsdvlarralelflsilGalagnlalklga 259
                                                     +y+a+   +g++ v  l     +++ i+ a l+g+ ++a + le f+  lG++agn  l++g 
  lcl|FitnessBrowser__pseudo3_N2E3:AO353_03415 197 PRVYRAICAVDGHEPV--L----DTPEAITAAGLAGD-PIAVEVLEQFCCWLGRVAGNNVLTVGG 254
                                                   ***********88866..4....4899********96.78999********************** PP

                                     TIGR00749 260 rGGvyvaGGivPrfiellkkssfraafedkGrlkellasiPvqvvlkkkvGllGag 315
                                                   rGGvy++GG++Prf +++ ks+f   f dkG + e+++ iPv +v     Gl Gag
  lcl|FitnessBrowser__pseudo3_N2E3:AO353_03415 255 RGGVYIVGGVIPRFADFFLKSGFARCFADKGCMSEYFKGIPVWLVTAPYSGLTGAG 310
                                                   ******************************************************97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (315 nodes)
Target sequences:                          1  (316 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 7.73
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory