Align trehalose-specific PTS system, I, HPr, and IIA components (characterized)
to candidate AO353_08465 AO353_08465 phosphoenolpyruvate-protein phosphotransferase
Query= reanno::pseudo3_N2E3:AO353_15995 (844 letters) >FitnessBrowser__pseudo3_N2E3:AO353_08465 Length = 759 Score = 295 bits (755), Expect = 7e-84 Identities = 192/571 (33%), Positives = 302/571 (52%), Gaps = 29/571 (5%) Query: 279 GVCASAGSAFGYVVQV---AERTLEMPEFAADQQLERESLERALMHATQALQRLRDNAAG 335 GV S G+A G V + A+ + + AD + E + A+ ++ L A Sbjct: 180 GVPGSPGAAVGTAVVMLPPADLDVVPDKTIADIEAELGLFKTAIEGVRADMRALSAKLAT 239 Query: 336 EAQAD---IFKAHQELLEDPSLLEQAQALIAEGKSAAFAWNSATEATATLFKSLGSTLLA 392 + + + +F + +L+D SL + +I G+ A A F+ + L Sbjct: 240 QLRPEERALFDVYLMMLDDASLGSEVTTVIKTGQWAQGALRQVVTDHVNRFELMDDAYLR 299 Query: 393 ERALDLMDVGQRVLKLILGVPDGVWELPDQAILIAEQLTPSQTAALDTGKVLGFATVGGG 452 ERA D+ D+G+R+L + PD ILI+E+LTP+ + GK++G +V G Sbjct: 300 ERASDVKDLGRRLLAYLQEERQQTLVYPDNTILISEELTPAMLGEVPEGKLVGLVSVLGS 359 Query: 453 ATSHVAILARALGLPAVCGLPLQVLSLASGTRVLLDADKGELHLDPAVSVIEQLHAKRQQ 512 SHVAILARA+G+P V GL S G ++++D GE++ +P+ + +Q ++ Sbjct: 360 GNSHVAILARAMGIPTVMGLVDLPYSKVDGIQMIVDGYHGEVYTNPSDVLRKQFADVVEE 419 Query: 513 QRQRHQHELENAARAAVTRDGHHFEVTANVASLAETEQAMSLGAEGIGLLRSEFLYQQRS 572 ++Q VT DGH + N LA+ +A GAEG+GL R+E + Sbjct: 420 EKQLALGLDALRDLPCVTIDGHRMPLWVNTGLLADVARAQKRGAEGVGLYRTEVPFMINQ 479 Query: 573 VAPSHDEQAGTYSAIARALGPQRNLVVRTLDVGGDKPLAYVPMDSEANPFLGMRGIRLCL 632 PS EQ Y A PQ + +R+LD+GGDK L+Y P+ E NPFLG RGIR+ L Sbjct: 480 RFPSEKEQLAIYREQLAAFHPQP-VTMRSLDIGGDKSLSYFPI-KEDNPFLGWRGIRVTL 537 Query: 633 ERPQLLREQFRAIL-SSAGLARLHIMLPMVSQLSELRLARLMLEEEALALGLRE------ 685 + P++ Q RA+L +S GL L I+LPM+S EL EEAL L R Sbjct: 538 DHPEIFLVQTRAMLKASEGLNNLRILLPMISGTHEL--------EEALHLIHRAWGEVRD 589 Query: 686 ------LPKLGIMIEVPAAALMADLFAPEVDFFSIGTNDLTQYTLAMDRDHPRLASQADS 739 +P +G+MIE+PAA A +VDF S+G+NDLTQY LA+DR++PR+A D Sbjct: 590 EGTDVPMPPVGVMIEIPAAVYQTKELARQVDFLSVGSNDLTQYLLAVDRNNPRVADLYDY 649 Query: 740 FHPSVLRLIASTVKAAHAHGKWVGVCGALASETLAVPLLLGLGVDELSVSVPLIPAIKAA 799 HP+VL+ + + V+ AHA GK V +CG +A + A LL+ +G D LS++ +P +K Sbjct: 650 LHPAVLQALQTVVRDAHAEGKPVSICGEMAGDPAAAVLLMAMGFDSLSMNATNLPKVKWM 709 Query: 800 IREVELSDCQAIAHQVLGLESAEQVREALSV 830 +R++ LS + + +++ +++ + + +L + Sbjct: 710 LRQINLSWARDLLAELMTIDNPQVIHSSLQL 740 Lambda K H 0.318 0.132 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1266 Number of extensions: 61 Number of successful extensions: 7 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 1 Length of query: 844 Length of database: 759 Length adjustment: 41 Effective length of query: 803 Effective length of database: 718 Effective search space: 576554 Effective search space used: 576554 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 55 (25.8 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory