GapMind for catabolism of small carbon sources

 

Aligments for a candidate for aroP in Pseudomonas fluorescens FW300-N2E3

Align Aromatic amino acid transport protein AroP (characterized, see rationale)
to candidate AO353_05965 AO353_05965 aromatic amino acid transporter

Query= uniprot:Q4KIP0
         (466 letters)



>lcl|FitnessBrowser__pseudo3_N2E3:AO353_05965 AO353_05965 aromatic
           amino acid transporter
          Length = 466

 Score =  799 bits (2064), Expect = 0.0
 Identities = 393/457 (85%), Positives = 427/457 (93%)

Query: 2   DQTLKQGELKRGLKNRHIQLIALGGAIGTGLFLGSAGVLKSAGPSMILGYAIAGFIAFLI 61
           D+ L+QG LKRGLKNRHIQLIALGGAIGTGLFLGSAGVLKSAGPSMILGYAIAGFIAFLI
Sbjct: 3   DEQLQQGVLKRGLKNRHIQLIALGGAIGTGLFLGSAGVLKSAGPSMILGYAIAGFIAFLI 62

Query: 62  MRQLGEMIVEEPVAGSFSHFAHKYWGGYFGFLAGWNYWVLYVLVGMAELTAVGKYVQFWW 121
           MRQLGEMIVEEPVAGSFSHFAH YWG + GFL+GWNYWVLYVLVGMAELTAVGKYVQFWW
Sbjct: 63  MRQLGEMIVEEPVAGSFSHFAHNYWGSFAGFLSGWNYWVLYVLVGMAELTAVGKYVQFWW 122

Query: 122 PEIPTWVSAAVFFVLVNLINMMNVKFFGEAEFWFAIIKVVAIVGMIVLGCYMLFSGSGGS 181
           PE+PTWVSAAVFFVLVNLIN MNVK FGE EFWFAIIKVVAI+GMI LGCYML SG+GG 
Sbjct: 123 PEVPTWVSAAVFFVLVNLINTMNVKVFGEMEFWFAIIKVVAIIGMIALGCYMLVSGTGGP 182

Query: 182 QASVSNLWSHGGFFPNGGTGLLMAMAFIMFSFGGLELVGITAAEAAEPRKVIPKAINQVV 241
           QASVSNLWSHGGFFPNG  GLLMAMAFIMFSFGGLELVGITAAEA+EPRKVIPKAINQVV
Sbjct: 183 QASVSNLWSHGGFFPNGTNGLLMAMAFIMFSFGGLELVGITAAEASEPRKVIPKAINQVV 242

Query: 242 YRVLIFYVGALAVLLSLYPWDELLVSLNAGGDAYSSSPFVKIFSLIGSDAAAQILNFVVL 301
           YRVLIFYVGAL VLLSLYPWD+LL +L A GDAYS SPFV+IF+LIGS+ AAQILNFVVL
Sbjct: 243 YRVLIFYVGALTVLLSLYPWDQLLQTLGASGDAYSGSPFVQIFALIGSNTAAQILNFVVL 302

Query: 302 TAALSVYNSGVYCNSRMLYGLAEQGDAPKALMKLNKQGVPILALGISALITLLCVLVNYL 361
           TAALSVYNSGVYCNSRMLYGLAEQGDAPK+LMKLNKQGVP+ ALGISALIT+LCV+VNY+
Sbjct: 303 TAALSVYNSGVYCNSRMLYGLAEQGDAPKSLMKLNKQGVPLRALGISALITMLCVVVNYV 362

Query: 362 APHEALELLFALVVAALMINWALISLTHLRFRKAMAEQGVVPSFKAFWSPLSNYLCLAFM 421
           AP+EALELLFALVVA+LMINWA+ISLTHL+FRKAM ++G+VP FKAFWSP +NYLCLAFM
Sbjct: 363 APNEALELLFALVVASLMINWAMISLTHLKFRKAMGQRGIVPGFKAFWSPYTNYLCLAFM 422

Query: 422 VMIVGVMWMIPGIRASVYAIPVWVLVIWGFYLLSRAK 458
            MI+ VM +IPG+RASVYAIPVWVL+++ FY +  A+
Sbjct: 423 AMIIYVMLLIPGVRASVYAIPVWVLILFVFYRIRVAR 459


Lambda     K      H
   0.327    0.141    0.440 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 871
Number of extensions: 34
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 466
Length of database: 466
Length adjustment: 33
Effective length of query: 433
Effective length of database: 433
Effective search space:   187489
Effective search space used:   187489
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory