Align Energy-coupling factor transporter ATP-binding protein EcfA1; Short=ECF transporter A component EcfA; EC 7.-.-.- (characterized, see rationale)
to candidate AO353_29310 AO353_29310 transporter
Query= uniprot:P40735 (281 letters) >FitnessBrowser__pseudo3_N2E3:AO353_29310 Length = 380 Score = 131 bits (330), Expect = 2e-35 Identities = 84/217 (38%), Positives = 130/217 (59%), Gaps = 6/217 (2%) Query: 17 RKDAERRALDGVSLQVYEGEWLAIVGHNGSGKSTLARALNGLILPESGDIEVAGIQLTEE 76 +K E A+D VSL +++GE A++G +GSGKSTL R L G P G I + G+ +T+ Sbjct: 30 KKFDETIAVDDVSLDIHQGEIFAMLGGSGSGKSTLLRMLAGFERPTEGRIYLDGVDITDM 89 Query: 77 SVWEVRKKIGMVFQNPDNQFVGTTVRDDVAFGLENNGVPREEMIERVDWAVKQVNMQDFL 136 +E + I M+FQ+ F TV ++AFGL+ + +P E+ RV+ +K V+M + Sbjct: 90 PPYE--RPINMMFQSYA-LFPHMTVAQNIAFGLKQDRLPASEIEARVEEMLKLVHMTQYA 146 Query: 137 DQEPHHLSGGQKQRVAIAGVIAARPDIIILDEATSMLDPIGREEV-LETVRHLKEQGMAT 195 ++PH LSGGQ+QRVA+A +A RP +++LDE LD R ++ LE V ++ G+ T Sbjct: 147 KRKPHQLSGGQRQRVALARSLAKRPKLLLLDEPMGALDKKLRSQMQLELVEIIERVGV-T 205 Query: 196 VISITHDLNEA-AKADRIIVMNGGKKYAEGPPEEIFK 231 + +THD EA A RI +M+ G G P +I++ Sbjct: 206 CVMVTHDQEEAMTMAQRIAIMHLGWIAQIGSPVDIYE 242 Lambda K H 0.316 0.135 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 261 Number of extensions: 16 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 281 Length of database: 380 Length adjustment: 28 Effective length of query: 253 Effective length of database: 352 Effective search space: 89056 Effective search space used: 89056 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory