GapMind for catabolism of small carbon sources

 

L-tyrosine catabolism in Pseudomonas fluorescens FW300-N2E3

Best path

aroP, HPD, hmgA, maiA, fahA, atoA, atoD, atoB

Also see fitness data for the top candidates

Rules

Overview: Tyrosine utilization in GapMind is based on MetaCyc pathway tyrosine degradation I, via homogentisate (link). This pathway requires oxygen. Another pathway via 4-hydroxyphenylacetate is known (link), but the 4-hydroxyphenylpyruvate oxidase has not been linked to sequence. The other MetaCyc pathways do not yield fixed carbon or are not reported in prokaryotes.

19 steps (16 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
aroP L-tyrosine transporter (AroP/FywP) AO353_18555 AO353_05965
HPD 4-hydroxyphenylpyruvate dioxygenase AO353_26925 AO353_06185
hmgA homogentisate dioxygenase AO353_15550
maiA maleylacetoacetate isomerase AO353_15560 AO353_10285
fahA fumarylacetoacetate hydrolase AO353_15555 AO353_24305
atoA acetoacetyl-CoA transferase, A subunit AO353_27935
atoD acetoacetyl-CoA transferase, B subunit AO353_27940
atoB acetyl-CoA C-acetyltransferase AO353_25685 AO353_27945
Alternative steps:
aacS acetoacetyl-CoA synthetase AO353_21765 AO353_20355
Ac3H11_1692 L-tyrosine ABC transporter, ATPase component 2 AO353_17105 AO353_13360
Ac3H11_1693 L-tyrosine ABC transporter, ATPase component 1 AO353_17110 AO353_13355
Ac3H11_1694 L-tyrosine ABC transporter, permease component 2 AO353_17115 AO353_13350
Ac3H11_1695 L-tyrosine ABC transporter, permease component 1 AO353_13345 AO353_17120
Ac3H11_2396 L-tyrosine ABC transporter, substrate-binding component component AO353_13340 AO353_17125
CAT L-tyrosine transporter CAT
MCT10 L-tyrosine transporter MCT10
TAT1 L-tyrosine permease TAT1 AO353_18555
tyrP Tyrosine permease
tyt1 L-tyrosine:Na+ symporter Tyt1 AO353_05685

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory