Protein AO356_05185 in Pseudomonas fluorescens FW300-N2C3
Annotation: FitnessBrowser__pseudo5_N2C3_1:AO356_05185
Length: 281 amino acids
Source: pseudo5_N2C3_1 in FitnessBrowser
Candidate for 21 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| D-cellobiose catabolism | gtsC | hi | ABC transporter for D-Glucose-6-Phosphate, permease component 1 (characterized) | 97% | 100% | 544.3 | ABC transporter for D-Cellobiose and D-Salicin, permease component 1 | 51% | 295.8 |
| D-glucose catabolism | gtsC | hi | ABC transporter for D-Glucose-6-Phosphate, permease component 1 (characterized) | 97% | 100% | 544.3 | ABC transporter for D-Cellobiose and D-Salicin, permease component 1 | 51% | 295.8 |
| lactose catabolism | gtsC | hi | ABC transporter for D-Glucose-6-Phosphate, permease component 1 (characterized) | 97% | 100% | 544.3 | ABC transporter for D-Cellobiose and D-Salicin, permease component 1 | 51% | 295.8 |
| D-maltose catabolism | gtsC | hi | ABC transporter for D-Glucose-6-Phosphate, permease component 1 (characterized) | 97% | 100% | 544.3 | ABC transporter for D-Cellobiose and D-Salicin, permease component 1 | 51% | 295.8 |
| sucrose catabolism | gtsC | hi | ABC transporter for D-Glucose-6-Phosphate, permease component 1 (characterized) | 97% | 100% | 544.3 | ABC transporter for D-Cellobiose and D-Salicin, permease component 1 | 51% | 295.8 |
| trehalose catabolism | gtsC | hi | ABC transporter for D-Glucose-6-Phosphate, permease component 1 (characterized) | 97% | 100% | 544.3 | ABC transporter for D-Cellobiose and D-Salicin, permease component 1 | 51% | 295.8 |
| D-xylose catabolism | gtsC | hi | ABC transporter for D-Glucose-6-Phosphate, permease component 1 (characterized) | 97% | 100% | 544.3 | ABC transporter for D-Galactose and D-Glucose, permease component 2 | 87% | 498.4 |
| D-galactose catabolism | PfGW456L13_1896 | hi | ABC transporter for D-Galactose and D-Glucose, permease component 2 (characterized) | 87% | 100% | 498.4 | ABC transporter for D-Cellobiose and D-Salicin, permease component 1 | 51% | 295.8 |
| D-cellobiose catabolism | SMc04257 | med | ABC transporter for D-Cellobiose and D-Salicin, permease component 1 (characterized) | 51% | 94% | 295 | ABC transporter for D-Glucose-6-Phosphate, permease component 1 | 97% | 544.3 |
| D-mannose catabolism | TT_C0326 | med | Sugar transport system permease protein aka TT_C0326, component of The glucose/mannose porter TTC0326-8 plus MalK1 (ABC protein, shared with 3.A.1.1.25) (characterized) | 49% | 100% | 265.4 | ABC transporter for D-Glucose-6-Phosphate, permease component 1 | 97% | 544.3 |
| L-arabinose catabolism | xacI | lo | Xylose/arabinose import permease protein XacI (characterized, see rationale) | 34% | 94% | 171 | ABC transporter for D-Glucose-6-Phosphate, permease component 1 | 97% | 544.3 |
| D-cellobiose catabolism | msdB2 | lo | Binding-protein-dependent transport systems inner membrane component (characterized, see rationale) | 33% | 93% | 128.6 | ABC transporter for D-Glucose-6-Phosphate, permease component 1 | 97% | 544.3 |
| D-cellobiose catabolism | aglG' | lo | Inner membrane ABC transporter permease protein (characterized, see rationale) | 31% | 54% | 115.5 | ABC transporter for D-Glucose-6-Phosphate, permease component 1 | 97% | 544.3 |
| D-glucose catabolism | aglG' | lo | Inner membrane ABC transporter permease protein (characterized, see rationale) | 31% | 54% | 115.5 | ABC transporter for D-Glucose-6-Phosphate, permease component 1 | 97% | 544.3 |
| lactose catabolism | aglG' | lo | Inner membrane ABC transporter permease protein (characterized, see rationale) | 31% | 54% | 115.5 | ABC transporter for D-Glucose-6-Phosphate, permease component 1 | 97% | 544.3 |
| D-maltose catabolism | aglG | lo | Inner membrane ABC transporter permease protein (characterized, see rationale) | 31% | 54% | 115.5 | ABC transporter for D-Glucose-6-Phosphate, permease component 1 | 97% | 544.3 |
| D-maltose catabolism | aglG' | lo | Inner membrane ABC transporter permease protein (characterized, see rationale) | 31% | 54% | 115.5 | ABC transporter for D-Glucose-6-Phosphate, permease component 1 | 97% | 544.3 |
| sucrose catabolism | aglG | lo | Inner membrane ABC transporter permease protein (characterized, see rationale) | 31% | 54% | 115.5 | ABC transporter for D-Glucose-6-Phosphate, permease component 1 | 97% | 544.3 |
| sucrose catabolism | aglG' | lo | Inner membrane ABC transporter permease protein (characterized, see rationale) | 31% | 54% | 115.5 | ABC transporter for D-Glucose-6-Phosphate, permease component 1 | 97% | 544.3 |
| trehalose catabolism | aglG | lo | Inner membrane ABC transporter permease protein (characterized, see rationale) | 31% | 54% | 115.5 | ABC transporter for D-Glucose-6-Phosphate, permease component 1 | 97% | 544.3 |
| trehalose catabolism | aglG' | lo | Inner membrane ABC transporter permease protein (characterized, see rationale) | 31% | 54% | 115.5 | ABC transporter for D-Glucose-6-Phosphate, permease component 1 | 97% | 544.3 |
Sequence Analysis Tools
View AO356_05185 at FitnessBrowser
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
Sequence
MTSLAAKPSISLSRIAIYAVLILAVLLYLVPLVVMLLTSFKTPEDISTGNLLSWPTVVSG
IGWVKAWATVDGYFWNSIKITVPAVLISTAIGALNGYVLSMWRFRGSQLFFGLLLFGCFL
PFQTVLLPASFTLGKMGLASTTTGLVFVHVVYGLAFTTLFFRNYYVSIPDALVKAARLDG
AGFFTIFRRIILPMSTPIIMVCLIWQFTQIWNDFLFGVVFSSGDSQPITVALNNLVNTST
GAKEYNVDMAAAMIAGLPTLLVYVVAGKYFVRGLTAGAVKG
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory