GapMind for catabolism of small carbon sources

 

Protein AO356_05190 in Pseudomonas fluorescens FW300-N2C3

Annotation: FitnessBrowser__pseudo5_N2C3_1:AO356_05190

Length: 302 amino acids

Source: pseudo5_N2C3_1 in FitnessBrowser

Candidate for 12 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
D-cellobiose catabolism gtsB hi ABC transporter for D-Glucose-6-Phosphate, permease component 2 (characterized) 96% 100% 591.3 ABC transporter for D-Cellobiose and D-Salicin, permease component 2 42% 245.4
D-glucose catabolism gtsB hi ABC transporter for D-Glucose-6-Phosphate, permease component 2 (characterized) 96% 100% 591.3 ABC transporter for D-Cellobiose and D-Salicin, permease component 2 42% 245.4
lactose catabolism gtsB hi ABC transporter for D-Glucose-6-Phosphate, permease component 2 (characterized) 96% 100% 591.3 ABC transporter for D-Cellobiose and D-Salicin, permease component 2 42% 245.4
D-maltose catabolism gtsB hi ABC transporter for D-Glucose-6-Phosphate, permease component 2 (characterized) 96% 100% 591.3 ABC transporter for D-Cellobiose and D-Salicin, permease component 2 42% 245.4
sucrose catabolism gtsB hi ABC transporter for D-Glucose-6-Phosphate, permease component 2 (characterized) 96% 100% 591.3 ABC transporter for D-Cellobiose and D-Salicin, permease component 2 42% 245.4
trehalose catabolism gtsB hi ABC transporter for D-Glucose-6-Phosphate, permease component 2 (characterized) 96% 100% 591.3 ABC transporter for D-Cellobiose and D-Salicin, permease component 2 42% 245.4
D-xylose catabolism gtsB hi ABC transporter for D-Glucose-6-Phosphate, permease component 2 (characterized) 96% 100% 591.3 ABC transporter for D-Galactose and D-Glucose, permease component 1 88% 557.0
D-galactose catabolism PfGW456L13_1895 hi ABC transporter for D-Galactose and D-Glucose, permease component 1 (characterized) 88% 100% 557 ABC transporter for D-Cellobiose and D-Salicin, permease component 2 42% 245.4
D-cellobiose catabolism SMc04258 med ABC transporter for D-Cellobiose and D-Salicin, permease component 2 (characterized) 42% 96% 245.4 ABC transporter for D-Glucose-6-Phosphate, permease component 2 96% 591.3
L-arabinose catabolism xacH lo Xylose/arabinose import permease protein XacH (characterized, see rationale) 34% 89% 156.4 ABC transporter for D-Glucose-6-Phosphate, permease component 2 96% 591.3
D-mannose catabolism TT_C0327 lo Glucose transport system permease protein aka TT_C0327, component of The glucose/mannose porter TTC0326-8 plus MalK1 (ABC protein, shared with 3.A.1.1.25) (characterized) 36% 57% 127.1 ABC transporter for D-Glucose-6-Phosphate, permease component 2 96% 591.3
trehalose catabolism thuF lo Trehalose transport system permease protein SugA (characterized) 30% 94% 120.6 ABC transporter for D-Glucose-6-Phosphate, permease component 2 96% 591.3

Sequence Analysis Tools

View AO356_05190 at FitnessBrowser

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MSSVAVFSKASPFDALQRWLPKLVLAPSMFIVLVGFYGYILWTFVLSFTTSTFLPNYKWA
GLAQYARLMDNDRWWVASKNLVLFGGMFIGITLVIGVLLAVFLDQRIRREGFIRTIYLYP
MALSMIVTGTAWKWLLNPGMGLDKLLRDWGWEGFRLDWLIDPDRVVYCLVIAAVWQASGF
IMAMFLAGLRGVDQSIIRAAQIDGASMPRIYWKVVLPSLRPVFFSAVMILAHIAIKSFDL
VAAMTAGGPGYSSDLPAMFMYSFTFSRGQMGMGSASAILMLGAILAIIVPYLYSELRTKR
HD

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory