GapMind for catabolism of small carbon sources

 

Protein AO356_15225 in Pseudomonas fluorescens FW300-N2C3

Annotation: AO356_15225 aldehyde dehydrogenase

Length: 506 amino acids

Source: pseudo5_N2C3_1 in FitnessBrowser

Candidate for 11 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
4-hydroxybenzoate catabolism adh hi Aldehyde dehydrogenase (EC 1.2.1.3) (characterized) 87% 100% 907.5 NAD+-dependent secondary alcohol dehydrogenase III monomer 68% 704.9
2'-deoxyinosine catabolism adh hi Aldehyde dehydrogenase (EC 1.2.1.3) (characterized) 87% 100% 907.5 NAD+-dependent secondary alcohol dehydrogenase III monomer 68% 704.9
2-deoxy-D-ribose catabolism adh hi Aldehyde dehydrogenase (EC 1.2.1.3) (characterized) 87% 100% 907.5 NAD+-dependent secondary alcohol dehydrogenase III monomer 68% 704.9
ethanol catabolism adh hi Aldehyde dehydrogenase (EC 1.2.1.3) (characterized) 87% 100% 907.5 NAD+-dependent secondary alcohol dehydrogenase III monomer 68% 704.9
L-threonine catabolism adh hi Aldehyde dehydrogenase (EC 1.2.1.3) (characterized) 87% 100% 907.5 NAD+-dependent secondary alcohol dehydrogenase III monomer 68% 704.9
thymidine catabolism adh hi Aldehyde dehydrogenase (EC 1.2.1.3) (characterized) 87% 100% 907.5 NAD+-dependent secondary alcohol dehydrogenase III monomer 68% 704.9
L-tryptophan catabolism adh hi Aldehyde dehydrogenase (EC 1.2.1.3) (characterized) 87% 100% 907.5 NAD+-dependent secondary alcohol dehydrogenase III monomer 68% 704.9
L-fucose catabolism aldA med NAD(P)+ L-lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized) 43% 97% 369.8 Aldehyde dehydrogenase (EC 1.2.1.3) 87% 907.5
L-rhamnose catabolism aldA med NAD(P)+ L-lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized) 43% 97% 369.8 Aldehyde dehydrogenase (EC 1.2.1.3) 87% 907.5
L-threonine catabolism aldA med NAD(P)+ L-lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized) 43% 97% 369.8 Aldehyde dehydrogenase (EC 1.2.1.3) 87% 907.5
L-tryptophan catabolism nbaE lo 2-aminomuconate semialdehyde dehydrogenase (EC 1.2.1.32) (characterized) 39% 99% 346.7 Aldehyde dehydrogenase (EC 1.2.1.3) 87% 907.5

Sequence Analysis Tools

View AO356_15225 at FitnessBrowser

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search PFam (including for weak hits, up to E = 1)

Predict protein localization: PSORTb (Gram negative bacteria)

Predict transmembrane helices: TMHMM

Check the SEED with FIGfam search

Fitness BLAST: loading...

Sequence

MRYAHPGTEGAIVSFKSKYGNYIGGEFVAPVKGQYFTNTSPVNGQPIAEFPRSTAEDIEK
ALDAAHAAADAWGATSAQARSLILLKIADRIEANLETLAITESWDNGKAVRETLNADIPL
AADHFRYFAGCLRAQEGSAAEIDGNTVAYHIHEPLGVVGQIIPWNFPLLMAAWKLAPALA
AGNCVVLKPAEQTPLGICVLMELIGDLLPPGVLNVVQGFGKEAGEALATSKRIAKIAFTG
STPVGSHIMKCAAENIIPSTVELGGKSPNIFFEDIMQAEPSFIEKAAEGLVLAFFNQGEV
CTCPSRALVQESIYDEFMQVVMKKVLQIKRGDPLDTDTMVGAQASEQQFDKILSYLEIAK
GEGAELLTGGKVEKLEGNLATGYYIQPTLLKGTNKMRVFQEEIFGPVVSITTFKDEAEAL
AIANDTEFGLGAGLWTRDINRAYRMGRAIKAGRVWTNCYHLYPAHAAFGGYKKSGVGRET
HKMMLDHYQQTKNLLVSYDINPLGFF

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory