Protein AO356_20255 in Pseudomonas fluorescens FW300-N2C3
Annotation: FitnessBrowser__pseudo5_N2C3_1:AO356_20255
Length: 322 amino acids
Source: pseudo5_N2C3_1 in FitnessBrowser
Candidate for 24 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| D-galactose catabolism | BPHYT_RS16925 | hi | Arabinose ABC transporter permease (characterized, see rationale) | 98% | 100% | 614 | L-arabinose ABC transporter, permease protein AraH | 64% | 384.8 |
| L-arabinose catabolism | araH | hi | L-arabinose ABC transporter, permease protein AraH (characterized) | 64% | 93% | 384.8 | Ribose import permease protein RbsC | 37% | 215.3 |
| D-ribose catabolism | rbsC | lo | Ribose import permease protein RbsC (characterized) | 37% | 97% | 215.3 | L-arabinose ABC transporter, permease protein AraH | 64% | 384.8 |
| xylitol catabolism | PS417_12060 | lo | ABC transporter permease; SubName: Full=Monosaccharide ABC transporter membrane protein, CUT2 family; SubName: Full=Sugar ABC transporter permease (characterized, see rationale) | 35% | 100% | 201.8 | L-arabinose ABC transporter, permease protein AraH | 64% | 384.8 |
| D-xylose catabolism | xylF_Tm | lo | ABC-type transporter, integral membrane subunit, component of Xylose porter (Nanavati et al. 2006). Regulated by xylose-responsive regulator XylR (characterized) | 35% | 94% | 197.2 | L-arabinose ABC transporter, permease protein AraH | 64% | 384.8 |
| D-cellobiose catabolism | mglC | lo | Putative beta-xyloside ABC transporter, permease component, component of Glucose porter. Also bind xylose (Boucher and Noll 2011). Induced by glucose (Frock et al. 2012). Directly regulated by glucose-responsive regulator GluR (characterized) | 37% | 94% | 196.1 | L-arabinose ABC transporter, permease protein AraH | 64% | 384.8 |
| D-glucose catabolism | mglC | lo | Putative beta-xyloside ABC transporter, permease component, component of Glucose porter. Also bind xylose (Boucher and Noll 2011). Induced by glucose (Frock et al. 2012). Directly regulated by glucose-responsive regulator GluR (characterized) | 37% | 94% | 196.1 | L-arabinose ABC transporter, permease protein AraH | 64% | 384.8 |
| lactose catabolism | mglC | lo | Putative beta-xyloside ABC transporter, permease component, component of Glucose porter. Also bind xylose (Boucher and Noll 2011). Induced by glucose (Frock et al. 2012). Directly regulated by glucose-responsive regulator GluR (characterized) | 37% | 94% | 196.1 | L-arabinose ABC transporter, permease protein AraH | 64% | 384.8 |
| D-maltose catabolism | mglC | lo | Putative beta-xyloside ABC transporter, permease component, component of Glucose porter. Also bind xylose (Boucher and Noll 2011). Induced by glucose (Frock et al. 2012). Directly regulated by glucose-responsive regulator GluR (characterized) | 37% | 94% | 196.1 | L-arabinose ABC transporter, permease protein AraH | 64% | 384.8 |
| sucrose catabolism | mglC | lo | Putative beta-xyloside ABC transporter, permease component, component of Glucose porter. Also bind xylose (Boucher and Noll 2011). Induced by glucose (Frock et al. 2012). Directly regulated by glucose-responsive regulator GluR (characterized) | 37% | 94% | 196.1 | L-arabinose ABC transporter, permease protein AraH | 64% | 384.8 |
| trehalose catabolism | mglC | lo | Putative beta-xyloside ABC transporter, permease component, component of Glucose porter. Also bind xylose (Boucher and Noll 2011). Induced by glucose (Frock et al. 2012). Directly regulated by glucose-responsive regulator GluR (characterized) | 37% | 94% | 196.1 | L-arabinose ABC transporter, permease protein AraH | 64% | 384.8 |
| D-xylose catabolism | xylH | lo | Putative beta-xyloside ABC transporter, permease component, component of Glucose porter. Also bind xylose (Boucher and Noll 2011). Induced by glucose (Frock et al. 2012). Directly regulated by glucose-responsive regulator GluR (characterized) | 37% | 94% | 196.1 | L-arabinose ABC transporter, permease protein AraH | 64% | 384.8 |
| myo-inositol catabolism | PS417_11895 | lo | Inositol transport system permease protein (characterized) | 33% | 96% | 180.3 | L-arabinose ABC transporter, permease protein AraH | 64% | 384.8 |
| D-fructose catabolism | frcC | lo | Ribose ABC transport system, permease protein RbsC (characterized, see rationale) | 34% | 88% | 179.5 | L-arabinose ABC transporter, permease protein AraH | 64% | 384.8 |
| sucrose catabolism | frcC | lo | Ribose ABC transport system, permease protein RbsC (characterized, see rationale) | 34% | 88% | 179.5 | L-arabinose ABC transporter, permease protein AraH | 64% | 384.8 |
| D-mannose catabolism | HSERO_RS03645 | lo | ABC-type sugar transport system, permease component protein (characterized, see rationale) | 35% | 82% | 178.7 | L-arabinose ABC transporter, permease protein AraH | 64% | 384.8 |
| D-galactose catabolism | mglC | lo | MglC aka B2148, component of Galactose/glucose (methyl galactoside) porter (characterized) | 35% | 93% | 172.2 | L-arabinose ABC transporter, permease protein AraH | 64% | 384.8 |
| myo-inositol catabolism | iatP | lo | Inositol ABC transport system, permease protein IatP, component of The myoinositol (high affinity)/ D-ribose (low affinity) transporter IatP/IatA/IbpA. The structure of IbpA with myoinositol bound has been solved (characterized) | 32% | 98% | 163.7 | L-arabinose ABC transporter, permease protein AraH | 64% | 384.8 |
| L-rhamnose catabolism | rhaP | lo | RhaP, component of Rhamnose porter (Richardson et al., 2004) (Transport activity is dependent on rhamnokinase (RhaK; AAQ92412) activity (Richardson and Oresnik, 2007) This could be an example of group translocation!) (characterized) | 31% | 93% | 157.5 | L-arabinose ABC transporter, permease protein AraH | 64% | 384.8 |
| L-fucose catabolism | BPHYT_RS34240 | lo | Monosaccharide-transporting ATPase; EC 3.6.3.17; Flags: Precursor (characterized, see rationale) | 31% | 92% | 156.4 | L-arabinose ABC transporter, permease protein AraH | 64% | 384.8 |
| L-rhamnose catabolism | BPHYT_RS34240 | lo | Monosaccharide-transporting ATPase; EC 3.6.3.17; Flags: Precursor (characterized, see rationale) | 31% | 92% | 156.4 | L-arabinose ABC transporter, permease protein AraH | 64% | 384.8 |
| L-rhamnose catabolism | rhaQ | lo | RhaQ (characterized, see rationale) | 30% | 92% | 156 | L-arabinose ABC transporter, permease protein AraH | 64% | 384.8 |
| D-mannose catabolism | frcC | lo | Fructose import permease protein FrcC (characterized) | 30% | 83% | 140.6 | L-arabinose ABC transporter, permease protein AraH | 64% | 384.8 |
| D-ribose catabolism | frcC | lo | Fructose import permease protein FrcC (characterized) | 30% | 83% | 140.6 | L-arabinose ABC transporter, permease protein AraH | 64% | 384.8 |
Sequence Analysis Tools
View AO356_20255 at FitnessBrowser
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
Sequence
MTIQNNALPTARKPLDLRRFLDDWVMLLAAIGIFVLCTLMIDNFLSPLNMRGLGLAISTT
GIAACTMLYCLASGHFDLSVGSVIACAGVVAAVVMRDTNSVFLGVSAALAMGLIVGLING
IVIAKLRVNALITTLATMQIVRGLAYIFANGKAVGVSQESFFVFGNGQLFGVPVPILITI
ACFLFFGWLLNYTTYGRNTMAIGGNQEAALLAGVNVDRTKIIIFAVHGLIGALAGVILAS
RMTSGQPMIGQGFELTVISACVLGGVSLSGGIGMIRHVIAGVLILAIIENAMNLKNIDTF
YQYVIRGSILLLAVVIDRLKQR
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory