GapMind for catabolism of small carbon sources

 

Protein AO356_28210 in Pseudomonas fluorescens FW300-N2C3

Annotation: FitnessBrowser__pseudo5_N2C3_1:AO356_28210

Length: 10307 amino acids

Source: pseudo5_N2C3_1 in FitnessBrowser

Candidate for 1 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-lysine catabolism amaB lo L-2-aminoadipate reductase; Alpha-aminoadipate reductase; Alpha-AR; L-aminoadipate-semialdehyde dehydrogenase; EC 1.2.1.31; EC 1.2.1.95 (characterized) 31% 51% 261.9

Sequence Analysis Tools

View AO356_28210 at FitnessBrowser

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MQFSELMAALSQHPIRLQQDGDNLVILGDDERMDHALWESLTVHKQELLDLVERNGGDWL
SPAFRITPDMLPLADLTQEEIDRIVDTVPGGAGNVQDIYPLAPLQEGILYHHLAAEQGDP
YVLQSLFGADRRTRLDDFAQALQGVIDRHDILRTSVVWEGLDEPVQVVWHEATLALEEVV
LDPAAGDAVWQLQQRFDTRHHRLDVGQAPLMRLICAQDRDNDRWVAILLFHHMAIDHTAL
ELVQGEMQAFLLGQAHQLPDPVPYRNYVAQVRLGVGGDSHEGFFREMLADVDEPTLPLGF
AEVPEGQSVIEEAHLPIDASLSRRLRAQARQLGVSAASLHHLAWARVVGGLSGKQDVVFG
TVLMGRMQGGDGADRALGMFINTLPLRVKLAGHGAREGVKNTHASLTALLGHEHASLALA
QRCSGVAAPAPLFGALLNYRHSSTAQALSSQTLEAWEGLHALGGAEGTNYPLTLSVDDLG
EGFSLSVLALAGMGAQRIAGYMRQALENLVWMLEQQPDMPLQRLSILPASEREQLLVTFN
ATEADYPRQQTVHGLFEEQVQRTPDAVAVIRGEQRLSYRELNERANRQAHYLRKQGVEPD
SRVAICVERSIDMVVGLLAILKAGGGYVPLDPAYPLDRIAYMLEDSAPAAVLTQTATLEL
LAAASMPVINLESGDWQDESVQNPEVTELTSSHLAYVIYTSGSTGLPKGVMIEHRNTVNF
LTWAHRSFDAQTLSKTLFSTSLNFDLAVYECFAPLTSGGSIEVVTNVLELQQGEHDITLI
NTVPSALKALLESGGLGEGVDTVNVAGEALKRSLVETLFEQTQVKRLCNLYGPSETTTYS
SWVSMAREDGFAAHIGKPVANTQFYLLDEHKQPVPLGVPGEIYIGGAGVARGYLNRDDLT
AERFLKDPFSTAPNARMYRTGDLGRYLPDGNIEYLGRNDDQVKIRGFRIELGEIEAKLSQ
HDALKEAVVVAREDVPGDKRLVAYFTQHSPVDIETLRTHLQAQLPAYMVPVAYVRLEALP
LTPNGKLDRKALPAPDLDAVITRGYEAPQGETETTLAQIWQDLLKVERVGRHDHFFELGG
HSLLAVTLIERMRQAGLSADVRVLFGQPTLAALAAAVGSGKEISVPANLIALDCERITPE
LLPLANLSQTTIDRLVATVPGGVRNVQDIYPLAPLQEGILYHHLAAEQGDPYVLQSQFAF
DNREHLDAFIGALQVVIDRHDILRTSIVWEGLDEPMQVVRREARLHLETLDLDPVAGDVA
EQLRQRFDSRHYRLDIAQAPLMRLAYGRDEANHRLVATLLFHHLALDHTALEVVQHEMQA
YLLGEAGQLAEAVPYRNYVAQARLGVNREEHEAFFRDMLGDIDEPTLPFNLQDVQAGGHD
IEEAHQALDDALDQRLRAQARQLGVSAASLVHLAWAQVLAKVSGKQEVVFGTVLMGRMQG
GEGADRALGMFINTLPLRVTLGEQGVKAGVKAVHGQLTALLGHEHASLALAQRCSGVAAP
LPLFSALLNYRHAATTTSNEVSSAWSGMQALGGEERTNYPLTLNVDDLGTGFNLTVLVGQ
EIGAQRICAYMHAALQTLVLALEQTPQAALHRLSILPAAEIEQLLVTFNATEADYPQQQT
IHGLFEEQVQRTPEAVAVIRGEQRLSYGELNERANRLAHYLRKQGVKPDSRVAICVERGI
DMVVGLLAILKAGGGYVPLDPAYPLDRIAYMLEDSAPAAVLAQDATVGLLSDASMAVINL
DSGDWQDESVQNPEVTELTSSHLAYVIYTSGSTGLPKGVMIEHRNTVNFLTWAHRSFDSQ
TLAKTLFSTSLNFDLAVYECFAPLTSGGSIEVVTNVLELQQGEHDITLINTVPSALKALL
ESGGLGEGVDTVNVAGEALKRSLVETLFEQTQVKRLCNLYGPSETTTYSSWVSMAREDGF
AAHIGKPVANTQFYLLDEHKQPVPLGVPGEIYIGGAGVARGYLNRDDLTAERFLKDPFST
APNARMYKTGDLGRYLPDGNIEYLGRNDDQVKIRGFRIELGEIEAKLAQAPNIKETVVLA
REDVPGDKRLVAYFTQHSPVDIEALRTHLQAQLPAYMVPVAYVRLEALPLTPNGKLDRKA
LPAPDLDAVITRGYEAPQGEVEISLAQIWQDLLKVERVGRHDHFFELGGHSLLAVSLIGR
MRQLGLSADVRVLFSQPTLAALAAAVGSGGEVVVPDNGILLGCTRITPSMLPLVALDQQD
IDRLVETVPGGARNVQDIYPLAPLQEGILYHHIAAHTGDPYVLQATFTVADRERLEAFAQ
ALQGVIDRHDILRTSVVWEGLDEPVQVVWRKAQLAVEELVLSAEAGDIAGQLRERFDTLH
YRLDMQQAPLMRIAFAHDPANQRWVAMLLFHHMVLDHTALEVVQHEMQAHLLGQADQLGQ
AVPFRNYVAQARLGVSREEHEGFFRDMLSDVDEPTLPFGLLDVRGDGSDIEEAGLSLPAD
LSRRLRAQARQLGVSAASLHHLAWAQVLGQVSGKQDVVFGTVLMGRMQGGDGAEHALGMF
INTLPLRVGVGAQGVRDGVKATHARLTGLLGHEHASLALAQRCSGVVAPMPLFNALLNYR
HSAAGVASNEALVAWDGIQLLSNEERTNYPLTLSVDDLGEAFHLSALAVPQVGAQRICAY
MNIALEHLVEALEQAPQTPLHSLSILPPAERRQLLVDFNATTRSYPQDRTVHGLFEARAK
ELPEATAVVHNGHSLTYAELNRRANCLTRHLIGLGVQPGDRVAILLERSVGLLVSQLAIL
KCAAVYVPLDVSAPLERQDFMVRDSGARTLLTLSAMAVPEGIARVDLDSVVLETEADHDL
RLIQSTESVAYIMYTSGSTGTPKGVLVPHRAINRLVINNGYAEFNAQDRVAFASNPAFDA
STLDVWAPLLNGGCVVVVDQDVLLTQERFAALLQEQSVSVLWMTAGLFHQYAAGLMSVFA
QLRYLIVGGDVLDPAVIGRVLKEGAPQHLLNGYGPTEATTFTTTHEIKSVGEGGIPIGRP
IGNTRVYVLDANRQPVPIGVAGELYIGGDGVAKGYLNRPELSAEKFVADPFNADPGALLY
RTGDLARWRADGAVDYLGRNDDQVKIRGFRIELGEIEARLGECPGVKDAVVLARQDDAGP
KRLVGYVIPEPGVTLSVHALREQLSSVLADYMLPSAFVVLPAFPLTANGKLDRRELPAPD
AEAYASREYEAPLGKVEQTLAQIWADVLKVEQVGRHDHFFELGGHSLLAVTLIERMRQAG
LSADVRVLFSQPTLAALAAAIGSGREVSVPPNLIPLHCEHITPDLLPLINLSQDAIDRLV
ATVPGGARNVQDIYPLAPLQEGILYHHLAAEQGDPYVLQSQFAFDNRERLDAFIGALQMV
IDRHDILRTSVLWEGLDEPVQVVWRQVLLESQCIVPDATAGDVATQLANRFDARHYRLDL
GRAPLLQLAYARDEAQDRWVAILLFHHMALDHTALDVMQHEMQMHLLGQGEQLGAAMPYR
NYVAQARLGTSEQEHETFFREMLGDIDEPTLPFGLQDVQGEGHGIDEDRQPVDLALSRRL
RVQARHLGVSAASLVHLAWAQVLGKVSGKQEVVFGTVLLGRMQGGDGADRALGMFINTLP
LRVDVGTQDVRSGVKATHGRLTALLGHEHASLALAQRCSGVVAPTPLFSALLNYRHSAVQ
VTQDALSAWSGINALGGDERTNYPLTLNVDDLGDGFFLTVMVDTAIGAQRICGYMQVALE
NLVMALEQAPQTPLHALAILPSAERRQLLETWNTTDVTYADDALIHRQFEGWAASQPDTV
AVVHEEQALTYGELNARANQLAHSLVALGVRPDDRVAICVERGLDMIIGLLGILKAGAGY
VPLDPTYPPERIAYMLHDSSPVAVVTQSQWRDSLPVLPVPTLVLDPTDAGEMNQQPRHNP
DVADLNASHLAYVIYTSGSTGLPKGVMVEHRNVARLFSATQPWFEFGPQDVWALFHSFAF
DFSVWEIWGALTHGGRLLVVPQLVSRSPQYCYALLCEAGVTILNQTPSAFRQLIAAQGES
DLSHSLRQVIFGGEALETGILKPWYARQANARTQLVNMYGITETTVHVTYRPLDAADAQL
AGVSPIGKRIPDLQLYVLDTQREPVPVGVTGEMYVGGGGVSRGYLNRDELTAERFLDNPF
SPEPNARMYRTGDLGRWLADGSIEYLGRNDDQVKIRGFRIELGEIEAKLAACNGVREAVV
VARQDTPGDQRLVAYVIAEEGEPPSAAELRDELLGSLAEYMVPSAFVMLTVFPLTTNGKL
DRKALPVPDQSSVVTREYEAPQGEVEIAIARIWQDLLHVERVGRHDHFFEMGGHSLLAVK
LVERMRQIDLAADVRVLFGQPTLAALAAAVGGCQEVQVPANLIPDDCSRITPDLLPLATL
SQDEIDRVVATVPGGVGNVQDIYALTPLQEGILYHHVAAAKGDPYLQYILFAFDSRARLD
LFAKALQSTVSRHDILRTGVVWEGIDEPVQVVWREARLGFDEIMLDPAAGDIARQLRERL
DPRHYRLDIRQAPMMRIGYAQDVANDRWVGMLLFHHMVDDATSLAMLAAEIGAHMSGGEQ
ALAASVPYRNFVAQARLGVSREEHEAFFHEMLGDVAEPTLPFGLQDVQGDGHDIEEVRQR
VDAELGQRLRVQARQLGVSAASLYHLAWALVLGKVSGKEDVVFGTVLLGRMQGGEGADRA
LGMFINTLPLRVSLGAQGVRAAVKSTHARLTALLGHEHASLVLAQRCSGVAAPTPLFSAL
LNYRHLGAQAPSSEVIAAWEGIQTLEGEERTNYPLTLSVDDLGEGFNLTVMAQAPIGAER
VCNYVQCVVENLVQALEQSPLAPLSGLKILPPAERRQLLESWNGTGVAFAQDALIHAQFE
VWAAAQPEAMAVMFEERTLTYSELNARANQVAHRLLAQGIRPDDRVAICVERGLDMIVGL
LGILKAGAGYVPVDPAYPTERIAYMLRDSMPVALLAHAATHRLLAGVSMPVLQLDDGSWQ
DESVANPQVPGLDSSHLAYVIYTSGSTGLPKGVMVEHRNVARLFSATQPWFDFGQQDVWA
LFHSFAFDFSVWEIWGALTHGGRLLVVPQLVSRSPQDCYALLCETGVTVLNQTPSAFRQL
IVAQGESDLRHSLRQVIFGGEALETGMLKPWYARVGNAGTQLVNMYGITETTVHVTYRAL
EAADAQSTGVSPIGKRIPDLQLYVLDAQREPAPVGVVGEMYVGGAGVSRGYLNRDELTAE
RFLDNPFSPEPNARMYRTGDLGRWLADGSIEYLGRNDDQVKIRGFRIELGEIEATLAACE
GVSEAVVIAREDEPGDKRLVAYVIATEGAQPSAVDLRTQLLRSLAEHMVPSAFVILDAFP
LTTNGKLDRKALPAPDQSAVISREYEAPQGEVETTIARIWQDLLKLGRVGRHDNFFELGG
HSLLAVKLIERMRQVDLAADVRVLFGQPTLAALAAAVGGVREVVVPGNAIADGCTRITPD
MLPLADLDQDAIDRIVATVQGGVTNVQDIYALAPLQEGILYHHLAAEQGDPYVLQVLFGF
DNRERLQAFAQALQSVVSRNDILRTSMVWQGLEAPVQVVWRHATLGLEEIEVDPAGGEVV
QQLHGRFDPRHYRLDIAQAPLMRFAFAQDVANQRWVGMLLFHHMALDHTALEVVVHEMQA
SLLGQAHLLSPAVPYRNHVAQARLGVSREQHEAFFREMLGDIDEPTLPFGLQDVQGDGSG
IEEAHQDVDAALAQRLRAQARQLGVSAASLVHLAWGHVVGRVSGREEVVFGTVLMGRMQG
GDGADRALGMFINTLPLRVSVGAQGARAGVKATHGRLSALLGHEHASLSLAQRCSGVPTS
LPLFSTLLNYRHSAAEAVSDQAISAWQGMQTLHMEERTNYPLCLNVDDLGEGFRFTAQAV
VEVGAQRVCGYMHTALESLVAALEQTPERALRELPIVPAAEREQLLVDFNATHADYPQEQ
TIHGLFEAQVLRTPDAVAVRHGRHQLNYRELNGLSNRLAHYLRKQGVEPDSRVAICVERG
IEMVVGLLAILKAGGGYVPLDPAYPLDRIAYMLEDSAPAAILVQGTTRNLLGETAIPVID
LDKGIWQDEAAPNPQVPGLSSSHLAYVIYTSGSTGLPKGVMIEHRNTVNFLTWAHRSFDA
QILSKTLFSTSLNFDLAVYECFAPLTSGGSIEVVTNVLELQQGEHDITLINTVPSALKAL
LESGGLGEGVDTVNVAGEALKRSLVETLFEQTQVKRLCNLYGPSETTTYSSWVSMAREDG
FAAHIGKPVANTQFYLLDEHKQPVPLGVPGEIYIGGAGVARGYLNRDDLTAERFLKNPFS
TAPNARMYRTGDLGRYLPDGNIEYLGRNDDQVKIRGFRIELGEIEAKLAQHDALKEAVVV
AREDVPGDKRLVAYFTQHSPDETVEIEALRTHLQTQLPAYMVPAVYVRLDALPLTPNGKL
DRKALPAPDLDAVISRGYEAPQGEVETTLAQIWQDLLDLQQVGRHDQFFELGGHSLLAIQ
LISQVRLRLGVELGLTELFAQPQLMGLAQAVALAGRSTLPEIVRVSRKDDLPLSFAQQRL
WFLAQMEGASAAYHIPAGLRLRGVLDRLALQRALDRIVARHEALRTTFVQVRGQEARQCI
APVDVGFALLHHDFCGLADAEGQLHALAAQEATDTFSLEQGPLIRGRLVRLGSEDHVLLL
TMHHIVSDGWSIGVLIKELTALYEAFRHDLDDPLPALTVQYSDYAVWQRRWLSGEVLQTQ
SDYWRQALADAPALLMLPTDRARPAQQDYAGATLPVVFDADLTASLKALSQRHGVTLYMT
MMAAWGALLSRLSGQDEVVIGSPVANRTRSEVEGLIGFFVNTLAVRIDLSGAPTVETLLA
RVKAQALGAQAHQDLPFEQVVEVLKPVRSLAHSPLFQAMLSWQTHDNTELVLGDLKLEGL
GAASNVAKFDVSLELSEIQGQMFGALEYATALFDEGTVQRYLGYFQRLLRAMVASDQTRI
ERIALLDNAEREHLLAGLNATQAAYPREQTIHQLFEAQVEARPDAIAVAFGDERLSYAEL
NRQANQMAHHLIGLGIRPDDRVAICVERGADMLIGLLAILKAGAGYVPLDPAFPAERLAY
MLADSAPVALLSQGELLDRLPAFTVPVVLLDRAERVRCGIAAGRDDNPLVASLGVRHLAY
VIYTSGSTGNPKGVMIEHRGLVNYCVDAVRLFGLTPDDTVLQQNTLNFDLSVEEIFPALL
AGATLAPTRQLFGSAELQQNGDIRPTFLHLTAAHWHTLAAEWHNSPAQARDYLRDVRLIN
VTGDALSTQKLQMWDAVRPAHTRLVNTYGPTEATVSCTAAYMEHDAVDGILGNASIGRPM
ANTRIYLLDAHRQPVPFGVAGEIFIGGDGVARGYLNLDAINAERFLADPFSDQPDARLYK
TGDLARYRPDGRLEYLGRNDFQVKVRGFRIELGEIEARLGDCAGVKEAAVIVREDTPGDK
RLVAYVVPQAGVNLNAASLRAQLAVQLAEYMLPGAFVSLAALPLTPNRKLDRQALPAPDA
EAYASRTHEAPQGHTEIVLAQIWQSLLKVESVGRHDHFFELGGHSLLVMRLIAQVREQLG
VELNLRDVFAQPQLSALAQVLSQAAHSTRPGIVPVSREQALPLSFAQQRLWFLAQLDGTS
AAYHIPTGLRLRGALDTSALTRALDRIVARHEALRTTFVQAQGQEVEQRIAPVDVGFALR
LQALAGRADAETELMALAEEEARQAFDLAHGPLVRGRLVRMADDDHVLVVTLHHIVSDGW
SADLLTHELGVLYEAFRQGQDDPLPALPIQYADYALWQRRWLTGEVLDQQERFWRQTLAD
APALLTLPTDRPRPAQQDHAGAAVGIVLDETLTQGLKALSQRHGATLFMTVMAAWATVLS
RLSGQHDVVIGTPVANRMQAEVEGLIGLFVNTLALRVDVADELTVQALLQQVKVRTLDAQ
AHQDLPFEQVVEVVRPLRSLSHSPVFQAMLSWQNNEATGLELGDMSLQGLGVTSRTAKFD
VLLDMALIDGRLFGALEYATALFDSATMERYLGYLESVLRAMVADEQTLVAQIPLLGDNE
RQNLLETLNATAVDYPQGLTLHGLFEARVVASPDAVAVVSGEAQLSYAQLNERANQIAHR
LLELGIRPDDRVAICADRSLDMVAGLLGILKAGAAYVPLDPDYPHERLAYMLENSAPMVV
LTQRALQASLPATQARTLLLDEAQGFSTQPTDNPQVEGLHAHHLAYVIYTSGSTGLPKGV
MNEHGGVVNRLLWMQDEYGLTATDAVLQKTPFSFDVSVWEFFWPLFSGARLVMARPGGHR
DPAYLREVIQAQGITTLHFVPSMLDVFLAHGEASQCAGLVRVMCSGEALPGHLVRRFKAQ
LPHTGLYNLYGPTEAAVDVTAWDCAGTDTPDSTPIGKPIANTRIYLLDGHQQPVPMGVAG
EIYIGGVQVARGYLNRPELTVERFLRDLFSRAANARMYRTGDLGRYLPDGNIEYLGRNDD
QVKIRGFRIELGEIEAKLAQHEAIKEAVVLALEDVPGAKRLVAYFTVHGDEHSVEIENLR
TQLQAQLPEYMVPAAYVRLQALPLTPNGKLDRKALPAPDLASVITREYEAPQGAVETTLA
QLWAELLKVEKVGRHDHFFELGGHSLLAVTLIERMRQAGLSADVRVLFGQPTLAALAAAV
GGTTEIVVPDNGIVPGCERITPQMLPLADLTQQEVDAIVAWVPGGVSNVQDIYALAPLQE
GILYHHLVSSQGDLYVQQAMFNLEDRARLDAFVSALQGVIDRHDILRTAVLWEGLREPVQ
VVLREAPLAVQTCTVEPGEDVATQLRERFDPRHSRLDVRQAPMMRLACAYDDEHQRWVAL
LLFHHIALDHTALEVIQHDMQAYLLGQQAQLGDAVPYRNYVAQARLGVTQSAHEAFFRDM
LQDIQEPTLPFGLQDVQGDSHVIEQARLGVDDSLNRRLRTQSRLLGVSAASLHHLAWARV
VGAVSGRENVVFGTVLMGRMQGGEGADRALGLFINTLPLRVDVGVDGARAGVKATHARLT
ALLGHEHASLALAQRCSGVATSTPLFSALLNYRHTATQVTEEAQSAWNGIEGLGGEEWSN
YPLSLNIDDLGEAFVLTALAESRIGAERICGYMHTALESLVDALERTPEAPVRELAILPA
AERRQLLVDFNATDTVYPREETVHGLFEAQVRARPDAIALQHGAQQLTYRELHERANHLA
HCLLERGVQPGSRVAIMLNRSFELIISELAILKCAAVYVPLDHHAPEERQRFMLQDSAAV
LVLTTRDRALPEDMPRLDLDTLQLIQAARPAVFPGQTSDTPAYIMYTSGSTGHPKGVIVP
HLAIGRLAINNGYADFGVQDRVAFASNPAFDASTMEVWGALLNGGRVVIIDHETLLSPTQ
FAQALTEAEVSVLFVTTAMFNQYLVLIPDALAGLRILLCGGERGDPASFRRLLAHAPSLR
LVHCYGPTETTTFATTHVVSAVPHGATSVPIGRPIANTRVYILDAARQPVPVGVAGEIHI
GGVGVALGYLNRPDLTEERFIADPFSDSGSARLYRSGDLGRWLPDGTIEYLDRNDGQVKI
RGFRIELGEIEARLHECHGVREALVVAREDSPGDKCLIAYYTEHENGGDLDADVLRAQLR
SVLPEYMIPAAYVRLTALPLTLNGKVNTGALPVPEMHAYGSQDYEAPQGDLEVTLAGLWA
DVLKLEQIGRHDNFFELGGHSLLAIRLVGLLAQANLTVSLAELFQHESVASMASMLLART
EDARVKEAIVPVRTTGQQNPLFLVHEFSGLDLYFPMLGKHIDPDIPVYGLPAVEWGEPQL
QTMECMASRLVGIIRSVQPKGPYRLAGWSFGGVLAYETAIQLIGLDEEVEFVGLIDSYLP
RLVDQGRSRWALDEAHKLHLLDRCDVFWRAALPGEEVLAVMLENLADLQGQLDRFDFTGL
VQHCRDEALLPAELAVYSAEQLWQYLDREVAHGHALAHYAVYPISVPVHLLVAEERKDDA
PEHSGYLGWDAVLPTSKLHCIKVPGNHQSMMQAPHVKALGQAITAALHTAASRPAPSPKS
GYQPLLTIQGGRTDRAPIFCVPGAGDSVTGFIGLTDAFGPDWPIHGLQHRGLDGSTEPFS
LVETAAQVYLDAIDKVQPEGPVHLLGHSFGGWIVFEMAARLHARGRKVASLTLIDSESPG
GNGVVGKPYTATGVLNRLIEAMQLASGKSLGIDATVFGTQDDTAQMRLLHAGMVRAGMLP
QRSAVDSMRGPARAFGTALRTVYQPQHRYTGPVRLVLADDPTLDTAGNKREQEQMIEGWR
KHIPDLSIWYGPGNHFTILKAPHVHNLAAWWQDGLPMLDEEAASDYV

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory