GapMind for catabolism of small carbon sources

 

Protein AO356_29455 in Pseudomonas fluorescens FW300-N2C3

Annotation: AO356_29455 amino acid transporter

Length: 484 amino acids

Source: pseudo5_N2C3_1 in FitnessBrowser

Candidate for 12 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
D-alanine catabolism cycA hi D-serine/D-alanine/glycine transporter (characterized) 58% 94% 532.7 histidine permease 39% 339.3
D-serine catabolism cycA hi D-serine/D-alanine/glycine transporter (characterized) 58% 94% 532.7 L-alanine and D-alanine permease 40% 350.5
L-alanine catabolism cycA hi D-serine/D-alanine/glycine transporter (characterized) 58% 94% 532.7 histidine permease 39% 339.3
L-threonine catabolism RR42_RS28305 med D-serine/D-alanine/glycine transporter (characterized, see rationale) 44% 95% 388.3 D-serine/D-alanine/glycine transporter 58% 532.7
L-serine catabolism serP med Serine transporter, SerP2 or YdgB, of 459 aas and 12 TMSs (Trip et al. 2013). Transports L-alanine (Km = 20 μM), D-alanine (Km = 38 μM), L-serine, D-serine (Km = 356 μM) and glycine (Noens and Lolkema 2015). The encoding gene is adjacent to the one encoding SerP1 (TC# 2.A.3.1.21) (characterized) 41% 98% 330.5 D-serine/L-alanine/D-alanine/glycine/D-cycloserine uptake porter of 556 aas, CycA 51% 490.0
L-histidine catabolism permease lo histidine permease (characterized) 39% 95% 339.3 D-serine/D-alanine/glycine transporter 58% 532.7
L-phenylalanine catabolism aroP lo Phenylalanine:H+ symporter, PheP of 458 aas and 12 established TMSs (characterized) 38% 97% 328.2 D-serine/D-alanine/glycine transporter 58% 532.7
L-threonine catabolism serP1 lo Serine uptake transporter, SerP1, of 259 aas and 12 TMSs (Trip et al. 2013). L-serine is the highest affinity substrate (Km = 18 μM), but SerP1 also transports L-threonine and L-cysteine (Km values = 20 - 40 μM) (characterized) 39% 98% 324.3 D-serine/D-alanine/glycine transporter 58% 532.7
L-tryptophan catabolism aroP lo Aromatic amino acid transport protein AroP (characterized, see rationale) 36% 97% 314.7 D-serine/D-alanine/glycine transporter 58% 532.7
phenylacetate catabolism H281DRAFT_04042 lo Aromatic amino acid transporter AroP (characterized, see rationale) 35% 96% 313.5 D-serine/D-alanine/glycine transporter 58% 532.7
L-tyrosine catabolism aroP lo L-tyrosine transporter (characterized) 37% 96% 312.8 D-serine/D-alanine/glycine transporter 58% 532.7
L-asparagine catabolism ansP lo Asparagine permease (AnsP) of 497 aas and 12 TMSs (characterized) 35% 90% 298.5 D-serine/D-alanine/glycine transporter 58% 532.7

Sequence Analysis Tools

View AO356_29455 at FitnessBrowser

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search PFam (including for weak hits, up to E = 1)

Predict protein localization: PSORTb (Gram negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the SEED with FIGfam search

Fitness BLAST: loading...

Sequence

MKSIPSGLTEQPALQRTLSNRHIQLMAMGGAIGTGLFMGSGKIIALSGTSIILIYMIIGL
FVYFVMRAMGEMLLSNLNFKTFADFAGAYLGPRAAFFLGWSYWLSWSVAVIGDAVVVGGF
FQYWFPDVPAWIPAIGMLMTLFALNVLTVRLFGEIEFWFAIIKIIAVVTLIGVSLVLIAS
SFVSPSGVTASLNHLLDKQAAFPNGLFGFFAGFQMAIFSFAGTELIGTAAAETRNPEKTL
PKAINSIPLRIILFYVLALACIIAVTSWQQVSPSKSPFVELFLVAGFPAAAGIVNFVVLT
SAASSANSGVFSSSRMLFGLANQDNAPGIFRRLSGNSVPLLSLAFTTLLMLVGVLLLFIV
PEVMTAFTIVSTVSAILVIFTWSTILASYIAYRKKRPELHAKSAYKMPGGVPMAWFSLAF
LGFVLCLLALRPDTRIALLVMPGWFIWLAIAYQLTDARKPKSAPGNSEGNAPPPRNITQS
ANIP

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory