Align Acetate/haloacid transporter, Dehp2, with a possible atypical topology (characterized)
to candidate AO356_21725 AO356_21725 MFS transporter
Query= TCDB::F8SVK1 (552 letters) >FitnessBrowser__pseudo5_N2C3_1:AO356_21725 Length = 540 Score = 676 bits (1744), Expect = 0.0 Identities = 320/538 (59%), Positives = 416/538 (77%), Gaps = 14/538 (2%) Query: 11 APMTKEEKRVIFASSLGTVFEWYDFYLAGSLAAFISKSFFSGVNPTAAFIFTLLGFAAGF 70 A ++E ++VIFASSLGTVFEWYDF+L G+LAA ISK FF+GVN T AFIF L+ FAAGF Sbjct: 14 AGTSQETQKVIFASSLGTVFEWYDFFLYGALAAVISKQFFAGVNDTTAFIFALMAFAAGF 73 Query: 71 AVRPFGALVFGRLGDMVGRKYTFLITIVIMGLSTCVVGFLPGYAAIGMASPVIFIAMRLL 130 VRPFGALVFGRLGDM+GRKYTFL TI++MGL+T VG LP YA+IG+A+P+I + +R+L Sbjct: 74 IVRPFGALVFGRLGDMIGRKYTFLATIILMGLATFCVGLLPNYASIGIAAPIILVVLRML 133 Query: 131 QGLALGGEYGGAATYVAEHAPANRRGFYTAWIQTTATLGLFLSLLVILGVRTAMGEDAFG 190 QGLALGGEYGGAATYVAEHAP +RGF+T+WIQ+TATLGL LSLLV+LG R G D F Sbjct: 134 QGLALGGEYGGAATYVAEHAPMGKRGFHTSWIQSTATLGLLLSLLVVLGCRYFTG-DQFE 192 Query: 191 AWGWRIPFVASLVLLGISVWIRMQLHESPAFERIKAEGKTSKAPLSEAFGQWKNLKIVIL 250 WGWRIPF+ S++LLGIS WIR+ LHESPAF ++K EGK KAP+ E+FG+W+NLK+V++ Sbjct: 193 VWGWRIPFLLSILLLGISTWIRLSLHESPAFLKMKEEGKCCKAPIRESFGKWENLKVVLI 252 Query: 251 ALIGVTAGQAVVWYTGQFYALFFLTQTLKVDGASANILIAIALLIGTPFFLFFGSLSDRI 310 AL + AGQAV +Y QFY LFFLTQ LK+D A AN L+ ++++IG PFF+FFG LSD++ Sbjct: 253 ALFSINAGQAVTFYAAQFYVLFFLTQFLKMDPALANSLLIVSVVIGAPFFIFFGWLSDKV 312 Query: 311 GRKPIILAGCLIAALTYFPLFKALTHYANPALEAATQKSPIVVIANPDECSFQFNPVGTS 370 GRKP+++ G L+A YFP+FK L HYANPA++ A++++PI V+A+P C+FQF+PVG + Sbjct: 313 GRKPVLMIGLLLATALYFPIFKTLAHYANPAIDQASRQAPITVVADPATCTFQFDPVGKA 372 Query: 371 KFTSSCDIAKSALSKAGLNYDNVAAPAGTLAQIKVGDTTIDTYDGKAADAKDAGKAFDKN 430 +F S CD K+ L K GL Y +VAAPAG+ Q+ VGD ++ YD A Sbjct: 373 RFDSPCDKVKTFLVKQGLPYSSVAAPAGSAVQVSVGDVKLEGYDEAA------------- 419 Query: 431 LGTALKAASYPPKADPSQLNWPMTVVILTILVIYVTMVYGPIAAMLVEMFPTRIRYTSMS 490 L A+ A YP +AD Q+N PM V ++ L+I M YGP+AA++VE+FPTRIRYTSMS Sbjct: 420 LRGAVTLAGYPQQADAQQINRPMIVALIVALIIISAMCYGPLAALMVELFPTRIRYTSMS 479 Query: 491 LPYHIGNGWFGGFLPATAFAIVAAKGNIYSGLWYPIIIALATFVIGLLFVRETKDSNI 548 LPYHIGNGWFGGFLP +FA+V G+I+ GLWYP++I + V+G++ +RET++ ++ Sbjct: 480 LPYHIGNGWFGGFLPTVSFALVVYTGDIFYGLWYPVVITGVSLVVGMICLRETRNVDL 537 Lambda K H 0.325 0.139 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 849 Number of extensions: 41 Number of successful extensions: 8 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 552 Length of database: 540 Length adjustment: 35 Effective length of query: 517 Effective length of database: 505 Effective search space: 261085 Effective search space used: 261085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory