GapMind for catabolism of small carbon sources

 

Finding step artP for L-arginine catabolism in Pseudomonas fluorescens FW300-N2C3

5 candidates for artP: L-arginine ABC transporter, ATPase component ArtP/HisP/AotP/BgtA

Score Gene Description Similar to Id. Cov. Bits Other hit Other id. Other bits
hi AO356_18715 amino acid transporter L-Arginine ABC transporter, ATPase component (characterized) 100% 100% 497.7
hi AO356_09895 amino acid transporter Probable ATP-binding component of ABC transporter, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR (characterized) 90% 100% 463.4 NocP aka ATU6028 aka AGR_PTI_69, component of Nopaline porter 62% 318.9
hi AO356_05515 amino acid transporter ABC transporter for L-Arginine, putative ATPase component (characterized) 72% 96% 362.5
hi AO356_27105 amino acid transporter ABC transporter for L-Arginine, putative ATPase component (characterized) 71% 96% 348.2
med AO356_18215 amino acid ABC transporter ATP-binding protein Arginine transport ATP-binding protein ArtM (characterized) 57% 100% 273.1 ABC transporter for L-Asparagine and possibly other L-amino acids, putative ATPase component 96% 471.5

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

Also see fitness data for the candidates

Definition of step artP

Or cluster all characterized artP proteins

This GapMind analysis is from Aug 02 2021. The underlying query database was built on Aug 02 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory