GapMind for catabolism of small carbon sources

 

Aligments for a candidate for astC in Pseudomonas fluorescens FW300-N2C3

Align Succinylornithine transaminase (EC 2.6.1.81) (characterized)
to candidate AO356_17010 AO356_17010 acetylornithine aminotransferase

Query= reanno::Koxy:BWI76_RS11670
         (406 letters)



>lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_17010 AO356_17010
           acetylornithine aminotransferase
          Length = 391

 Score =  275 bits (704), Expect = 1e-78
 Identities = 157/377 (41%), Positives = 218/377 (57%), Gaps = 9/377 (2%)

Query: 14  MMPVYAPAAFIPVRGEGSRLWDQQGKEYIDFAGGIAVNALGHAHPRLVKALTEQAGKFWH 73
           +M  Y P A     G G+RLWDQ G+EY+D   G+AV  +GH+HPRLV A++EQAG   H
Sbjct: 6   LMTTYQPLALNFTHGLGTRLWDQDGREYLDAVAGVAVTNVGHSHPRLVAAISEQAGLLLH 65

Query: 74  TGNGYTNEPVLRLAKQLIDATFADRVFFCNSGAEANEAALKLARKYAHDRFGSEKSGIVA 133
           T N Y+ +   RLA++L   +  +R FF NSGAEANE ALKLAR Y   + G E+  +V 
Sbjct: 66  TSNLYSIDWQQRLAQKLTQLSGMERAFFNNSGAEANETALKLARLYGWHK-GIEQPLVVV 124

Query: 134 FKNAFHGRTLFTVSAGGQPAYSQDFAPLPPQIQHAIYNDLDSAKALID---DNTCAVIVE 190
             NAFHGRTL T+SA   P+    +  LP       + DL + + +     +   AV+VE
Sbjct: 125 MDNAFHGRTLGTMSASDGPSVRLGYNRLPGDFIKVPFGDLAALEQIQQAHAERIVAVLVE 184

Query: 191 PMQGEGGVVPADADFLRGLRELCDAHNALLIFDEVQTGVGRTGELYAYMHYGVTPDLLST 250
           P+QGE GV  A   +L+ LR+LC     LL+ DE+QTG+GRTG+ +A+ H G+ PD+++ 
Sbjct: 185 PIQGESGVQLAPPGYLKALRQLCSRRAWLLMLDEIQTGIGRTGQWFAFQHEGIVPDVMTL 244

Query: 251 AKALGGGFPIGALLASERCASVMTVGTHGTTYGGNPLACAVAGEVFATINTREVLNGVKQ 310
           AK LG G PIGA LA  + A + T G+HG+T+GGNPLAC V   V   I  + ++   + 
Sbjct: 245 AKGLGNGVPIGACLARGKAAELFTPGSHGSTFGGNPLACRVGCTVLDIIEEQALVGNARH 304

Query: 311 RHQWFCERLNAINARYGLFKEIRGLGLLIGCVLKDEYAGKAKAISNQAA-EEGLMILIAG 369
           + +    RL    A       IRG GL+IG  LK       + ++ +AA + GL+I +  
Sbjct: 305 QGEQLLSRLRTELAENPNVLAIRGQGLMIGIELKQ----PVRDLTLRAARDHGLLINVTR 360

Query: 370 ANVVRFAPALIISEDEV 386
              +R  P L I   EV
Sbjct: 361 GQTIRLLPPLTIDGREV 377


Lambda     K      H
   0.321    0.137    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 417
Number of extensions: 10
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 406
Length of database: 391
Length adjustment: 31
Effective length of query: 375
Effective length of database: 360
Effective search space:   135000
Effective search space used:   135000
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory