Alignments for a candidate for puuA in Pseudomonas fluorescens FW300-N2C3

GapMind for catabolism of small carbon sources

Alignments for a candidate for puuA in Pseudomonas fluorescens FW300-N2C3

Align Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11) (characterized)
to candidate AO356_04435 AO356_04435 glutamine synthetase

Query= reanno::pseudo5_N2C3_1:AO356_13135
         (458 letters)



>FitnessBrowser__pseudo5_N2C3_1:AO356_04435
          Length = 460

 Score =  394 bits (1011), Expect = e-114
 Identities = 202/449 (44%), Positives = 278/449 (61%), Gaps = 3/449 (0%)

Query: 10  LNEANAFLKEHPEVLYVDLLIADMNGVVRGKRIERTSLHKVYEKGINLPASLFALDINGS 69
           + +A AFL ++P++   +L I D NGV RGK + R  L  VYE G  LP+++  L ING 
Sbjct: 11  VQDALAFLAQNPDIELFELFILDNNGVPRGKLLHRDELLAVYESGRPLPSTILGLTINGD 70

Query: 70  TVESTGLGLDIGDADRICYPIPDTLCNEPWQKRPTAQLLMTMHELEGEPF-FADPREVLR 128
            VE++GL  D+GD D   YPI  +L   PW+  PTA + ++MH  EG P   ADPR +L 
Sbjct: 71  DVENSGLVWDVGDIDCRAYPISGSLQRMPWRLIPTAAVQVSMHPQEGGPATIADPRYLLS 130

Query: 129 QVVTKFDELGLTICAAFELEFYLIDQENVN-GRPQPPRSPISGKRPHSTQVYLIDDLDEY 187
           QV+      G     A ELEFYL+D    N GRPQP R    G RPH+TQVY + +L++ 
Sbjct: 131 QVIEALQADGYYPVMAAELEFYLLDAHRDNQGRPQPARDT-DGGRPHATQVYGLRELEQI 189

Query: 188 VDCLQDILEGAKEQGIPADAIVKESAPAQFEVNLHHVADPIKACDYAVLLKRLIKNIAYD 247
              L D+    K QGIPA   + E AP Q E+ L H +D ++A D AV  KRL+K +A+ 
Sbjct: 190 EPFLADLYAACKLQGIPARTAISEYAPGQVEITLEHRSDALQAMDEAVRYKRLVKGVAHK 249

Query: 248 HEMDTTFMAKPYPGQAGNGLHVHISILDKDGKNIFASEDPEQNAALRHAIGGVLETLPAQ 307
           H M   FMAKP+   AG+G+H+H+S+ D+DG N+FASE  +    LRHA+GG+L+TL   
Sbjct: 250 HGMQACFMAKPFDHLAGSGMHMHVSLADRDGHNLFASEAGDGTPLLRHAVGGMLDTLLDS 309

Query: 308 MAFLCPNVNSYRRFGAQFYVPNSPTWGLDNRTVALRVPTGSADAVRLEHRVAGADANPYL 367
           +   CPN NSYRRF +  Y P + TWG+DNRTV+LRVP G A +  +EHR+ GADANPYL
Sbjct: 310 LLMFCPNANSYRRFQSNSYAPLAATWGVDNRTVSLRVPGGPAFSRHIEHRICGADANPYL 369

Query: 368 LMAAVLAGVHHGLVNKIEPGAPVEGNSYEQNEQSLPNNLRDALRELDDSEVMAKYIDPKY 427
             AA+L G+HHG+  + +PG PVEGN Y Q  + LP +    LR L+ S    +     +
Sbjct: 370 AAAAILGGIHHGIREQCDPGKPVEGNGYAQVIEPLPTDWLTTLRALEASAWARQAFGEAF 429

Query: 428 IDIFVACKESELEEFEHSISDLEYNWYLH 456
           +D+++A K +E  +F   + + ++ WYLH
Sbjct: 430 LDVYLAVKRAEYRQFMGEVGEQDWRWYLH 458


Lambda     K      H
   0.318    0.137    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 624
Number of extensions: 25
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 458
Length of database: 460
Length adjustment: 33
Effective length of query: 425
Effective length of database: 427
Effective search space:   181475
Effective search space used:   181475
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources