Align glutaminase (EC 3.5.1.2) (characterized)
to candidate AO356_21690 AO356_21690 glutaminase A
Query= BRENDA::P0A6W0 (308 letters) >FitnessBrowser__pseudo5_N2C3_1:AO356_21690 Length = 302 Score = 364 bits (935), Expect = e-105 Identities = 183/301 (60%), Positives = 232/301 (77%), Gaps = 1/301 (0%) Query: 8 AILENILRQVRPLIGQGKVADYIPALATVDGSRLGIAICTVDGQLFQAGDAQERFSIQSI 67 A+L IL VRPLIGQGKVADYIPAL TV ++LGIA+ DG+L+ AGDA+ FS+QSI Sbjct: 3 ALLNEILDAVRPLIGQGKVADYIPALGTVVPNQLGIAVYGNDGELYCAGDAETAFSVQSI 62 Query: 68 SKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLVQLEMEQGIPRNPFINAGALVVCDML 127 SKV SLV A+ H S E IW+R+G +PSG PFNSLVQLE E+G PRNPFINAGALV+CD+ Sbjct: 63 SKVFSLVQAIGH-SGEAIWERLGHEPSGQPFNSLVQLEFERGRPRNPFINAGALVICDIN 121 Query: 128 QGRLSAPRQRMLEVVRGLSGVSDISYDTVVARSEFEHSARNAAIAWLMKSFGNFHHDVTT 187 Q R +AP M + VR LSG + D VA SE++H ARNAA+A+LM+SFGNFH+DV Sbjct: 122 QSRFAAPALSMRDFVRRLSGNPQVMVDGKVAESEYQHRARNAAMAYLMQSFGNFHNDVEA 181 Query: 188 VLQNYFHYCALKMSCVELARTFVFLANQGKAIHIDEPVVTPMQARQINALMATSGMYQNA 247 VL++YF +CAL+MSC++LAR F FLAN G H E +++ Q +Q+N++MATSG+Y A Sbjct: 182 VLRSYFSHCALRMSCIDLARAFCFLANDGFCKHSGEQILSARQTQQVNSIMATSGLYDEA 241 Query: 248 GEFAWRVGLPAKSGVGGGIVAIVPHEMAIAVWSPELDDAGNSLAGIAVLEQLTKQLGRSV 307 G FA+RVGLP KSGVGGGIVA+VP + VWSPEL+ AGNSLAG+A LE +++++G SV Sbjct: 242 GNFAYRVGLPGKSGVGGGIVAVVPGRFTVCVWSPELNAAGNSLAGMAALELMSQRIGWSV 301 Query: 308 Y 308 + Sbjct: 302 F 302 Lambda K H 0.321 0.135 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 317 Number of extensions: 15 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 308 Length of database: 302 Length adjustment: 27 Effective length of query: 281 Effective length of database: 275 Effective search space: 77275 Effective search space used: 77275 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory