GapMind for catabolism of small carbon sources

 

Aligments for a candidate for tctC in Pseudomonas fluorescens FW300-N2C3

Align TctC aka STM2786, component of The tricarboxylate transporter, TctABC (characterized)
to candidate AO356_04615 AO356_04615 tricarboxylic transporter

Query= TCDB::Q9FA46
         (325 letters)



>lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_04615 AO356_04615
           tricarboxylic transporter
          Length = 330

 Score =  401 bits (1030), Expect = e-116
 Identities = 193/319 (60%), Positives = 246/319 (77%), Gaps = 4/319 (1%)

Query: 9   LTASILLMSTSVLAQEA---PSRTECIAPAKPGGGFDLTCKLIQVSLLETGAIEKPMRVT 65
           L A  L+ +  +LA +    P R ECIAPA PGGGFDLTCKL+Q +L+    + KPMRVT
Sbjct: 10  LAAGCLMFAGQLLAADPSKEPKRPECIAPASPGGGFDLTCKLVQSALVNQKLLTKPMRVT 69

Query: 66  YMPGGVGAVAYNAIVAQRPGEPGTVVAFSGGSLLNLSQGKFGRYGVDDVRWLASVGTDYG 125
           YMPGGVGAVAYNA+VAQRP + GT+VA+S GSLLNL+QGKFGR+    VRWLA+VGT YG
Sbjct: 70  YMPGGVGAVAYNAVVAQRPADAGTLVAWSSGSLLNLAQGKFGRFDESAVRWLAAVGTSYG 129

Query: 126 MIAVRADSPWKTLKDLMTAMEKDPNSVVIGAGASIGSQDWMKSALLAQKANVDPHKMRYV 185
            IAV++DSP+K L DL+ A++KDP SVVIG+G ++GSQDWM++AL+A+ A ++P  +RYV
Sbjct: 130 AIAVKSDSPYKNLDDLVQALKKDPGSVVIGSGGTVGSQDWMQTALIAKAAGINPRDLRYV 189

Query: 186 AFEGGGEPVTALMGNHVQVVSGDLSEMVPYLGGDKIRVLAVFSENRL-PGQLANIPTAKE 244
           A EGGGE  TAL+G H+QV S D+S+ +P++    +R+LAVF+E R+   ++ NIPTA+E
Sbjct: 190 ALEGGGEIATALLGGHIQVGSTDISDSMPHIQSGDMRLLAVFAEKRIDEPEMKNIPTARE 249

Query: 245 QGYDLVWPIIRGFYVGPKVSDADYQWWVDTFKKLQQTDEFKKQRDLRGLFEFDMTGQQLD 304
           QGYD+VWP++RGFY+GPKVSD DY WW D F KL  ++EF K RD R LF F MTG +LD
Sbjct: 250 QGYDIVWPVVRGFYLGPKVSDEDYAWWKDAFDKLLASEEFAKLRDQRELFPFAMTGPELD 309

Query: 305 DYVKKQVTDYREQAKAFGL 323
            YVKKQV DY+  AK FGL
Sbjct: 310 TYVKKQVADYKVLAKEFGL 328


Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 418
Number of extensions: 11
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 325
Length of database: 330
Length adjustment: 28
Effective length of query: 297
Effective length of database: 302
Effective search space:    89694
Effective search space used:    89694
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory