Alignments for a candidate for H281DRAFT_01113 in Pseudomonas fluorescens FW300-N2C3

GapMind for catabolism of small carbon sources

Alignments for a candidate for H281DRAFT_01113 in Pseudomonas fluorescens FW300-N2C3

Align deoxynucleoside transporter, ATPase component (characterized)
to candidate AO356_00965 AO356_00965 sugar ABC transporter ATP-binding protein

Query= reanno::Burk376:H281DRAFT_01113
         (515 letters)



>FitnessBrowser__pseudo5_N2C3_1:AO356_00965
          Length = 517

 Score =  300 bits (769), Expect = 6e-86
 Identities = 176/481 (36%), Positives = 273/481 (56%), Gaps = 10/481 (2%)

Query: 31  LRGVSLSFQRGQIYHLLGENGCGKSTLIKIISGAQPPDEGQLVIEGVPHARLSALEALAA 90
           L  ++L+  RG++  L GENG GKSTL KII G   P  GQ+  +G  +   S  +A   
Sbjct: 24  LTDINLTLMRGEVLALTGENGAGKSTLSKIIGGLVTPTTGQMQFQGQDYRPGSRTQAEEL 83

Query: 91  GIETVYQDLSLLPNMSVAENVALTSELATHEGRLARTFDRRVLAATAARALEAVGLPGNS 150
           G+  V Q+L+LLP +SVAEN+ L + L +H G ++R    + L   A  A+  VGL    
Sbjct: 84  GVRMVMQELNLLPTLSVAENLFLDN-LPSHGGWISR----KQLRKAAIEAMAQVGLDAID 138

Query: 151 EFQSTLIEQLPLATRQLVAIARAIASEAKFVIMDEPTTSLTQKEVDNLIAVLANLRAQGV 210
               TL+ +L +  +Q+V IAR +  +   +I+DEPT  LT +EV+ L   +  L+A+GV
Sbjct: 139 P--DTLVGELGIGHQQMVEIARNLIGDCHVLILDEPTAMLTAREVEMLFEQITRLQARGV 196

Query: 211 TVLFVSHKLDECYAIGGEVIVLRDGQKMAQGPIAEFTKAQISELMTGRHLSNERYRESAH 270
            ++++SH+L+E   +   + VLRDG  +   P+A +   Q+  LM GR L          
Sbjct: 197 AIIYISHRLEELARVAQRIAVLRDGNLVCVEPMANYDSEQLVTLMVGRELGEHIDLGPRQ 256

Query: 271 AQDIVLDVRGFTRAGQFSDVSFKLHGGEILGVTGLLDSGRNELARALAGVAPAQSGDVLL 330
                L V+G TR+ +  DVSF++  GEI G++GL+ +GR EL R + G  PA SG V L
Sbjct: 257 IGAPALTVKGLTRSDKVRDVSFEVRSGEIFGISGLIGAGRTELLRLIFGADPADSGTVAL 316

Query: 331 DG--QQIALRTPSDAKRHRIGYVPEDRLNEGLFLDKPIRDNVITAMISSLRDRFGQIDRT 388
               + +++R+PSDA  H I  + EDR  EGL L + I  N+    +  +    G ++ +
Sbjct: 317 GSPARVVSIRSPSDAVAHGIALITEDRKGEGLLLTQSIAANIALGNMPEISSA-GLVNGS 375

Query: 389 RAQALAEQTVKELQIATPGVDKPVQSLSGGNQQRVLIGRWLAIDPRVLILHGPTVGVDVG 448
              ALA++ V  ++I +    + V  LSGGNQQ+V+IGRWL  D  V++   PT G+DVG
Sbjct: 376 AELALAQRQVDAMRIRSSSPTQLVSELSGGNQQKVVIGRWLERDCAVMLFDEPTRGIDVG 435

Query: 449 SKDIIYRIMQRLSQRGIGIILISDDLPELLQNCDRILMMKKGHVSAEYRADELSEADLYH 508
           +K  IY ++  L+++G  ++++S DL EL+  CDRI ++  G +   +  D  ++ DL  
Sbjct: 436 AKFDIYALLGELTRQGKALVVVSSDLRELMLICDRIGVLSAGRLIDTFERDSWTQDDLLA 495

Query: 509 A 509
           A
Sbjct: 496 A 496



 Score = 71.6 bits (174), Expect = 6e-17
 Identities = 65/221 (29%), Positives = 106/221 (47%), Gaps = 14/221 (6%)

Query: 23  KRFTGVHALRGVSLSFQRGQIYHLLGENGCGKSTLIKIISGAQPPDEGQLVIEGVPHARL 82
           K  T    +R VS   + G+I+ + G  G G++ L+++I GA P D G + + G P AR+
Sbjct: 265 KGLTRSDKVRDVSFEVRSGEIFGISGLIGAGRTELLRLIFGADPADSGTVAL-GSP-ARV 322

Query: 83  ----SALEALAAGIETVYQDL---SLLPNMSVAENVALTSELATHEGRLARTFDRRVLAA 135
               S  +A+A GI  + +D     LL   S+A N+AL +     E   A   +     A
Sbjct: 323 VSIRSPSDAVAHGIALITEDRKGEGLLLTQSIAANIALGN---MPEISSAGLVNGSAELA 379

Query: 136 TAARALEAVGLPGNSEFQSTLIEQLPLATRQLVAIARAIASEAKFVIMDEPTTSLTQKEV 195
            A R ++A+ +  +S  Q  L+ +L    +Q V I R +  +   ++ DEPT  +     
Sbjct: 380 LAQRQVDAMRIRSSSPTQ--LVSELSGGNQQKVVIGRWLERDCAVMLFDEPTRGIDVGAK 437

Query: 196 DNLIAVLANLRAQGVTVLFVSHKLDECYAIGGEVIVLRDGQ 236
            ++ A+L  L  QG  ++ VS  L E   I   + VL  G+
Sbjct: 438 FDIYALLGELTRQGKALVVVSSDLRELMLICDRIGVLSAGR 478


Lambda     K      H
   0.319    0.135    0.376 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 596
Number of extensions: 30
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 515
Length of database: 517
Length adjustment: 35
Effective length of query: 480
Effective length of database: 482
Effective search space:   231360
Effective search space used:   231360
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources